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Analyst
2014 Mar 21;1396:1364-71. doi: 10.1039/c3an02155f.
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Protein binding for detection of small changes on a nanoparticle surface.
Zeng S
,
Huang YM
,
Chang CE
,
Zhong W
.
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Protein adsorption on nanoparticles is closely associated with the physicochemical properties of particles, in particular, their surface properties. We synthesized two batches of polyacrylic acid-coated nanoparticles under almost identical conditions except for the heating duration and found differences in the head-group structure of the polyacrylic acid. The structure change was confirmed by NMR and MS. The two batches of particles had varied binding affinities to a selected group of proteins. Computational work confirmed that the head group of the polymer on the surface of a nanoparticle could directly interact with a protein, and small structural changes in the head group were sufficient to result in a significant difference in the free energy of binding. Our results demonstrate that protein adsorption is so sensitive to the surface properties of particles that it can reveal even small variations in the structure of a nanoparticle surface ligand, and should be useful for quick assessment of nanoparticle properties.
Abeliovich,
An empirical extremum principle for the hill coefficient in ligand-protein interactions showing negative cooperativity.
2005, Pubmed
Abeliovich,
An empirical extremum principle for the hill coefficient in ligand-protein interactions showing negative cooperativity.
2005,
Pubmed
Ai,
Layer-by-layer capsules for magnetic resonance imaging and drug delivery.
2011,
Pubmed
Bayraktar,
Facial control of nanoparticle binding to cytochrome C.
2007,
Pubmed
Calzolai,
Protein--nanoparticle interaction: identification of the ubiquitin--gold nanoparticle interaction site.
2010,
Pubmed
Casula,
The concept of delayed nucleation in nanocrystal growth demonstrated for the case of iron oxide nanodisks.
2006,
Pubmed
Chang,
Tork: Conformational analysis method for molecules and complexes.
2003,
Pubmed
Chen,
Controlled assembly of eccentrically encapsulated gold nanoparticles.
2008,
Pubmed
Chen,
Functionalized single-walled carbon nanotubes as rationally designed vehicles for tumor-targeted drug delivery.
2008,
Pubmed
Czeslik,
Mechanism of protein binding to spherical polyelectrolyte brushes studied in situ using two-photon excitation fluorescence fluctuation spectroscopy.
2004,
Pubmed
Dai,
High-capacity binding of proteins by poly(acrylic acid) brushes and their derivatives.
2006,
Pubmed
De,
Biomimetic interactions of proteins with functionalized nanoparticles: a thermodynamic study.
2007,
Pubmed
Ge,
One-step synthesis of highly water-soluble magnetite colloidal nanocrystals.
2007,
Pubmed
Ghosh,
Domain specific association of small fluorescent probe trans-3-(4-monomethylaminophenyl)-acrylonitrile (MMAPA) with bovine serum albumin (BSA) and its dissociation from protein binding sites by Ag nanoparticles: spectroscopic and molecular docking study.
2012,
Pubmed
Hlady,
Protein adsorption on solid surfaces.
1996,
Pubmed
Hollmann,
Characterization of a planar poly(acrylic acid) brush as a materials coating for controlled protein immobilization.
2006,
Pubmed
Kievit,
Surface engineering of iron oxide nanoparticles for targeted cancer therapy.
2011,
Pubmed
Kuboniwa,
Solution structure of calcium-free calmodulin.
1995,
Pubmed
Lacerda,
Interaction of gold nanoparticles with common human blood proteins.
2010,
Pubmed
Li,
Probing nanoparticle--protein interaction by capillary electrophoresis.
2010,
Pubmed
Li,
Exploration of possible binding sites of nanoparticles on protein by cross-linking chemistry coupled with mass spectrometry.
2011,
Pubmed
Lind,
Comparison of protein adsorption patterns onto differently charged hydrophilic superparamagnetic iron oxide particles obtained in vitro and ex vivo.
2001,
Pubmed
Liu,
Superparamagnetic nanosystems based on iron oxide nanoparticles for biomedical imaging.
2011,
Pubmed
Lundqvist,
Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts.
2008,
Pubmed
Morris,
AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility.
2009,
Pubmed
Moyano,
Nano meets biology: structure and function at the nanoparticle interface.
2011,
Pubmed
Pedretti,
VEGA--an open platform to develop chemo-bio-informatics applications, using plug-in architecture and script programming.
2004,
Pubmed
Polito,
Resolving the structure of ligands bound to the surface of superparamagnetic iron oxide nanoparticles by high-resolution magic-angle spinning NMR spectroscopy.
2008,
Pubmed
Rabe,
Understanding protein adsorption phenomena at solid surfaces.
2011,
Pubmed
Riehemann,
Nanomedicine--challenge and perspectives.
2009,
Pubmed
Sanhai,
Seven challenges for nanomedicine.
2008,
Pubmed
Tan,
Fabrication of polymer nanocavities with tailored openings.
2009,
Pubmed
Tassa,
Dextran-coated iron oxide nanoparticles: a versatile platform for targeted molecular imaging, molecular diagnostics, and therapy.
2011,
Pubmed
Waddell,
Origin of broad polydispersion in functionalized dendrimers and its effects on cancer-cell binding affinity.
2010,
Pubmed
Weiss,
The Hill equation revisited: uses and misuses.
1997,
Pubmed
Wittemann,
Interaction of proteins with linear polyelectrolytes and spherical polyelectrolyte brushes in aqueous solution.
2006,
Pubmed
Yang,
Microvesicles secreted by macrophages shuttle invasion-potentiating microRNAs into breast cancer cells.
2011,
Pubmed
You,
Isomeric control of protein recognition with amino acid- and dipeptide-functionalized gold nanoparticles.
2008,
Pubmed
You,
Regulation of alpha-chymotrypsin activity on the surface of substrate-functionalized gold nanoparticles.
2006,
Pubmed
Zhou,
Structural confirmation and quantification of individual ligands from the surface of multi-functionalized gold nanoparticles.
2010,
Pubmed