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XB-ART-50173
Virchows Arch A Pathol Anat Histopathol 1987 Jan 01;4112:137-47.
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Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5).

Franke WW , Winter S , von Overbeck J , Gudat F , Heitz PU , Stähli C .


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The epitope recognized by the murine monoclonal antibody (mAB lu-5) recently described as a formaldehyde-resistant, "pan-epithelial marker" of great value in tumour diagnosis is located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution from amphibia to man. As this epitope is not reactive after SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the epitope-bearing protein has been identified by a dot-blot antibody binding assay, using purified proteins in which the epitope is reconstituted. We show that the epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The location of this widespread epitope is discussed with respect to homologies of amino acid sequences of cytokeratins and their conformations.

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Species referenced: Xenopus
Genes referenced: krt12.4
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References [+] :
Achtstaetter, Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting. 1986, Pubmed