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J Neurosci
2014 Aug 27;3435:11792-802. doi: 10.1523/JNEUROSCI.2355-14.2014.
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Fast phasic release properties of dopamine studied with a channel biosensor.
Kress GJ
,
Shu HJ
,
Yu A
,
Taylor A
,
Benz A
,
Harmon S
,
Mennerick S
.
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Few other neurotransmitters are of as intense interest to neuropsychiatry and neurology as dopamine, yet existing techniques to monitor dopamine release leave an important spatiotemporal gap in our understanding. Electrochemistry and fluorescence imaging tools have been developed to fill the gap, but these methods have important limitations. We circumvent these limitations by introducing a dopamine-gated chloride channel into rat dorsal striatal medium spiny neurons, targets of strong dopamine innervation, thereby transforming dopamine from a slow transmitter into a fast transmitter and revealing new opportunities for studying moment-to-moment regulation of dopamine release. We demonstrate pharmacological and biophysical properties of the channel that make it suitable for fast, local dopamine measurements, and we demonstrate for the first time spontaneous and evoked responses to vesicular dopamine release in the dorsal striatum. Evoked dopamine currents were separated into a fast, monosynaptic component and a slower-rising and decaying disynaptic component mediated by nicotinic receptor activation. In summary, LGC-53 represents a dopamine biosensor with properties suitable for temporal separation of distinct dopamine signals in targets of dopamine innervation.
Adrover,
Glutamate and dopamine transmission from midbrain dopamine neurons share similar release properties but are differentially affected by cocaine.
2014, Pubmed
Adrover,
Glutamate and dopamine transmission from midbrain dopamine neurons share similar release properties but are differentially affected by cocaine.
2014,
Pubmed
Alcantara,
Localization of dopamine D2 receptors on cholinergic interneurons of the dorsal striatum and nucleus accumbens of the rat.
2003,
Pubmed
Bormann,
Mechanism of anion permeation through channels gated by glycine and gamma-aminobutyric acid in mouse cultured spinal neurones.
1987,
Pubmed
Cachope,
Selective activation of cholinergic interneurons enhances accumbal phasic dopamine release: setting the tone for reward processing.
2012,
Pubmed
Chisari,
Hydrophobic anions potently and uncompetitively antagonize GABA(A) receptor function in the absence of a conventional binding site.
2011,
Pubmed
,
Xenbase
Contant,
Ultrastructural characterization of the acetylcholine innervation in adult rat neostriatum.
1996,
Pubmed
Cragg,
Variable dopamine release probability and short-term plasticity between functional domains of the primate striatum.
2003,
Pubmed
Daniel,
Functional heterogeneity at dopamine release sites.
2009,
Pubmed
Descarries,
Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: a quantitative autoradiographic and immunocytochemical analysis.
1996,
Pubmed
Ding,
Thalamic gating of corticostriatal signaling by cholinergic interneurons.
2010,
Pubmed
Doucet,
Quantification of the dopamine innervation in adult rat neostriatum.
1986,
Pubmed
Dubinsky,
Development of inhibitory synapses among striatal neurons in vitro.
1989,
Pubmed
Ford,
The time course of dopamine transmission in the ventral tegmental area.
2009,
Pubmed
Gantz,
Spontaneous inhibitory synaptic currents mediated by a G protein-coupled receptor.
2013,
Pubmed
Garcia-Reitböck,
SNARE protein redistribution and synaptic failure in a transgenic mouse model of Parkinson's disease.
2010,
Pubmed
Goto,
The Yin and Yang of dopamine release: a new perspective.
2007,
Pubmed
Gubernator,
Fluorescent false neurotransmitters visualize dopamine release from individual presynaptic terminals.
2009,
Pubmed
Harris,
Ultrastructure of synapses in the mammalian brain.
2012,
Pubmed
Jones,
Presynaptic localisation of the nicotinic acetylcholine receptor beta2 subunit immunoreactivity in rat nigrostriatal dopaminergic neurones.
2001,
Pubmed
Kim,
Loading of oxidizable transmitters into secretory vesicles permits carbon-fiber amperometry.
2000,
Pubmed
Lehmann,
The striatal cholinergic interneuron: synaptic target of dopaminergic terminals?
1983,
Pubmed
Li,
Optimal promoter usage for lentiviral vector-mediated transduction of cultured central nervous system cells.
2010,
Pubmed
Mortensen,
Distinct activities of GABA agonists at synaptic- and extrasynaptic-type GABAA receptors.
2010,
Pubmed
Moulder,
Vesicle pool heterogeneity at hippocampal glutamate and GABA synapses.
2007,
Pubmed
Nelson,
Striatal cholinergic interneurons Drive GABA release from dopamine terminals.
2014,
Pubmed
Overstreet,
Slow desensitization regulates the availability of synaptic GABA(A) receptors.
2000,
Pubmed
Patneau,
Structure-activity relationships for amino acid transmitter candidates acting at N-methyl-D-aspartate and quisqualate receptors.
1990,
Pubmed
Pisani,
Activation of D2-like dopamine receptors reduces synaptic inputs to striatal cholinergic interneurons.
2000,
Pubmed
Ringstad,
Ligand-gated chloride channels are receptors for biogenic amines in C. elegans.
2009,
Pubmed
,
Xenbase
Rodriguez,
Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain.
2013,
Pubmed
Sankaranarayanan,
The use of pHluorins for optical measurements of presynaptic activity.
2000,
Pubmed
Scott,
A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration.
2010,
Pubmed
Seeman,
Dopamine receptor pharmacology.
1994,
Pubmed
Staal,
Dopamine neurons release transmitter via a flickering fusion pore.
2004,
Pubmed
Threlfell,
Striatal dopamine release is triggered by synchronized activity in cholinergic interneurons.
2012,
Pubmed
Tong,
Synaptic desensitization of NMDA receptors by calcineurin.
1995,
Pubmed
Trussell,
Glutamate receptor desensitization and its role in synaptic transmission.
1989,
Pubmed
Wang,
Temporal components of cholinergic terminal to dopaminergic terminal transmission in dorsal striatum slices of mice.
2014,
Pubmed
Zhang,
Ca(2+)-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons.
2002,
Pubmed
Zorumski,
Modulation of excitatory synaptic transmission by low concentrations of glutamate in cultured rat hippocampal neurons.
1996,
Pubmed
Zucker,
Short-term synaptic plasticity.
2002,
Pubmed