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Curr Microbiol
2014 Dec 01;696:794-801. doi: 10.1007/s00284-014-0657-x.
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Magainin 2 induces bacterial cell death showing apoptotic properties.
Lee W
,
Lee DG
.
???displayArticle.abstract??? Magainin 2 is pore-forming antimicrobial peptide on lipid matrix of bacterial membrane, secreted from the skin of the African clawed frog Xenopus laevis. The aim of this study was to investigate a new concept for antibacterial mechanisms called bacterial apoptosis-like cell death. We examined the morphological changes induced by magainin 2 in Escherichia coli, regarding apoptosis. Specifically, phosphatidylserine externalization from the inner to outer membrane surface was detected by Annexin V staining, and DNA fragmentation and chromatin condensation was detected by TUNEL and DAPI assay. We also found much mechanistic evidence to support the hypothesis that magainin 2 induces bacterial apoptosis-like death-including disturbance of membrane detected by DiBAC4(3), caspase activation observed by FITC-VAD-FMK staining, and analyzing the role of RecA in bacterial apoptosis-like death through the RecA expression assay by Western blot-in E. Coli when treated with magainin 2. On the basis of these results, magainin 2 exerts antibacterial activity with a new mechanism which is bacterial apoptosis-like death. Searching antimicrobial agents with novel mechanisms of action can be an effective strategy to coping with the emergence of new resistance mechanisms. Magainin 2 deserves further research as a potential antimicrobial therapeutic agent.
Adikesavan,
Separation of recombination and SOS response in Escherichia coli RecA suggests LexA interaction sites.
2011, Pubmed
Adikesavan,
Separation of recombination and SOS response in Escherichia coli RecA suggests LexA interaction sites.
2011,
Pubmed
Becnel Boyd,
Relationships among ciprofloxacin, gatifloxacin, levofloxacin, and norfloxacin MICs for fluoroquinolone-resistant Escherichia coli clinical isolates.
2009,
Pubmed
Chen,
Hyperosmolarity-induced cornification of human corneal epithelial cells is regulated by JNK MAPK.
2008,
Pubmed
Drlica,
DNA gyrase, topoisomerase IV, and the 4-quinolones.
1997,
Pubmed
Dwyer,
Antibiotic-induced bacterial cell death exhibits physiological and biochemical hallmarks of apoptosis.
2012,
Pubmed
Engelberg-Kulka,
Bacterial programmed cell death and multicellular behavior in bacteria.
2006,
Pubmed
Erental,
Two programmed cell death systems in Escherichia coli: an apoptotic-like death is inhibited by the mazEF-mediated death pathway.
2012,
Pubmed
Furuya,
Antimicrobial-resistant bacteria in the community setting.
2006,
Pubmed
Gavrieli,
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
1992,
Pubmed
Hakansson,
Apoptosis-like death in bacteria induced by HAMLET, a human milk lipid-protein complex.
2011,
Pubmed
Huisman,
Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.
1984,
Pubmed
Hwang,
Silver nanoparticles induce apoptotic cell death in Candida albicans through the increase of hydroxyl radicals.
2012,
Pubmed
Imura,
Action mechanism of PEGylated magainin 2 analogue peptide.
2007,
Pubmed
,
Xenbase
Jacobson,
Programmed cell death in animal development.
1997,
Pubmed
Klepser,
Influence of test conditions on antifungal time-kill curve results: proposal for standardized methods.
1998,
Pubmed
Kohanski,
A common mechanism of cellular death induced by bactericidal antibiotics.
2007,
Pubmed
Lemasters,
Mitochondrial calcium and the permeability transition in cell death.
2009,
Pubmed
Liao,
Assessment of the effect of amphotericin B on the vitality of Candida albicans.
1999,
Pubmed
Madeo,
A yeast mutant showing diagnostic markers of early and late apoptosis.
1997,
Pubmed
Madeo,
A caspase-related protease regulates apoptosis in yeast.
2002,
Pubmed
Matsuzaki,
Magainins as paradigm for the mode of action of pore forming polypeptides.
1998,
Pubmed
,
Xenbase
Matsuzaki,
Control of cell selectivity of antimicrobial peptides.
2009,
Pubmed
Miller,
SOS response induction by beta-lactams and bacterial defense against antibiotic lethality.
2004,
Pubmed
Park,
Antifungal effect with apoptotic mechanism(s) of Styraxjaponoside C.
2009,
Pubmed
Phillips,
Apoptosis induced by environmental stresses and amphotericin B in Candida albicans.
2003,
Pubmed
Smrz,
Non-apoptotic phosphatidylserine externalization induced by engagement of glycosylphosphatidylinositol-anchored proteins.
2007,
Pubmed
Talanian,
Substrate specificities of caspase family proteases.
1997,
Pubmed
Thornberry,
Caspases: enemies within.
1998,
Pubmed
Vercammen,
Are metacaspases caspases?
2007,
Pubmed
Wenk,
Magainin 2 amide interaction with lipid membranes: calorimetric detection of peptide binding and pore formation.
1998,
Pubmed
,
Xenbase
Wu,
Plagiochin E, an antifungal active macrocyclic bis(bibenzyl), induced apoptosis in Candida albicans through a metacaspase-dependent apoptotic pathway.
2010,
Pubmed
Yan,
Two hits are better than one: membrane-active and DNA binding-related double-action mechanism of NK-18, a novel antimicrobial peptide derived from mammalian NK-lysin.
2013,
Pubmed
Zasloff,
Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor.
1987,
Pubmed
,
Xenbase
