Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Transgenic Res
2014 Aug 01;234:601-7. doi: 10.1007/s11248-014-9798-8.
Show Gene links
Show Anatomy links
Molecular cloning, characterization and functional assessment of the myosin light polypeptide chain 2 (mylz2) promoter of farmed carp, Labeo rohita.
Mohanta R
,
Jayasankar P
,
Das Mahapatra K
,
Saha JN
,
Barman HK
.
???displayArticle.abstract???
We cloned the 5'-flanking region (1.2 kb) of a muscle-specific gene, encoding myosin light chain 2 polypeptide (mylz2) of a farmed carp, Labeo rohita (rohu). Sequence analysis using TRANSFAC-database search identified the consensus cis acting regulatory elements of TATA-box and E (CANNTG)-box, including the monocyte enhancer factor 2 motif, implying that it is likely to be a functional promoter. The proximal promoter (~620 bp) was highly homologous with that of Danio rerio (zebrafish) as compared to Channa striatus (snakehead murrel) counterparts and showed less identity with Sparus auratus (gilthead sea bream), Xenopus laevis (African clawed frog) and Rattus norvegicus (Norway rat). Direct muscular (skeletal) injection of the construct containing the mylz2 promoter (0.6 kb) fused to a green fluorescent protein (GFP) reporter gene showed efficient expression in L. rohita, validating its functional activity. Further, the functional activity was confirmed by the observation that this promoter drove GFP expression in the skeletal muscle of transgenic rohu. The promoter may have potential applications for value-addition in ornamental fishes and studying gene regulatory functions.
Barman,
Identification and characterization of differentially expressed transcripts in the gills of freshwater prawn (Macrobrachium rosenbergii) under salt stress.
2012, Pubmed
Barman,
Identification and characterization of differentially expressed transcripts in the gills of freshwater prawn (Macrobrachium rosenbergii) under salt stress.
2012,
Pubmed
Devlin,
Interface of biotechnology and ecology for environmental risk assessments of transgenic fish.
2006,
Pubmed
Gong,
Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle.
2003,
Pubmed
Gong,
Transgenic fish in aquaculture and developmental biology.
1995,
Pubmed
Gossett,
A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.
1989,
Pubmed
Hansen,
Strong expression of foreign genes following direct injection into fish muscle.
1991,
Pubmed
Hirayama,
Two types of mRNA encoding myosin regulatory light chain in carp fast skeletal muscle differ in their 3' non-coding regions and expression patterns following temperature acclimation.
1998,
Pubmed
Ju,
Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter.
2003,
Pubmed
Kobiyama,
Molecular cloning and developmental expression patterns of the MyoD and MEF2 families of muscle transcription factors in the carp.
1998,
Pubmed
Krasnov,
Cloning of rainbow trout (Oncorhynchus mykiss) alpha-actin, myosin regulatory light chain genes and the 5'-flanking region of alpha-tropomyosin. Functional assessment of promoters.
2003,
Pubmed
Mohapatra,
Cloning of cDNA and prediction of peptide structure of Plzf expressed in the spermatogonial cells of Labeo rohita.
2010,
Pubmed
Moutou,
Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation.
2001,
Pubmed
Muir,
Possible ecological risks of transgenic organism release when transgenes affect mating success: sexual selection and the Trojan gene hypothesis.
1999,
Pubmed
Nam,
Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis.
2001,
Pubmed
Pan,
Ornamental expression of red fluorescent protein in transgenic founders of white skirt tetra (Gymnocorymbus ternetzi).
2008,
Pubmed
Panda,
Isolation of enriched carp spermatogonial stem cells from Labeo rohita testis for in vitro propagation.
2011,
Pubmed
Quandt,
MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data.
1995,
Pubmed
Rambabu,
Efficient expression of transgenes in adult zebrafish by electroporation.
2005,
Pubmed
Wingender,
TRANSFAC: an integrated system for gene expression regulation.
2000,
Pubmed
Yazawa,
Characterization of promoter activities of four different Japanese flounder promoters in transgenic zebrafish.
2005,
Pubmed
Zeng,
Faithful expression of living color reporter genes in transgenic medaka under two tissue-specific zebrafish promoters.
2005,
Pubmed
