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2014 Jun 01;156:700-16. doi: 10.1111/tra.12164.
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The CryoCapsule: simplifying correlative light to electron microscopy.
Heiligenstein X
,
Heiligenstein J
,
Delevoye C
,
Hurbain I
,
Bardin S
,
Paul-Gilloteaux P
,
Sengmanivong L
,
Régnier G
,
Salamero J
,
Antony C
,
Raposo G
.
???displayArticle.abstract???
Correlating complementary multiple scale images of the same object is a straightforward means to decipher biological processes. Light microscopy and electron microscopy are the most commonly used imaging techniques, yet despite their complementarity, the experimental procedures available to correlate them are technically complex. We designed and manufactured a new device adapted to many biological specimens, the CryoCapsule, that simplifies the multiple sample preparation steps, which at present separate live cell fluorescence imaging from contextual high-resolution electron microscopy, thus opening new strategies for full correlative light to electron microscopy. We tested the biological application of this highly optimized tool on three different specimens: the in vitro Xenopus laevis mitotic spindle, melanoma cells over-expressing YFP-langerin sequestered in organized membranous subcellular organelles and a pigmented melanocytic cell in which the endosomal system was labeled with internalized fluorescent transferrin.
Al-Amoudi,
Cryo-electron microscopy of vitreous sections.
2004, Pubmed
Al-Amoudi,
Cryo-electron microscopy of vitreous sections.
2004,
Pubmed
Betzig,
Imaging intracellular fluorescent proteins at nanometer resolution.
2006,
Pubmed
Brown,
Capturing endocytic segregation events with HPF-CLEM.
2012,
Pubmed
Delevoye,
AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis.
2009,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Dubochet,
Cryo-electron microscopy of vitrified specimens.
1988,
Pubmed
Guizetti,
Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells.
2010,
Pubmed
Hawes,
Rapid freeze-substitution preserves membranes in high-pressure frozen tissue culture cells.
2007,
Pubmed
Jiménez,
Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM.
2010,
Pubmed
Jiménez,
Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers.
2006,
Pubmed
Karreman,
Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen.
2012,
Pubmed
Kremer,
Computer visualization of three-dimensional image data using IMOD.
1996,
Pubmed
Kukulski,
Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision.
2011,
Pubmed
Kukulski,
Plasma membrane reshaping during endocytosis is revealed by time-resolved electron tomography.
2012,
Pubmed
Leforestier,
Comparison of slam-freezing and high-pressure freezing effects on the DNA cholesteric liquid crystalline structure.
1996,
Pubmed
Lenormand,
Birbeck granule-like "organized smooth endoplasmic reticulum" resulting from the expression of a cytoplasmic YFP-tagged langerin.
2013,
Pubmed
Mankidy,
Biological impact of phthalates.
2013,
Pubmed
Matias,
Cryo-transmission electron microscopy of frozen-hydrated sections of Escherichia coli and Pseudomonas aeruginosa.
2003,
Pubmed
McDonald,
Recent advances in high-pressure freezing: equipment- and specimen-loading methods.
2007,
Pubmed
McDonald,
"Tips and tricks" for high-pressure freezing of model systems.
2010,
Pubmed
McDonald,
Cryopreparation methods for electron microscopy of selected model systems.
2007,
Pubmed
Mironov,
Visualizing membrane traffic in vivo by combined video fluorescence and 3D electron microscopy.
2000,
Pubmed
Moor,
The influence of high pressure freezing on mammalian nerve tissue.
1980,
Pubmed
Müller-Reichert,
Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis.
2007,
Pubmed
Müller-Reichert,
Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure.
2003,
Pubmed
Nixon,
A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos.
2009,
Pubmed
Salmon,
Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells.
1976,
Pubmed
Spiegelhalter,
From dynamic live cell imaging to 3D ultrastructure: novel integrated methods for high pressure freezing and correlative light-electron microscopy.
2010,
Pubmed
Studer,
High pressure freezing comes of age.
1989,
Pubmed
Studer,
A new approach for cryofixation by high-pressure freezing.
2001,
Pubmed
Thévenaz,
A pyramid approach to subpixel registration based on intensity.
1998,
Pubmed
Verkade,
Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing.
2008,
Pubmed
van Rijnsoever,
Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections.
2008,
Pubmed