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XB-ART-48417
Methods Mol Biol 2014 Jan 01;1117:259-71. doi: 10.1007/978-1-62703-776-1_12.
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Electron microscopy of microtubule cytoskeleton assembly in vitro.

Coughlin M , Groen AC , Mitchison TJ .


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Cell-free cytoplasm isolated from meiotic Xenopus egg extracts reconstitutes microtubule phenomena in vitro. These crude extracts assemble bipolar meiotic spindles and are readily fractionated for biochemical assays, providing a good tool to dissect molecular mechanism. We developed techniques for immunoelectron microscopy of microtubule structures assembled in perfusion chambers and in solution.

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???displayArticle.link??? Methods Mol Biol
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