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Nucleic Acids Res
2003 Sep 01;3117:5039-47. doi: 10.1093/nar/gkg717.
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Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.
Garstka HL
,
Schmitt WE
,
Schultz J
,
Sogl B
,
Silakowski B
,
Pérez-Martos A
,
Montoya J
,
Wiesner RJ
.
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Mitochondrial transcription factor A (TFAM) has been shown to stimulate transcription from mitochondrial DNA promoters in vitro. In order to determine whether changes in TFAM levels also regulate RNA synthesis in situ, recombinant human precursor proteins were imported into the matrix of rat liver mitochondria. After uptake of wt-TFAM, incorporation of [alpha-32P]UTP into mitochondrial mRNAs as well as rRNAs was increased 2-fold (P < 0.05), whereas import of truncated TFAM lacking 25 amino acids at the C-terminus had no effect. Import of wt-TFAM into liver mitochondria from hypothyroid rats stimulated RNA synthesis up to 4-fold. We conclude that the rate of transcription is submaximal in freshly isolated rat liver mitochondria and that increasing intra-mitochondrial TFAM levels is sufficient for stimulation. The low transcription rate associated with the hypothyroid state observed in vivo as well as in organello seems to be a result of low TFAM levels, which can be recovered by treating animals with T3 in vivo or by importing TFAM in organello. Thus, this protein meets the criteria for being a key factor in regulating mitochondrial gene expression in vivo.
Adhihetty,
Plasticity of skeletal muscle mitochondria in response to contractile activity.
2003, Pubmed
Adhihetty,
Plasticity of skeletal muscle mitochondria in response to contractile activity.
2003,
Pubmed
Alam,
Human mitochondrial DNA is packaged with TFAM.
2003,
Pubmed
,
Xenbase
Antoshechkin,
Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA.
1995,
Pubmed
,
Xenbase
Attardi,
Biogenesis of mitochondria.
1988,
Pubmed
Barsano,
The effect of thyroid hormone on in vitro rat liver mitochondrial RNA synthesis.
1977,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cantatore,
Identification by in Organello footprinting of protein contact sites and of single-stranded DNA sequences in the regulatory region of rat mitochondrial DNA. Protein binding sites and single-stranded DNA regions in isolated rat liver mitochondria.
1995,
Pubmed
Cantatore,
Synthesis and turnover rates of four rat liver mitochondrial RNA species.
1987,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Clayton,
Vertebrate mitochondrial DNA-a circle of surprises.
2000,
Pubmed
Cornwall,
Differential expression of the mouse mitochondrial genes and the mitochondrial RNA-processing endoribonuclease RNA by androgens.
1992,
Pubmed
Dairaghi,
Human mitochondrial transcription factor A and promoter spacing integrity are required for transcription initiation.
1995,
Pubmed
Dairaghi,
Addition of a 29 residue carboxyl-terminal tail converts a simple HMG box-containing protein into a transcriptional activator.
1995,
Pubmed
Diffley,
DNA binding properties of an HMG1-related protein from yeast mitochondria.
1992,
Pubmed
Dirmeier,
Exposure of yeast cells to anoxia induces transient oxidative stress. Implications for the induction of hypoxic genes.
2002,
Pubmed
Enríquez,
Direct regulation of mitochondrial RNA synthesis by thyroid hormone.
1999,
Pubmed
Enríquez,
The synthesis of mRNA in isolated mitochondria can be maintained for several hours and is inhibited by high levels of ATP.
1996,
Pubmed
Enríquez,
In organello RNA synthesis system from mammalian liver and brain.
1996,
Pubmed
Falkenberg,
Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA.
2002,
Pubmed
Fernández-Silva,
Replication and transcription of mammalian mitochondrial DNA.
2003,
Pubmed
Fisher,
Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elements.
1987,
Pubmed
Fisher,
Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1.
1989,
Pubmed
Fisher,
A rapid, efficient method for purifying DNA-binding proteins. Denaturation-renaturation chromatography of human and yeast mitochondrial extracts.
1991,
Pubmed
Gadaleta,
Effects of triiodothyronine on rat-liver mitochondrial transcription process.
1975,
Pubmed
Gagnon,
Correlations between a nuclear and a mitochondrial mRNA of cytochrome c oxidase subunits, enzymatic activity and total mRNA content, in rat tissues.
1991,
Pubmed
Garstka,
Stoichiometry of mitochondrial transcripts and regulation of gene expression by mitochondrial transcription factor A.
1994,
Pubmed
,
Xenbase
Gensler,
Mechanism of mammalian mitochondrial DNA replication: import of mitochondrial transcription factor A into isolated mitochondria stimulates 7S DNA synthesis.
2001,
Pubmed
Ghivizzani,
In organello footprint analysis of human mitochondrial DNA: human mitochondrial transcription factor A interactions at the origin of replication.
1994,
Pubmed
,
Xenbase
Ghivizzani,
In organello footprinting. Analysis of protein binding at regulatory regions in bovine mitochondrial DNA.
1993,
Pubmed
Kanazawa,
Reduced activity of mtTFA decreases the transcription in mitochondria isolated from diabetic rat heart.
2002,
Pubmed
Klingenspor,
Biogenesis of thermogenic mitochondria in brown adipose tissue of Djungarian hamsters during cold adaptation.
1996,
Pubmed
Kuhn,
Functional differences between the two splice variants of the nucleolar transcription factor UBF: the second HMG box determines specificity of DNA binding and transcriptional activity.
1994,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Larsson,
Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice.
1998,
Pubmed
McCulloch,
A human mitochondrial transcription factor is related to RNA adenine methyltransferases and binds S-adenosylmethionine.
2002,
Pubmed
Micol,
Functional analysis of in vivo and in organello footprinting of HeLa cell mitochondrial DNA in relationship to ATP and ethidium bromide effects on transcription.
1997,
Pubmed
Montoya,
The pattern of transcription of the human mitochondrial rRNA genes reveals two overlapping transcription units.
1983,
Pubmed
Montoya,
Regulation of mitochondrial transcription by mitochondrial transcription factor A.
1997,
Pubmed
,
Xenbase
Nelson,
Thyroid hormone regulation of mitochondrial function. Comments on the mechanism of signal transduction.
1990,
Pubmed
Parisi,
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
1991,
Pubmed
Parisi,
A human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial HMG-box protein both in vivo and in vitro.
1993,
Pubmed
Rodríguez-Peña,
Thyroid hormone increases transcription of GA-binding protein/nuclear respiratory factor-2 alpha-subunit in rat liver.
2002,
Pubmed
Seidel-Rogol,
Modulation of mitochondrial transcription in response to mtDNA depletion and repletion in HeLa cells.
2002,
Pubmed
Shadel,
Mitochondrial DNA maintenance in vertebrates.
1997,
Pubmed
Shen,
Developmentally-regulated packaging of mitochondrial DNA by the HMG-box protein mtTFA during Xenopus oogenesis.
2001,
Pubmed
,
Xenbase
Spelbrink,
Human mitochondrial DNA deletions associated with mutations in the gene encoding Twinkle, a phage T7 gene 4-like protein localized in mitochondria.
2001,
Pubmed
Takamatsu,
Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein.
2002,
Pubmed
,
Xenbase
Tominaga,
Upstream region of a genomic gene for human mitochondrial transcription factor 1.
1992,
Pubmed
Van Itallie,
Dexamethasone treatment increases mitochondrial RNA synthesis in a rat hepatoma cell line.
1992,
Pubmed
Weber,
Glucocorticoid hormone stimulates mitochondrial biogenesis specifically in skeletal muscle.
2002,
Pubmed
Wiesner,
Regulation by thyroid hormone of nuclear and mitochondrial genes encoding subunits of cytochrome-c oxidase in rat liver and skeletal muscle.
1992,
Pubmed
Wredenberg,
Increased mitochondrial mass in mitochondrial myopathy mice.
2002,
Pubmed
Wrutniak,
A 43-kDa protein related to c-Erb A alpha 1 is located in the mitochondrial matrix of rat liver.
1995,
Pubmed