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Structural model of the anion exchanger 1 (SLC4A1) and identification of transmembrane segments forming the transport site.
Barneaud-Rocca D
,
Etchebest C
,
Guizouarn H
.
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The anion exchanger 1 (AE1), a member of bicarbonate transporter family SLC4, mediates an electroneutral chloride/bicarbonate exchange in physiological conditions. However, some point mutations in AE1 membrane-spanning domain convert the electroneutral anion exchanger into a Na(+) and K(+) conductance or induce a cation leak in a still functional anion exchanger. The molecular determinants that govern ion movement through this transporter are still unknown. The present study was intended to identify the ion translocation pathway within AE1. In the absence of a resolutive three-dimensional structure of AE1 membrane-spanning domain, in silico modeling combined with site-directed mutagenesis experiments was done. A structural model of AE1 membrane-spanning domain is proposed, and this model is based on the structure of a uracil-proton symporter. This model was used to design cysteine-scanning mutagenesis on transmembrane (TM) segments 3 and 5. By measuring AE1 anion exchange activity or cation leak, it is proposed that there is a unique transport site comprising TM3-5 and TM8 that should function as an anion exchanger and a cation leak.
Abramson,
Structure and function of Na(+)-symporters with inverted repeats.
2009, Pubmed
Abramson,
Structure and function of Na(+)-symporters with inverted repeats.
2009,
Pubmed
Barneaud-Rocca,
Dual transport properties of anion exchanger 1: the same transmembrane segment is involved in anion exchange and in a cation leak.
2011,
Pubmed
,
Xenbase
Benkert,
QMEAN server for protein model quality estimation.
2009,
Pubmed
Bruce,
Monovalent cation leaks in human red cells caused by single amino-acid substitutions in the transport domain of the band 3 chloride-bicarbonate exchanger, AE1.
2005,
Pubmed
,
Xenbase
Böhm,
Towards the localization of the essential arginine residues in the band 3 protein of human red blood cell membranes.
1996,
Pubmed
Chu,
Mapping of glycolytic enzyme-binding sites on human erythrocyte band 3.
2006,
Pubmed
Chu,
Band 3 Edmonton I, a novel mutant of the anion exchanger 1 causing spherocytosis and distal renal tubular acidosis.
2010,
Pubmed
,
Xenbase
Dutzler,
X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity.
2002,
Pubmed
Ellory,
Review. Leaky Cl--HCO3- exchangers: cation fluxes via modified AE1.
2009,
Pubmed
Faham,
The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport.
2008,
Pubmed
Fiévet,
Expression of band 3 anion exchanger induces chloride current and taurine transport: structure-function analysis.
1995,
Pubmed
,
Xenbase
Fiévet,
Transport of uncharged organic solutes in Xenopus oocytes expressing red cell anion exchangers (AE1s).
1998,
Pubmed
,
Xenbase
Fujinaga,
Topology of the membrane domain of human erythrocyte anion exchange protein, AE1.
1999,
Pubmed
Garcia-Romeu,
A role for the anion exchanger AE1 (band 3 protein) in cell volume regulation.
1996,
Pubmed
Groves,
Topology studies with biosynthetic fragments identify interacting transmembrane regions of the human red-cell anion exchanger (band 3; AE1).
1999,
Pubmed
,
Xenbase
Groves,
Structural model for the organization of the transmembrane spans of the human red-cell anion exchanger (band 3; AE1).
1999,
Pubmed
,
Xenbase
Guizouarn,
Multiple transport functions of a red blood cell anion exchanger, tAE1: its role in cell volume regulation.
2001,
Pubmed
,
Xenbase
Guizouarn,
Point mutations involved in red cell stomatocytosis convert the electroneutral anion exchanger 1 to a nonselective cation conductance.
2007,
Pubmed
,
Xenbase
Guizouarn,
South-east Asian ovalocytosis and the cryohydrocytosis form of hereditary stomatocytosis show virtually indistinguishable cation permeability defects.
2011,
Pubmed
,
Xenbase
Hirai,
Topology models of anion exchanger 1 that incorporate the anti-parallel V-shaped motifs found in the EM structure.
2011,
Pubmed
Iolascon,
A novel erythroid anion exchange variant (Gly796Arg) of hereditary stomatocytosis associated with dyserythropoiesis.
2009,
Pubmed
,
Xenbase
Jennings,
Proton fluxes associated with erythrocyte membrane anion exchange.
1976,
Pubmed
Jennings,
Anion-proton cotransport through the human red blood cell band 3 protein. Role of glutamate 681.
1992,
Pubmed
Kanki,
The tenth membrane region of band 3 is initially exposed to the luminal side of the endoplasmic reticulum and then integrated into a partially folded band 3 intermediate.
2002,
Pubmed
Krishnamurthy,
Unlocking the molecular secrets of sodium-coupled transporters.
2009,
Pubmed
Kuma,
Topology of the anion exchange protein AE1: the controversial sidedness of lysine 743.
2002,
Pubmed
Lindenthal,
Monomeric erythrocyte band 3 protein transports anions.
1991,
Pubmed
Lomize,
OPM database and PPM web server: resources for positioning of proteins in membranes.
2012,
Pubmed
Lu,
Structure and mechanism of the uracil transporter UraA.
2011,
Pubmed
Parker,
A conductive pathway generated from fragments of the human red cell anion exchanger AE1.
2007,
Pubmed
,
Xenbase
Passow,
Anion transport across the red blood cell membrane and the conformation of the protein in Band 3.
1980,
Pubmed
Paw,
Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency.
2003,
Pubmed
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Picollo,
Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
2009,
Pubmed
Popov,
Mapping the ends of transmembrane segments in a polytopic membrane protein. Scanning N-glycosylation mutagenesis of extracytosolic loops in the anion exchanger, band 3.
1997,
Pubmed
Pucéat,
Identification of band 3-like proteins and Cl-/HCO3- exchange in isolated cardiomyocytes.
1995,
Pubmed
Ray,
Improved model quality assessment using ProQ2.
2012,
Pubmed
Stewart,
Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis.
2011,
Pubmed
,
Xenbase
Tang,
Topology of the region surrounding Glu681 of human AE1 protein, the erythrocyte anion exchanger.
1998,
Pubmed
Tang,
Identification of residues lining the translocation pore of human AE1, plasma membrane anion exchange protein.
1999,
Pubmed
Taylor,
Cysteine-directed cross-linking localizes regions of the human erythrocyte anion-exchange protein (AE1) relative to the dimeric interface.
2001,
Pubmed
Wainwright,
The membrane domain of the human erythrocyte anion transport protein. Epitope mapping of a monoclonal antibody defines the location of a cytoplasmic loop near the C-terminus of the protein.
1990,
Pubmed
Walsh,
Cation transport activity of anion exchanger 1 mutations found in inherited distal renal tubular acidosis.
2008,
Pubmed
,
Xenbase
Walsh,
Southeast Asian AE1 associated renal tubular acidosis: cation leak is a class effect.
2009,
Pubmed
,
Xenbase
Weyand,
Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.
2008,
Pubmed
Yamaguchi,
Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals.
2010,
Pubmed
Zaki,
Chemical labelling of arginyl-residues involved in anion transport mediated by human band 3 protein and some aspects of its location in the peptide chain.
1996,
Pubmed
Zhu,
Novel topology in C-terminal region of the human plasma membrane anion exchanger, AE1.
2003,
Pubmed