Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Organization and adhesive properties of the hyaluronan pericellular coat of chondrocytes and epithelial cells.
Cohen M
,
Klein E
,
Geiger B
,
Addadi L
.
???displayArticle.abstract???
Hyaluronan is a megadalton glycosaminoglycan composed of repeating units of D-N-acetylglucosamine-beta-D-Glucuronic acid. It is known to form a highly hydrated pericellular coat around chondrocytes, fibrosarcoma, and smooth muscle cells. Using environmental scanning electron microscopy we detected fully hydrated hyaluronan pericellular coats around rat chondrocytes (RCJ-P) and epithelial cells (A6). Hyaluronan mediates early adhesion of both chondrocytes and A6 cells to glass surfaces. We show that chondrocytes in suspension establish early "soft contacts" with the substrate through a thick, hyaluronidase-sensitive coat (4.4 +/- 0.7 microm). Freshly-attached cells drift under shear stress, leaving hyaluronan "footprints" on the surface. This suggests that chondrocytes are surrounded by a multilayer of entangled hyaluronan molecules. In contrast, A6 cells have a 2.2 +/- 0.4- microm-thick hyaluronidase-sensitive coat, do not drift under shear stress, and remain firmly anchored to the surface. We consider the possibility that in A6 cells single hyaluronan molecules, spanning the whole thickness of the pericellular coat, mediate these tight contacts.
Adams,
Cell-matrix contact structures.
2001,
Pubmed
Bajorath,
Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions.
2000,
Pubmed
Bard,
Morphology of hyaluronidase-sensitive cell coats as seen in the SEM after freeze-drying.
1983,
Pubmed
Borland,
Forms and functions of CD44.
1998,
Pubmed
Cowman,
Tapping mode atomic force microscopy of hyaluronan: extended and intramolecularly interacting chains.
1998,
Pubmed
Evanko,
Formation of hyaluronan- and versican-rich pericellular matrix is required for proliferation and migration of vascular smooth muscle cells.
1999,
Pubmed
Geiger,
Transmembrane crosstalk between the extracellular matrix--cytoskeleton crosstalk.
2001,
Pubmed
Gribbon,
The analysis of intermolecular interactions in concentrated hyaluronan solutions suggest no evidence for chain-chain association.
2000,
Pubmed
Hadler,
Ultrastructure of a hyaluronic acid matrix.
1982,
Pubmed
Hanein,
Selective interactions of cells with crystal surfaces. Implications for the mechanism of cell adhesion.
1993,
Pubmed
,
Xenbase
Hanein,
Differential adhesion of cells to enantiomorphous crystal surfaces.
1994,
Pubmed
,
Xenbase
Israelachvili,
Role of hydration and water structure in biological and colloidal interactions.
1996,
Pubmed
Jacoboni,
Hyaluronic acid by atomic force microscopy.
1999,
Pubmed
Kiani,
Structure and function of aggrecan.
2002,
Pubmed
Knudson,
Cartilage proteoglycans.
2001,
Pubmed
Knudson,
CD44-mediated uptake and degradation of hyaluronan.
2002,
Pubmed
Krause,
Rheology of sodium hyaluronate under physiological conditions.
2001,
Pubmed
Laurent,
Biochemistry of hyaluronan.
1987,
Pubmed
,
Xenbase
Lee,
Hyaluronan: a multifunctional, megaDalton, stealth molecule.
2000,
Pubmed
,
Xenbase
Lee,
The dynamic structure of the pericellular matrix on living cells.
1993,
Pubmed
Martin,
Integrin connections map: to infinity and beyond.
2002,
Pubmed
McBride,
Hyaluronidase-sensitive halos around adherent cells. Their role in blocking lymphocyte-mediated cytolysis.
1979,
Pubmed
Scott,
Hyaluronan forms specific stable tertiary structures in aqueous solution: a 13C NMR study.
1999,
Pubmed
Scott,
Biological properties of hyaluronan in aqueous solution are controlled and sequestered by reversible tertiary structures, defined by NMR spectroscopy.
2002,
Pubmed
Scott,
Supramolecular organization of extracellular matrix glycosaminoglycans, in vitro and in the tissues.
1992,
Pubmed
Tadmor,
Thin film rheology and lubricity of hyaluronic acid solutions at a normal physiological concentration.
2002,
Pubmed
Toole,
Hyaluronan in morphogenesis.
2001,
Pubmed
Weigel,
Hyaluronan synthases.
1997,
Pubmed
,
Xenbase
Zamir,
Molecular diversity of cell-matrix adhesions.
1999,
Pubmed
Zimmerman,
Initial stages of cell-matrix adhesion can be mediated and modulated by cell-surface hyaluronan.
2002,
Pubmed
,
Xenbase