Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2011 May 06;28618:15625-9. doi: 10.1074/jbc.C111.220715.
Show Gene links
Show Anatomy links
Structure and histone binding properties of the Vps75-Rtt109 chaperone-lysine acetyltransferase complex.
Su D
,
Hu Q
,
Zhou H
,
Thompson JR
,
Xu RM
,
Zhang Z
,
Mer G
.
???displayArticle.abstract???
The histone chaperone Vps75 presents the remarkable property of stimulating the Rtt109-dependent acetylation of several histone H3 lysine residues within (H3-H4)(2) tetramers. To investigate this activation mechanism, we determined x-ray structures of full-length Vps75 in complex with full-length Rtt109 in two crystal forms. Both structures show similar asymmetric assemblies of a Vps75 dimer bound to an Rtt109 monomer. In the Vps75-Rtt109 complexes, the catalytic site of Rtt109 is confined to an enclosed space that can accommodate the N-terminal tail of histone H3 in (H3-H4)(2). Investigation of Vps75-Rtt109-(H3-H4)(2) and Vps75-(H3-H4)(2) complexes by NMR spectroscopy-probed hydrogen/deuterium exchange suggests that Vps75 guides histone H3 in the catalytic enclosure. These findings clarify the basis for the enhanced acetylation of histone H3 tail residues by Vps75-Rtt109.
Adams,
Recent developments in the PHENIX software for automated crystallographic structure determination.
2004, Pubmed
Adams,
Recent developments in the PHENIX software for automated crystallographic structure determination.
2004,
Pubmed
Adams,
PHENIX: building new software for automated crystallographic structure determination.
2002,
Pubmed
Albaugh,
Kinetic mechanism of the Rtt109-Vps75 histone acetyltransferase-chaperone complex.
2010,
Pubmed
,
Xenbase
Baker,
Electrostatics of nanosystems: application to microtubules and the ribosome.
2001,
Pubmed
Berndsen,
Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75.
2008,
Pubmed
,
Xenbase
Burgess,
A role for Gcn5 in replication-coupled nucleosome assembly.
2010,
Pubmed
Chen,
MolProbity: all-atom structure validation for macromolecular crystallography.
2010,
Pubmed
Driscoll,
Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56.
2007,
Pubmed
Dyson,
Hydrogen-deuterium exchange strategy for delineation of contact sites in protein complexes.
2008,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
English,
Structural basis for the histone chaperone activity of Asf1.
2006,
Pubmed
,
Xenbase
Fillingham,
Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109.
2008,
Pubmed
Han,
Acetylation of lysine 56 of histone H3 catalyzed by RTT109 and regulated by ASF1 is required for replisome integrity.
2007,
Pubmed
Han,
The Rtt109-Vps75 histone acetyltransferase complex acetylates non-nucleosomal histone H3.
2007,
Pubmed
,
Xenbase
Han,
Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication.
2007,
Pubmed
Kato,
Characterization of the N-terminal tail domain of histone H3 in condensed nucleosome arrays by hydrogen exchange and NMR.
2009,
Pubmed
Kolonko,
Catalytic activation of histone acetyltransferase Rtt109 by a histone chaperone.
2010,
Pubmed
Lin,
Structural insights into histone H3 lysine 56 acetylation by Rtt109.
2008,
Pubmed
McCoy,
Phaser crystallographic software.
2007,
Pubmed
Minor,
HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes.
2006,
Pubmed
Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed
Natsume,
Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4.
2007,
Pubmed
,
Xenbase
Park,
Histone chaperone specificity in Rtt109 activation.
2008,
Pubmed
Ransom,
Chaperoning histones during DNA replication and repair.
2010,
Pubmed
Schneider,
Rtt109 is required for proper H3K56 acetylation: a chromatin mark associated with the elongating RNA polymerase II.
2006,
Pubmed
Stavropoulos,
Molecular basis for the autoregulation of the protein acetyl transferase Rtt109.
2008,
Pubmed
Tang,
Structure of Vps75 and implications for histone chaperone function.
2008,
Pubmed
Tsubota,
Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes.
2007,
Pubmed
