Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2013 Jan 01;331:159-69. doi: 10.1128/MCB.01131-12.
Show Gene links
Show Anatomy links
Piccolo NuA4-catalyzed acetylation of nucleosomal histones: critical roles of an Esa1 Tudor/chromo barrel loop and an Epl1 enhancer of polycomb A (EPcA) basic region.
Huang J
,
Tan S
.
???displayArticle.abstract???
Piccolo NuA4 is an essential yeast histone acetyltransferase (HAT) complex that targets histones H4 and H2A in nucleosome substrates. While Piccolo NuA4's catalytic subunit Esa1 alone is unable to acetylate nucleosomal histones, its accessory subunits, Yng2 and Epl1, enable Esa1 to bind to and to act on nucleosomes. We previously determined that the Tudor domain of Esa1 and the EPcA homology domain of Epl1 play critical roles in Piccolo NuA4's ability to act on the nucleosome. In this work, we pinpoint a loop within the Esa1 Tudor domain and a short basic region at the N terminus of the Epl1 EPcA domain as necessary for this nucleosomal HAT activity. We also show that this Esa1 Tudor domain loop region is positioned close to nucleosomal DNA and that the Epl1 EPcA basic region is in proximity to the N-terminal histone H2A tail, the globular region of histone H4, and also to nucleosomal DNA when Piccolo NuA4 interacts with the nucleosome. Since neither region identified is required for Piccolo NuA4 to bind to nucleosomes and yet both are needed to acetylate nucleosomes, these regions may function after the enzyme binds nucleosomes to disengage substrate histone tails from nucleosomal DNA.
Akhtar,
Chromodomains are protein-RNA interaction modules.
2000, Pubmed
Akhtar,
Chromodomains are protein-RNA interaction modules.
2000,
Pubmed
Balasubramanian,
Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylation.
2002,
Pubmed
Bannister,
Regulation of chromatin by histone modifications.
2011,
Pubmed
Barrios,
Expression and purification of recombinant yeast Ada2/Ada3/Gcn5 and Piccolo NuA4 histone acetyltransferase complexes.
2007,
Pubmed
Berndsen,
Nucleosome recognition by the Piccolo NuA4 histone acetyltransferase complex.
2007,
Pubmed
,
Xenbase
Boudreault,
Yeast enhancer of polycomb defines global Esa1-dependent acetylation of chromatin.
2003,
Pubmed
Brownell,
Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.
1996,
Pubmed
Chahal,
The SufBCD Fe-S scaffold complex interacts with SufA for Fe-S cluster transfer.
2009,
Pubmed
Chin,
Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli.
2002,
Pubmed
Chittuluru,
Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes.
2011,
Pubmed
Clements,
Structural basis for histone and phosphohistone binding by the GCN5 histone acetyltransferase.
2003,
Pubmed
Cobb,
Identification of the eosinyl-5-maleimide reaction site on the human erythrocyte anion-exchange protein: overlap with the reaction sites of other chemical probes.
1990,
Pubmed
Conrad,
The MOF chromobarrel domain controls genome-wide H4K16 acetylation and spreading of the MSL complex.
2012,
Pubmed
Decker,
Catalytic-site mutations in the MYST family histone Acetyltransferase Esa1.
2008,
Pubmed
Dou,
Regulation of MLL1 H3K4 methyltransferase activity by its core components.
2006,
Pubmed
Eissenberg,
Structural biology of the chromodomain: form and function.
2012,
Pubmed
Gao,
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA.
1998,
Pubmed
Gietz,
Improved method for high efficiency transformation of intact yeast cells.
1992,
Pubmed
Grant,
Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
1997,
Pubmed
Layer,
SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly.
2007,
Pubmed
Lee,
An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation.
2005,
Pubmed
Lee,
The language of histone crosstalk.
2010,
Pubmed
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Makde,
Structure of RCC1 chromatin factor bound to the nucleosome core particle.
2010,
Pubmed
,
Xenbase
Marmorstein,
Structure of histone acetyltransferases.
2001,
Pubmed
Maurer-Stroh,
The Tudor domain 'Royal Family': Tudor, plant Agenet, Chromo, PWWP and MBT domains.
2003,
Pubmed
Müller,
Histone methyltransferase activity of a Drosophila Polycomb group repressor complex.
2002,
Pubmed
Nielsen,
Structure of the chromo barrel domain from the MOF acetyltransferase.
2005,
Pubmed
Padrick,
Arp2/3 complex is bound and activated by two WASP proteins.
2011,
Pubmed
Patel,
A novel non-SET domain multi-subunit methyltransferase required for sequential nucleosomal histone H3 methylation by the mixed lineage leukemia protein-1 (MLL1) core complex.
2011,
Pubmed
Rando,
Chromatin and transcription in yeast.
2012,
Pubmed
Rojas,
Structure of Tetrahymena GCN5 bound to coenzyme A and a histone H3 peptide.
1999,
Pubmed
Ryu,
Efficient incorporation of unnatural amino acids into proteins in Escherichia coli.
2006,
Pubmed
Selleck,
The Saccharomyces cerevisiae Piccolo NuA4 histone acetyltransferase complex requires the Enhancer of Polycomb A domain and chromodomain to acetylate nucleosomes.
2005,
Pubmed
Sharma,
Histone deacetylases RPD3 and HOS2 regulate the transcriptional activation of DNA damage-inducible genes.
2007,
Pubmed
Shimojo,
Novel structural and functional mode of a knot essential for RNA binding activity of the Esa1 presumed chromodomain.
2008,
Pubmed
Smyth,
Reactions of N-ethylmaleimide with peptides and amino acids.
1964,
Pubmed
Tan,
The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.
2005,
Pubmed
Trievel,
Crystal structure and mechanism of histone acetylation of the yeast GCN5 transcriptional coactivator.
1999,
Pubmed
White,
Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions.
2001,
Pubmed
,
Xenbase
Yan,
Crystal structure of yeast Esa1 suggests a unified mechanism for catalysis and substrate binding by histone acetyltransferases.
2000,
Pubmed