XB-ART-46761
Cold Spring Harb Protoc
2013 Mar 01;20133:. doi: 10.1101/pdb.top066308.
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The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.
Lin-Moshier Y
,
Marchant JS
.
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In the four decades since the Xenopus oocyte was first demonstrated to have the capacity to translate exogenous mRNAs, this system has been exploited for many different experimental purposes. Typically, the oocyte is used either as a "biological test tube" for heterologous expression of proteins without any particular cell biological insight or, alternatively, it is used for applications where cell biology is paramount, such as investigations of the cellular adaptations that power early development. In this article, we discuss the utility of the Xenopus oocyte for studying Ca(2+) signaling in both these contexts.
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References [+] :
Agboh,
Functional characterization of a P2X receptor from Schistosoma mansoni.
2004, Pubmed,
Xenbase
Agboh, Functional characterization of a P2X receptor from Schistosoma mansoni. 2004, Pubmed , Xenbase
Anderson, Patterns of synthesis and accumulation of heterogeneous RNA in lampbrush stage oocytes of Xenopus laevis (Daudin). 1978, Pubmed , Xenbase
Anderson, Sequence organization of the poly(A) RNA synthesized and accumulated in lampbrush chromosome stage Xenopus laevis oocytes. 1982, Pubmed , Xenbase
Antoine, The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. 2007, Pubmed , Xenbase
Au, Microinjection of Xenopus laevis oocytes as a system for studying nuclear transport of viruses. 2010, Pubmed , Xenbase
Barnard, Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. 1982, Pubmed , Xenbase
Beene, Unnatural amino acid mutagenesis in mapping ion channel function. 2003, Pubmed , Xenbase
Bianchi, The neurotoxic MEC-4(d) DEG/ENaC sodium channel conducts calcium: implications for necrosis initiation. 2004, Pubmed , Xenbase
Bocquet, A prokaryotic proton-gated ion channel from the nicotinic acetylcholine receptor family. 2007, Pubmed , Xenbase
Boulter, Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase. 2006, Pubmed
Boulware, Nuclear pore disassembly from endoplasmic reticulum membranes promotes Ca2+ signalling competency. 2008, Pubmed , Xenbase
Boulware, IP3 receptor activity is differentially regulated in endoplasmic reticulum subdomains during oocyte maturation. 2005, Pubmed , Xenbase
Bowes, Xenbase: gene expression and improved integration. 2010, Pubmed , Xenbase
Brailoiu, An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organelles. 2010, Pubmed
Brown, A tribute to the Xenopus laevis oocyte and egg. 2004, Pubmed , Xenbase
Brown, Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. 1968, Pubmed
Camacho, Increased frequency of calcium waves in Xenopus laevis oocytes that express a calcium-ATPase. 1993, Pubmed , Xenbase
Chase, Biogenesis of mitochondria during Xenopus laevis development. 1972, Pubmed , Xenbase
Choi, Identification and characterization of a novel bacterial ATP-sensitive K+ channel. 2010, Pubmed , Xenbase
Cordes, High content of a nuclear pore complex protein in cytoplasmic annulate lamellae of Xenopus oocytes. 1995, Pubmed , Xenbase
Demuro, Imaging single-channel calcium microdomains. 2006, Pubmed , Xenbase
Dumont, Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. 1972, Pubmed , Xenbase
Eckstein-Ludwig, Artemisinins target the SERCA of Plasmodium falciparum. 2003, Pubmed , Xenbase
Fletcher, TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions. 2007, Pubmed , Xenbase
Fujioka, Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. 2006, Pubmed
Geib, A novel Xenopus oocyte expression system based on cytoplasmic coinjection of T7-driven plasmids and purified T7-RNA polymerase. 2001, Pubmed , Xenbase
Girard, Acceleration of intracellular calcium waves in Xenopus oocytes by calcium influx. 1993, Pubmed , Xenbase
Gurdon, Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. 1971, Pubmed , Xenbase
Hallberg, Ribosomal protein synthesis in Xenopus laevis oocytes. 1975, Pubmed , Xenbase
Halley-Stott, Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes - a method for quantitative transcriptional reprogramming. 2010, Pubmed , Xenbase
Harland, Xenopus research: metamorphosed by genetics and genomics. 2011, Pubmed , Xenbase
Izumi-Nakaseko, DHP-insensitive L-type-like Ca channel of ascidian acquires sensitivity to DHP with single amino acid change in domain III P-region. 2003, Pubmed , Xenbase
Jeziorski, Cloning and functional expression of a voltage-gated calcium channel alpha1 subunit from jellyfish. 1998, Pubmed , Xenbase
Jouaville, Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes. 1995, Pubmed , Xenbase
Kimura, Functional identification of a cloned squid presynaptic voltage-dependent calcium channel. 2002, Pubmed , Xenbase
Kume, Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos. 1997, Pubmed , Xenbase
Lan, A rice high-affinity potassium transporter (HKT) conceals a calcium-permeable cation channel. 2010, Pubmed
Le Cahérec, Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection: functional characterization of a novel aquaporin. 1996, Pubmed , Xenbase
Lechleiter, Spiral calcium wave propagation and annihilation in Xenopus laevis oocytes. 1991, Pubmed , Xenbase
Lechleiter, Subcellular patterns of calcium release determined by G protein-specific residues of muscarinic receptors. 1991, Pubmed , Xenbase
Lee, Subcellular in vivo 1H MR spectroscopy of Xenopus laevis oocytes. 2006, Pubmed , Xenbase
Legros, Organization and dynamics of human mitochondrial DNA. 2004, Pubmed
Lesage, A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties. 1996, Pubmed , Xenbase
Lin-Moshier, Nuclear microinjection to assess how heterologously expressed proteins impact Ca2+ signals in Xenopus oocytes. 2013, Pubmed , Xenbase
Lin-Moshier, A rapid Western blotting protocol for the Xenopus oocyte. 2013, Pubmed , Xenbase
Ludlow, Functional characterization of intracellular Dictyostelium discoideum P2X receptors. 2009, Pubmed , Xenbase
Lénárt, Nuclear envelope dynamics in oocytes: from germinal vesicle breakdown to mitosis. 2003, Pubmed
Lübbert, cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes. 1987, Pubmed , Xenbase
Mak, Single-channel inositol 1,4,5-trisphosphate receptor currents revealed by patch clamp of isolated Xenopus oocyte nuclei. 1994, Pubmed , Xenbase
Maksaev, Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes. 2011, Pubmed , Xenbase
Marchant, Role of elementary Ca(2+) puffs in generating repetitive Ca(2+) oscillations. 2001, Pubmed , Xenbase
Marchant, Xenopus tropicalis oocytes as an advantageous model system for the study of intracellular Ca(2+) signalling. 2001, Pubmed , Xenbase
Marinos, The number of mitochondria in Xenopus laevis ovulated oocytes. 1985, Pubmed , Xenbase
Markovich, Expression cloning and radiotracer uptakes in Xenopus laevis oocytes. 2008, Pubmed , Xenbase
Miledi, Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes. 1983, Pubmed , Xenbase
Miledi, Chloride current induced by injection of calcium into Xenopus oocytes. 1984, Pubmed , Xenbase
Moar, Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection. 1971, Pubmed
Mosimann, A Trk/HKT-type K+ transporter from Trypanosoma brucei. 2010, Pubmed , Xenbase
Nagel, Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. 2003, Pubmed , Xenbase
Nielsen, Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes. 1982, Pubmed , Xenbase
Ottolia, Xenopus oocyte plasma membrane sheets for FRET analysis. 2007, Pubmed , Xenbase
Palma, Microtransplantation of membranes from cultured cells to Xenopus oocytes: a method to study neurotransmitter receptors embedded in native lipids. 2003, Pubmed , Xenbase
Papke, Working with OpusXpress: methods for high volume oocyte experiments. 2010, Pubmed , Xenbase
Parker, Elementary events of InsP3-induced Ca2+ liberation in Xenopus oocytes: hot spots, puffs and blips. 1996, Pubmed , Xenbase
Parker, Regenerative release of calcium from functionally discrete subcellular stores by inositol trisphosphate. 1991, Pubmed , Xenbase
Parys, Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes. 1992, Pubmed , Xenbase
Pearl, Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center. 2012, Pubmed , Xenbase
Peters, Use of Xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studies. 2006, Pubmed , Xenbase
Roy, Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana. 2008, Pubmed , Xenbase
Saneyoshi, The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos. 2002, Pubmed , Xenbase
Schroeder, Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. 2008, Pubmed , Xenbase
Sheu, Rapid report: a novel technique for quantitative measurement of free Ca2+ concentration in rat heart mitochondria. 1999, Pubmed , Xenbase
Sigel, Use of Xenopus oocytes for the functional expression of plasma membrane proteins. 1990, Pubmed , Xenbase
Sims, Analysis of single mammalian cells on-chip. 2007, Pubmed
Sindelka, Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography. 2010, Pubmed , Xenbase
Sobczak, Endogenous transport systems in the Xenopus laevis oocyte plasma membrane. 2010, Pubmed , Xenbase
Sonnleitner, Structural rearrangements in single ion channels detected optically in living cells. 2002, Pubmed , Xenbase
Soreq, Xenopus oocyte microinjection: from gene to protein. 1992, Pubmed , Xenbase
Steinman, Membrane flow during pinocytosis. A stereologic analysis. 1976, Pubmed
Tompkins-Macdonald, Expression of a poriferan potassium channel: insights into the evolution of ion channels in metazoans. 2009, Pubmed , Xenbase
Wall, An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes. 1985, Pubmed , Xenbase
Webb, Accumulation of mitochondrial DNA during oogenesis in Xenopus laevis. 1977, Pubmed , Xenbase
Wolf, Nuclear metabolism of ribosomal RNA in growing, methionine-limited, and ethionine-treated HeLa cells. 1977, Pubmed
Yu, Orai1 internalization and STIM1 clustering inhibition modulate SOCE inactivation during meiosis. 2009, Pubmed , Xenbase
Zampighi, Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes. 1999, Pubmed , Xenbase
Zhang, Molecular characterization and functional expression of the DSC1 channel. 2011, Pubmed , Xenbase
Zhang, The inositol 1,4,5-trisphosphate receptor (Itpr) gene family in Xenopus: identification of type 2 and type 3 inositol 1,4,5-trisphosphate receptor subtypes. 2007, Pubmed , Xenbase
Zhou, A voltage-gated calcium-selective channel encoded by a sodium channel-like gene. 2004, Pubmed , Xenbase