Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurosci
2012 Nov 14;3246:16285-95. doi: 10.1523/JNEUROSCI.2667-12.2012.
Show Gene links
Show Anatomy links
IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a G(o) signaling pathway.
Emtage L
,
Aziz-Zaman S
,
Padovan-Merhar O
,
Horvitz HR
,
Fang-Yen C
,
Ringstad N
.
???displayArticle.abstract???
To identify molecular mechanisms that function in G-protein signaling, we have performed molecular genetic studies of a simple behavior of the nematode Caenorhabditis elegans, egg laying, which is driven by a pair of serotonergic neurons, the hermaphrodite-specific neurons (HSNs). The activity of the HSNs is regulated by the G(o)-coupled receptor EGL-6, which mediates inhibition of the HSNs by neuropeptides. We report here that this inhibition requires one of three inwardly rectifying K(+) channels encoded by the C. elegans genome: IRK-1. Using ChannelRhodopsin-2-mediated stimulation of HSNs, we observed roles for egl-6 and irk-1 in regulating the excitability of HSNs. Although irk-1 is required for inhibition of HSNs by EGL-6 signaling, we found that other G(o) signaling pathways that inhibit HSNs involve irk-1 little or not at all. These findings suggest that the neuropeptide receptor EGL-6 regulates the potassium channel IRK-1 via a dedicated pool of G(o) not involved in other G(o)-mediated signaling. We conclude that G-protein-coupled receptors that signal through the same G-protein in the same cell might activate distinct effectors and that specific coupling of a G-protein-coupled receptor to its effectors can be determined by factors other than its associated G-proteins.
Bany,
Genetic and cellular basis for acetylcholine inhibition of Caenorhabditis elegans egg-laying behavior.
2003, Pubmed
Bany,
Genetic and cellular basis for acetylcholine inhibition of Caenorhabditis elegans egg-laying behavior.
2003,
Pubmed
Bargmann,
Neurobiology of the Caenorhabditis elegans genome.
1998,
Pubmed
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
Carnell,
The G-protein-coupled serotonin receptor SER-1 regulates egg laying and male mating behaviors in Caenorhabditis elegans.
2005,
Pubmed
Drenkard,
A simple procedure for the analysis of single nucleotide polymorphisms facilitates map-based cloning in Arabidopsis.
2000,
Pubmed
Fang-Yen,
Laser microsurgery in Caenorhabditis elegans.
2012,
Pubmed
Fowler,
Evidence for association of GABA(B) receptors with Kir3 channels and regulators of G protein signalling (RGS4) proteins.
2007,
Pubmed
Goodman,
Active currents regulate sensitivity and dynamic range in C. elegans neurons.
1998,
Pubmed
Guo,
Optical interrogation of neural circuits in Caenorhabditis elegans.
2009,
Pubmed
Hallem,
Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans.
2011,
Pubmed
Herlitze,
Modulation of Ca2+ channels by G-protein beta gamma subunits.
1996,
Pubmed
Hobson,
SER-7, a Caenorhabditis elegans 5-HT7-like receptor, is essential for the 5-HT stimulation of pharyngeal pumping and egg laying.
2006,
Pubmed
Huang,
Direct activation of inward rectifier potassium channels by PIP2 and its stabilization by Gbetagamma.
1998,
Pubmed
,
Xenbase
Jansen,
Reverse genetics by chemical mutagenesis in Caenorhabditis elegans.
1997,
Pubmed
Kim,
Expression and regulation of an FMRFamide-related neuropeptide gene family in Caenorhabditis elegans.
2004,
Pubmed
Koelle,
EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins.
1996,
Pubmed
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Labouèbe,
RGS2 modulates coupling between GABAB receptors and GIRK channels in dopamine neurons of the ventral tegmental area.
2007,
Pubmed
Lee,
Characterization of GAR-2, a novel G protein-linked acetylcholine receptor from Caenorhabditis elegans.
2000,
Pubmed
,
Xenbase
Lefkowitz,
Transduction of receptor signals by beta-arrestins.
2005,
Pubmed
Leifer,
Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans.
2011,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lindsay,
Optogenetic analysis of synaptic transmission in the central nervous system of the nematode Caenorhabditis elegans.
2011,
Pubmed
Lu,
Peptide neurotransmitters activate a cation channel complex of NALCN and UNC-80.
2009,
Pubmed
Luján,
New sites of action for GIRK and SK channels.
2009,
Pubmed
Lüscher,
G protein-coupled inwardly rectifying K+ channels (GIRKs) mediate postsynaptic but not presynaptic transmitter actions in hippocampal neurons.
1997,
Pubmed
Mellem,
Action potentials contribute to neuronal signaling in C. elegans.
2008,
Pubmed
Mello,
DNA transformation.
1995,
Pubmed
Mello,
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
1991,
Pubmed
Mendel,
Participation of the protein Go in multiple aspects of behavior in C. elegans.
1995,
Pubmed
Moresco,
Activation of EGL-47, a Galpha(o)-coupled receptor, inhibits function of hermaphrodite-specific motor neurons to regulate Caenorhabditis elegans egg-laying behavior.
2004,
Pubmed
Nagel,
Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses.
2005,
Pubmed
Nurrish,
Serotonin inhibition of synaptic transmission: Galpha(0) decreases the abundance of UNC-13 at release sites.
1999,
Pubmed
Ramot,
Bidirectional temperature-sensing by a single thermosensory neuron in C. elegans.
2008,
Pubmed
Reuveny,
Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits.
1994,
Pubmed
,
Xenbase
Ringstad,
FMRFamide neuropeptides and acetylcholine synergistically inhibit egg-laying by C. elegans.
2008,
Pubmed
Sbalzarini,
Feature point tracking and trajectory analysis for video imaging in cell biology.
2005,
Pubmed
Sieburth,
PKC-1 regulates secretion of neuropeptides.
2007,
Pubmed
Suh,
PIP2 is a necessary cofactor for ion channel function: how and why?
2008,
Pubmed
Ségalat,
Modulation of serotonin-controlled behaviors by Go in Caenorhabditis elegans.
1995,
Pubmed
Takigawa,
Phasic and tonic attenuation of EPSPs by inward rectifier K+ channels in rat hippocampal pyramidal cells.
2002,
Pubmed
Tanis,
Regulation of serotonin biosynthesis by the G proteins Galphao and Galphaq controls serotonin signaling in Caenorhabditis elegans.
2008,
Pubmed
Tanis,
The potassium chloride cotransporter KCC-2 coordinates development of inhibitory neurotransmission and synapse structure in Caenorhabditis elegans.
2009,
Pubmed
White,
The structure of the nervous system of the nematode Caenorhabditis elegans.
1986,
Pubmed
Whorton,
Crystal structure of the mammalian GIRK2 K+ channel and gating regulation by G proteins, PIP2, and sodium.
2011,
Pubmed
,
Xenbase
Zimmer,
Neurons detect increases and decreases in oxygen levels using distinct guanylate cyclases.
2009,
Pubmed