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J Membr Biol
2003 Aug 01;1943:165-76. doi: 10.1007/s00232-003-2033-9.
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Regulation of ion fluxes, cell volume and gap junctional coupling by cGMP in GFSHR-17 granulosa cells.
Ngezahayo A
,
Altmann B
,
Kolb HA
.
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Gap junctional communication between granulosa cells seems to play a crucial role for follicular growth and atresia. Application of the double whole-cell patch-clamp- and ratiometric fura-2-techniques allowed a simultaneous measurement of gap junctional conductance ( G(j)) and cytoplasmic concentration of free Ca(2+) ([Ca(2+)](i)) in a rat granulosa cell line GFSHR-17. The voltage-dependent gating of G(j) varied for different cell pairs. One population exhibited a bell-shape dependence of G(j) on transjunctional voltage, which was strikingly similar to that of Cx43/Cx43 homotypic gap junction channels expressed in pairs of oocytes of Xenopus laevis. Within 15-20 min, gap junctional uncoupling occurred spontaneously, which was preceded by a sustained increase of [Ca(2+)](i) and accompanied by shrinkage of cellular volume. These responses to the whole-cell configuration were avoided by absence of extracellular Ca(2+), blockage of K(+) efflux, or addition of 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) to the pipette solution. Even in the absence of extracellular Ca(2+) or blockage of K(+) efflux, formation of whole-cell configuration generated a Ca(2+) spike that could be suppressed by the presence of 8-Br-cGMP. We propose that intracellular cGMP regulates Ca(2+) release from intracellular Ca(2+) stores, which activates sustained Ca(2+) influx, K(+) efflux and cellular shrinkage. We discuss whether gap junctional conductance is directly affected by cGMP or by cellular shrinkage and whether gap junctional coupling and/or cell shrinkage is involved in the regulation of apoptotic/necrotic processes in granulosa cells.
Ackert,
Intercellular communication via connexin43 gap junctions is required for ovarian folliculogenesis in the mouse.
2001, Pubmed
Ackert,
Intercellular communication via connexin43 gap junctions is required for ovarian folliculogenesis in the mouse.
2001,
Pubmed
Banach,
Connexin43 gap junctions exhibit asymmetrical gating properties.
1996,
Pubmed
Bevans,
Isoform composition of connexin channels determines selectivity among second messengers and uncharged molecules.
1998,
Pubmed
Bortner,
A primary role for K+ and Na+ efflux in the activation of apoptosis.
1997,
Pubmed
Bortner,
Caspase independent/dependent regulation of K(+), cell shrinkage, and mitochondrial membrane potential during lymphocyte apoptosis.
1999,
Pubmed
Bruzzone,
Connections with connexins: the molecular basis of direct intercellular signaling.
1996,
Pubmed
Chen-Izu,
Opposing gates model for voltage gating of gap junction channels.
2001,
Pubmed
,
Xenbase
Dallaporta,
Potassium leakage during the apoptotic degradation phase.
1998,
Pubmed
Elenes,
Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43.
2001,
Pubmed
Estévez,
Nitric oxide-dependent production of cGMP supports the survival of rat embryonic motor neurons cultured with brain-derived neurotrophic factor.
1998,
Pubmed
Godwin,
In situ regulation of cell-cell communication by the cAMP-dependent protein kinase and protein kinase C.
1993,
Pubmed
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Gutkowska,
Hormonal regulation of natriuretic peptide system during induced ovarian follicular development in the rat.
1999,
Pubmed
Gómez-Angelats,
Cell volume regulation in immune cell apoptosis.
2000,
Pubmed
Harris,
Emerging issues of connexin channels: biophysics fills the gap.
2001,
Pubmed
Hirsch,
A novel cGMP-regulated K+ channel in immortalized human kidney epitheliall cells (IHKE-1).
1999,
Pubmed
Kanemitsu,
Cdc2-mediated phosphorylation of the gap junction protein, connexin43, during mitosis.
1998,
Pubmed
Keren-Tal,
Establishment of steroidogenic granulosa cell lines expressing follicle stimulating hormone receptors.
1993,
Pubmed
Krick,
Activation of K+ channels induces apoptosis in vascular smooth muscle cells.
2001,
Pubmed
Maeno,
Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis.
2000,
Pubmed
McGee,
Preantral ovarian follicles in serum-free culture: suppression of apoptosis after activation of the cyclic guanosine 3',5'-monophosphate pathway and stimulation of growth and differentiation by follicle-stimulating hormone.
1997,
Pubmed
Moreno,
Modulation of Kv3 potassium channels expressed in CHO cells by a nitric oxide-activated phosphatase.
2001,
Pubmed
Neyton,
Single-channel currents of an intercellular junction.
,
Pubmed
Ngezahayo,
Gap junctional permeability is affected by cell volume changes and modulates volume regulation.
1990,
Pubmed
Ngezahayo,
Inactivation of expressed and conducting rCx46 hemichannels by phosphorylation.
1998,
Pubmed
,
Xenbase
Ngezahayo,
Regulation of gap junctional coupling in isolated pancreatic acinar cell pairs by cholecystokinin-octapeptide, vasoactive intestinal peptide (VIP) and a VIP-antagonist.
1994,
Pubmed
Noubani,
B-type natriuretic peptide receptor expression and activity are hormonally regulated in rat ovarian cells.
2000,
Pubmed
Okuma,
Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary.
1996,
Pubmed
Perez,
Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells.
2000,
Pubmed
Schreiber,
Changes in gap junction connexin-43 messenger ribonucleic acid levels associated with rat ovarian follicular development as demonstrated by in situ hybridization.
1993,
Pubmed
Sommersberg,
Gap junction communication and connexin 43 gene expression in a rat granulosa cell line: regulation by follicle-stimulating hormone.
2000,
Pubmed
Somogyi,
Inhibition of electrical coupling in pairs of murine pancreatic acinar cells by OAG and isolated protein kinase C.
1989,
Pubmed
Sosinsky,
Mixing of connexins in gap junction membrane channels.
1995,
Pubmed
Steiner,
Functional characterization of canine connexin45.
1996,
Pubmed
,
Xenbase
Stergiopoulos,
Hetero-domain interactions as a mechanism for the regulation of connexin channels.
1999,
Pubmed
,
Xenbase
Takuma,
Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore.
2001,
Pubmed
Tilly,
Apoptosis and ovarian function.
1996,
Pubmed
Todt,
Hydrogen peroxide inhibits gap junctional coupling and modulates intracellular free calcium in cochlear Hensen cells.
2001,
Pubmed
Veenstra,
Selective dye and ionic permeability of gap junction channels formed by connexin45.
1994,
Pubmed
Veenstra,
Voltage clamp limitations of dual whole-cell gap junction current and voltage recordings. I. Conductance measurements.
2001,
Pubmed
White,
Genetic diseases and gene knockouts reveal diverse connexin functions.
1999,
Pubmed
White,
Multiple connexin proteins in single intercellular channels: connexin compatibility and functional consequences.
1996,
Pubmed
Wiesen,
Expression of connexin 43 gap junction messenger ribonucleic acid and protein during follicular atresia.
1994,
Pubmed
Willecke,
Structural and functional diversity of connexin genes in the mouse and human genome.
2002,
Pubmed
Wright,
Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.
2001,
Pubmed
Zhao,
Voltage gating of gap junctions in cochlear supporting cells: evidence for nonhomotypic channels.
2000,
Pubmed
Zhao,
Effect of membrane tension on gap junctional conductance of supporting cells in Corti's organ.
1998,
Pubmed
