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Plant Signal Behav
2013 Feb 01;82:e22813. doi: 10.4161/psb.22813.
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Allelic differences in Medicago truncatula NIP/LATD mutants correlate with their encoded proteins' transport activities in planta.
Salehin M
,
Huang YS
,
Bagchi R
,
Sherrier DJ
,
Dickstein R
.
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Medicago truncatula NIP/LATD gene, required for symbiotic nitrogen fixing nodule and root architecture development, encodes a member of the NRT1(PTR) family that demonstrates high-affinity nitrate transport in Xenopus laevis oocytes. Of three Mtnip/latd mutant proteins, one retains high-affinity nitrate transport in oocytes, while the other two are nitrate-transport defective. To further examine the mutant proteins' transport properties, the missense Mtnip/latd alleles were expressed in Arabidopsis thaliana chl1-5, resistant to the herbicide chlorate because of a deletion spanning the nitrate transporter AtNRT1.1(CHL1) gene. Mtnip-3 expression restored chlorate sensitivity in the Atchl1-5 mutant, similar to wild type MtNIP/LATD, while Mtnip-1 expression did not. The high-affinity nitrate transporter AtNRT2.1 gene was expressed in Mtnip-1 mutant roots; it did not complement, which could be caused by several factors. Together, these findings support the hypothesis that MtNIP/LATD may have another biochemical activity.
Figure 1.Mtnip-3, but not Mtnip-1, complements the chlorate-insensitivity phenotype of the Arabidopsis chl1–5 mutant. Arabidopsis control and test plants were treated with chlorate, a nitrate analog that can be converted to toxic chlorite after uptake.17,20 Two independent Atchl1–5/Mtnip-1 lines and three independent Atchl1–5/Mtnip-3 lines were tested; representative plants are shown. The genotype and transgene in each plant is indicated. Bar = 1 cm. The Mtnip-3 gene was able to confer chlorate sensitivity on Arabidopsis chl1–5 plants, similar to the MtNIP/LATD and AtNRT1.1 genes.
Figure 2. Fresh weight and chlorophyll content of chlorate treated Arabidopsis plants. The fresh weights and chlorophyll content of the Arabidopsis Col-0, Atchl1–5 and Atchl1–5 plants transformed with constructs and treated with chlorate, as in Figure 1, were measured. Asterisks mark values that are significantly different at the 1% level from the Atchl1–5 value, using the paired t-test. (A) The fresh weights of Atchl1–5 plants transformed with the Mtnip-1 gene were indistinguishable from Atchl1–5 plants, and the fresh weights of two of the Atchl1–5 lines transformed with Mtnip-3 were indistinguishable from those of Col-0 or Atchl1–5 transformed with either MtNIP/LATD and AtNRT1.1 genes. The third Atchl1–5/Mtnip-3 line has similar, but not identical, fresh weight to the other two. (B) The chlorophyll contents of Atchl1–5 plants transformed with the Mtnip-1 gene were similar to Atchl1–5 plants, and the chlorophyll contents of two of the Atchl1–5 lines transformed with Mtnip-3 were similar to those of Col-0 and to chlorophyll contents of Atchl1–5 transformed with either MtNIP/LATD and AtNRT1.1 genes. The third Atchl1–5/Mtnip-3 line had intermediate chlorophyll content.
Bagchi,
Functional assessment of the Medicago truncatula NIP/LATD protein demonstrates that it is a high-affinity nitrate transporter.
2012, Pubmed,
Xenbase
Bagchi,
Functional assessment of the Medicago truncatula NIP/LATD protein demonstrates that it is a high-affinity nitrate transporter.
2012,
Pubmed
,
Xenbase
Bright,
The LATD gene of Medicago truncatula is required for both nodule and root development.
2005,
Pubmed
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Harris,
Control of root architecture and nodulation by the LATD/NIP transporter.
2010,
Pubmed
Ho,
CHL1 functions as a nitrate sensor in plants.
2009,
Pubmed
Höfgen,
Storage of competent cells for Agrobacterium transformation.
1988,
Pubmed
Jeong,
A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.
2004,
Pubmed
,
Xenbase
Jones,
How rhizobial symbionts invade plants: the Sinorhizobium-Medicago model.
2007,
Pubmed
Kanno,
Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor.
2012,
Pubmed
Kotur,
Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1.
2012,
Pubmed
,
Xenbase
Kouchi,
How many peas in a pod? Legume genes responsible for mutualistic symbioses underground.
2010,
Pubmed
Krouk,
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants.
2010,
Pubmed
,
Xenbase
Laugier,
Regulation of high-affinity nitrate uptake in roots of Arabidopsis depends predominantly on posttranscriptional control of the NRT2.1/NAR2.1 transport system.
2012,
Pubmed
Liu,
Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation.
2003,
Pubmed
,
Xenbase
Liu,
CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake.
1999,
Pubmed
,
Xenbase
Morère-Le Paven,
Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula.
2011,
Pubmed
,
Xenbase
Muños,
Transcript profiling in the chl1-5 mutant of Arabidopsis reveals a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1.
2004,
Pubmed
Nour-Eldin,
NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds.
2012,
Pubmed
,
Xenbase
Oldroyd,
Coordinating nodule morphogenesis with rhizobial infection in legumes.
2008,
Pubmed
Sonti,
Arabidopsis mutants deficient in T-DNA integration.
1995,
Pubmed
Teillet,
api, A novel Medicago truncatula symbiotic mutant impaired in nodule primordium invasion.
2008,
Pubmed
Tsay,
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
1993,
Pubmed
,
Xenbase
Tsay,
Nitrate transporters and peptide transporters.
2007,
Pubmed
Veereshlingam,
nip, a symbiotic Medicago truncatula mutant that forms root nodules with aberrant infection threads and plant defense-like response.
2004,
Pubmed
Wilkinson,
Identification of the Arabidopsis CHL3 gene as the nitrate reductase structural gene NIA2.
1991,
Pubmed
Yendrek,
A putative transporter is essential for integrating nutrient and hormone signaling with lateral root growth and nodule development in Medicago truncatula.
2010,
Pubmed
