Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Siliques are Red1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques.
Ladwig F
,
Stahl M
,
Ludewig U
,
Hirner AA
,
Hammes UZ
,
Stadler R
,
Harter K
,
Koch W
.
???displayArticle.abstract???
Many membrane proteins are involved in the transport of nutrients in plants. While the import of amino acids into plant cells is, in principle, well understood, their export has been insufficiently described. Here, we present the identification and characterization of the membrane protein Siliques Are Red1 (SIAR1) from Arabidopsis (Arabidopsis thaliana) that is able to translocate amino acids bidirectionally into as well as out of the cell. Analyses in yeast and oocytes suggest a SIAR1-mediated export of amino acids. In Arabidopsis, SIAR1 localizes to the plasma membrane and is expressed in the vascular tissue, in the pericycle, in stamen, and in the chalazal seed coat of ovules and developing seeds. Mutant alleles of SIAR1 accumulate anthocyanins as a symptom of reduced amino acid content in the early stages of silique development. Our data demonstrate that the SIAR1-mediated export of amino acids plays an important role in organic nitrogen allocation and particularly in amino acid homeostasis in developing siliques.
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003, Pubmed
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003,
Pubmed
Bloor,
The structure of the major anthocyanin in Arabidopsis thaliana.
2002,
Pubmed
Bröer,
Amino acid transport across mammalian intestinal and renal epithelia.
2008,
Pubmed
Chen,
Sucrose efflux mediated by SWEET proteins as a key step for phloem transport.
2012,
Pubmed
Chillarón,
Heteromeric amino acid transporters: biochemistry, genetics, and physiology.
2001,
Pubmed
Dassler,
Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway.
2000,
Pubmed
Dettmer,
Essential role of the V-ATPase in male gametophyte development.
2005,
Pubmed
Diaz,
Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana.
2006,
Pubmed
Dündar,
BAT1, a bidirectional amino acid transporter in Arabidopsis.
2009,
Pubmed
Fischer,
Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids.
2002,
Pubmed
,
Xenbase
Fukasawa,
Identification and characterization of a Na(+)-independent neutral amino acid transporter that associates with the 4F2 heavy chain and exhibits substrate selectivity for small neutral D- and L-amino acids.
2000,
Pubmed
,
Xenbase
Gaymard,
Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap.
1998,
Pubmed
,
Xenbase
Hammes,
AtCAT6, a sink-tissue-localized transporter for essential amino acids in Arabidopsis.
2006,
Pubmed
,
Xenbase
Hirner,
Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll.
2006,
Pubmed
Hirner,
Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.
1998,
Pubmed
Imlau,
Cell-to-cell and long-distance trafficking of the green fluorescent protein in the phloem and symplastic unloading of the protein into sink tissues.
1999,
Pubmed
Jack,
The drug/metabolite transporter superfamily.
2001,
Pubmed
Kucsera,
Simple detection method for distinguishing dead and living yeast colonies.
2000,
Pubmed
Lalonde,
Transport mechanisms for organic forms of carbon and nitrogen between source and sink.
2004,
Pubmed
Le,
Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors.
2010,
Pubmed
Lee,
Selective expression of a novel high-affinity transport system for acidic and neutral amino acids in the tapetum cells of Arabidopsis flowers.
2004,
Pubmed
Lillo,
Nutrient depletion as a key factor for manipulating gene expression and product formation in different branches of the flavonoid pathway.
2008,
Pubmed
Lin,
Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport.
2008,
Pubmed
,
Xenbase
Lodwig,
Amino-acid cycling drives nitrogen fixation in the legume-Rhizobium symbiosis.
2003,
Pubmed
Meier,
Activation of system L heterodimeric amino acid exchangers by intracellular substrates.
2002,
Pubmed
,
Xenbase
Miller,
Xenopus oocytes as an expression system for plant transporters.
2000,
Pubmed
,
Xenbase
Negrutiu,
Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.
1987,
Pubmed
Okumoto,
High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis.
2002,
Pubmed
Okumoto,
Amino acid export in plants: a missing link in nitrogen cycling.
2011,
Pubmed
Pilot,
Overexpression of GLUTAMINE DUMPER1 leads to hypersecretion of glutamine from Hydathodes of Arabidopsis leaves.
2004,
Pubmed
Pratelli,
Stimulation of nonselective amino acid export by glutamine dumper proteins.
2010,
Pubmed
Ranocha,
Walls are thin 1 (WAT1), an Arabidopsis homolog of Medicago truncatula NODULIN21, is a tonoplast-localized protein required for secondary wall formation in fibers.
2010,
Pubmed
Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed
Rentsch,
Transporters for uptake and allocation of organic nitrogen compounds in plants.
2007,
Pubmed
Riesmeier,
Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning.
1994,
Pubmed
Rodríguez-Ezpeleta,
Monophyly of primary photosynthetic eukaryotes: green plants, red algae, and glaucophytes.
2005,
Pubmed
Sanders,
AAP1 regulates import of amino acids into developing Arabidopsis embryos.
2009,
Pubmed
Schmid,
A gene expression map of Arabidopsis thaliana development.
2005,
Pubmed
Schmidt,
The amino acid permease AAP8 is important for early seed development in Arabidopsis thaliana.
2007,
Pubmed
Schwacke,
ARAMEMNON, a novel database for Arabidopsis integral membrane proteins.
2003,
Pubmed
Schwacke,
LeProT1, a transporter for proline, glycine betaine, and gamma-amino butyric acid in tomato pollen.
1999,
Pubmed
Soussi-Boudekou,
Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae.
1997,
Pubmed
Stadler,
Cell-to-cell movement of green fluorescent protein reveals post-phloem transport in the outer integument and identifies symplastic domains in Arabidopsis seeds and embryos.
2005,
Pubmed
Takano,
Arabidopsis boron transporter for xylem loading.
2002,
Pubmed
Tanaka,
The histidine permease gene (HIP1) of Saccharomyces cerevisiae.
1985,
Pubmed
Tohge,
Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
2005,
Pubmed
Verbruggen,
Osmoregulation of a pyrroline-5-carboxylate reductase gene in Arabidopsis thaliana.
1993,
Pubmed
Verbruggen,
Proline accumulation in plants: a review.
2008,
Pubmed
Werner,
A dual switch in phloem unloading during ovule development in Arabidopsis.
2011,
Pubmed
Yang,
H-independent glutamine transport in plant root tips.
2010,
Pubmed