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J Physiol
2003 Nov 15;553Pt 1:13-9. doi: 10.1113/jphysiol.2003.054551.
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Small conductance Ca2+-activated K+ channels formed by the expression of rat SK1 and SK2 genes in HEK 293 cells.
Benton DC
,
Monaghan AS
,
Hosseini R
,
Bahia PK
,
Haylett DG
,
Moss GW
.
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The rat SK1 gene (rSK1) does not form functional Ca2+-activated potassium channels when expressed alone in mammalian cell lines. Using a selective antibody to the rSK1 subunit and a yellow fluorescent protein (YFP) tag we have discovered that rSK1 expression produces protein that remains largely at intracellular locations. We tested the idea that rSK1 may need an expression partner, rSK2, in order to form functional channels. When rSK1 was co-expressed with rSK2 in HEK 293 cells it increased the current magnitude by 77 +/- 34% (as compared with cells expressing rSK2 alone). Co-expression of rSK1 with rSK2 also changed the channel pharmacology. The sensitivity of SK current to block by apamin was reduced approximately 16-fold from an IC50 of 94 pM (for SK2 alone) to 1.4 nM (for SK2 and SK1 together). The sensitivity to block by UCL 1848 (a potent small molecule blocker of SK channels) was similarly reduced, approximately 26-fold, from an IC50 of 110 pM to 2.9 nM. These data clearly demonstrate that rSK1 and rSK2 subunits interact. The most likely explanation for this is that the subunits are able to form heteromeric assemblies.
Benton,
Small conductance Ca2+-activated K+ channels formed by the expression of rat SK1 and SK2 genes in HEK 293 cells.
2003, Pubmed,
Xenbase
Benton,
Small conductance Ca2+-activated K+ channels formed by the expression of rat SK1 and SK2 genes in HEK 293 cells.
2003,
Pubmed
,
Xenbase
Bowden,
Somatic colocalization of rat SK1 and D class (Ca(v)1.2) L-type calcium channels in rat CA1 hippocampal pyramidal neurons.
2001,
Pubmed
Chandy,
Isolation of a novel potassium channel gene hSKCa3 containing a polymorphic CAG repeat: a candidate for schizophrenia and bipolar disorder?
1998,
Pubmed
Chen,
bis-Quinolinium cyclophanes: 8,14-diaza-1,7(1, 4)-diquinolinacyclotetradecaphane (UCL 1848), a highly potent and selective, nonpeptidic blocker of the apamin-sensitive Ca(2+)-activated K(+) channel.
2000,
Pubmed
Faber,
Physiological role of calcium-activated potassium currents in the rat lateral amygdala.
2002,
Pubmed
Grunnet,
Apamin interacts with all subtypes of cloned small-conductance Ca2+-activated K+ channels.
2001,
Pubmed
,
Xenbase
Hosseini,
SK3 is an important component of K(+) channels mediating the afterhyperpolarization in cultured rat SCG neurones.
2001,
Pubmed
Ishii,
Determinants of apamin and d-tubocurarine block in SK potassium channels.
1997,
Pubmed
,
Xenbase
Joiner,
hSK4, a member of a novel subfamily of calcium-activated potassium channels.
1997,
Pubmed
Krapivinsky,
The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins.
1995,
Pubmed
,
Xenbase
Köhler,
Small-conductance, calcium-activated potassium channels from mammalian brain.
1996,
Pubmed
,
Xenbase
Ottschytsch,
Obligatory heterotetramerization of three previously uncharacterized Kv channel alpha-subunits identified in the human genome.
2002,
Pubmed
Pedarzani,
Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons.
2002,
Pubmed
Post,
Kv2.1 and electrically silent Kv6.1 potassium channel subunits combine and express a novel current.
1996,
Pubmed
,
Xenbase
Sah,
Channels underlying neuronal calcium-activated potassium currents.
2002,
Pubmed
Sailer,
Regional differences in distribution and functional expression of small-conductance Ca2+-activated K+ channels in rat brain.
2002,
Pubmed
,
Xenbase
Shah,
The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines.
2000,
Pubmed
,
Xenbase
Stocker,
An apamin-sensitive Ca2+-activated K+ current in hippocampal pyramidal neurons.
1999,
Pubmed
Stocker,
Differential distribution of three Ca(2+)-activated K(+) channel subunits, SK1, SK2, and SK3, in the adult rat central nervous system.
2000,
Pubmed
Strøbaek,
Pharmacological characterization of small-conductance Ca(2+)-activated K(+) channels stably expressed in HEK 293 cells.
2000,
Pubmed