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J Biol Chem
2012 Jun 15;28725:21130-41. doi: 10.1074/jbc.M112.347807.
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Intracellular trafficking of FXYD1 (phospholemman) and FXYD7 proteins in Xenopus oocytes and mammalian cells.
Moshitzky S
,
Asher C
,
Garty H
.
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FXYD proteins are a group of short single-span transmembrane proteins that interact with the Na(+)/K(+) ATPase and modulate its kinetic properties. This study characterizes intracellular trafficking of two FXYD family members, FXYD1 (phospholemman (PLM)) and FXYD7. Surface expression of PLM in Xenopus oocytes requires coexpression with the Na(+)/K(+) ATPase. On the other hand, the Na(+)/Ca(2+) exchanger, another PLM-interacting protein could not drive it to the cell surface. The Na(+)/K(+) ATPase-dependent surface expression of PLM could be facilitated by either a phosphorylation-mimicking mutation at Thr-69 or a truncation of three terminal arginine residues. Unlike PLM, FXYD7 could translocate to the cell surface of Xenopus oocytes independently of the coexpression of α1β1 Na(+)/K(+) ATPase. The Na(+)/K(+) ATPase-independent membrane translocation of FXYD7 requires O-glycosylation of at least two of three conserved threonines in its ectodomain. Subsequent experiments in mammalian cells confirmed the role of conserved extracellular threonine residues and demonstrated that FXYD7 protein, in which these have been mutated to alanine, is trapped in the endoplasmic reticulum and Golgi apparatus.
Ahlers,
Identification of an endogenous inhibitor of the cardiac Na+/Ca2+ exchanger, phospholemman.
2005, Pubmed
Ahlers,
Identification of an endogenous inhibitor of the cardiac Na+/Ca2+ exchanger, phospholemman.
2005,
Pubmed
Attali,
A corticosteroid-induced gene expressing an "IsK-like" K+ channel activity in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Bibert,
Phosphorylation of phospholemman (FXYD1) by protein kinases A and C modulates distinct Na,K-ATPase isozymes.
2008,
Pubmed
,
Xenbase
Bossuyt,
Phospholemman phosphorylation alters its fluorescence resonance energy transfer with the Na/K-ATPase pump.
2006,
Pubmed
Béguin,
FXYD7 is a brain-specific regulator of Na,K-ATPase alpha 1-beta isozymes.
2002,
Pubmed
,
Xenbase
Chen,
Characterization of the human and rat phospholemman (PLM) cDNAs and localization of the human PLM gene to chromosome 19q13.1.
1997,
Pubmed
,
Xenbase
Cheung,
Regulation of cardiac contractility: high time for FXYD.
2008,
Pubmed
Crambert,
FXYD7, the first brain- and isoform-specific regulator of Na,K-ATPase: biosynthesis and function of its posttranslational modifications.
2003,
Pubmed
Crambert,
Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties.
2002,
Pubmed
,
Xenbase
Crambert,
FXYD7, mapping of functional sites involved in endoplasmic reticulum export, association with and regulation of Na,K-ATPase.
2004,
Pubmed
,
Xenbase
Despa,
Phospholemman-phosphorylation mediates the beta-adrenergic effects on Na/K pump function in cardiac myocytes.
2005,
Pubmed
Fuller,
FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C.
2009,
Pubmed
Füzesi,
Covalent cross-links between the gamma subunit (FXYD2) and alpha and beta subunits of Na,K-ATPase: modeling the alpha-gamma interaction.
2005,
Pubmed
Garty,
Role of FXYD proteins in ion transport.
2006,
Pubmed
Geering,
Oligomerization and maturation of Na,K-ATPase: functional interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit.
1996,
Pubmed
,
Xenbase
Geering,
FXYD proteins: new regulators of Na-K-ATPase.
2006,
Pubmed
Guo,
Amino acid substitutions in the FXYD motif enhance phospholemman-induced modulation of cardiac L-type calcium channels.
2010,
Pubmed
Han,
Phospholemman phosphorylation mediates the protein kinase C-dependent effects on Na+/K+ pump function in cardiac myocytes.
2006,
Pubmed
Hanisch,
O-glycosylation of the mucin type.
2001,
Pubmed
Hennebicq-Reig,
Permanent exposure of mucin-secreting HT-29 cells to benzyl-N-acetyl-alpha-D-galactosaminide induces abnormal O-glycosylation of mucins and inhibits constitutive and stimulated MUC5AC secretion.
1998,
Pubmed
Ino,
Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis.
2002,
Pubmed
Kanno,
Increased plasma membrane localization of O-glycosylation-deficient mutant of synaptotagmin I in PC12 cells.
2008,
Pubmed
Kucik,
Weak dependence of mobility of membrane protein aggregates on aggregate size supports a viscous model of retardation of diffusion.
1999,
Pubmed
Lansbery,
Cytoplasmic targeting signals mediate delivery of phospholemman to the plasma membrane.
2006,
Pubmed
Lee,
Bi-directional protein transport between the ER and Golgi.
2004,
Pubmed
Li,
Role of the transmembrane domain of FXYD7 in structural and functional interactions with Na,K-ATPase.
2005,
Pubmed
,
Xenbase
Lifshitz,
Purification of the human alpha2 Isoform of Na,K-ATPase expressed in Pichia pastoris. Stabilization by lipids and FXYD1.
2007,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lubarski,
Structural and functional interactions between FXYD5 and the Na+-K+-ATPase.
2007,
Pubmed
,
Xenbase
Lubarski,
FXYD5 (dysadherin) regulates the paracellular permeability in cultured kidney collecting duct cells.
2011,
Pubmed
Madhani,
Phospholemman Ser69 phosphorylation contributes to sildenafil-induced cardioprotection against reperfusion injury.
2010,
Pubmed
Maryon,
O-linked glycosylation at threonine 27 protects the copper transporter hCTR1 from proteolytic cleavage in mammalian cells.
2007,
Pubmed
Miller,
FXYD5 modulates Na+ absorption and is increased in cystic fibrosis airway epithelia.
2008,
Pubmed
Minor,
The gamma subunit of the Na,K-ATPase induces cation channel activity.
1998,
Pubmed
,
Xenbase
Mishra,
FXYD proteins stabilize Na,K-ATPase: amplification of specific phosphatidylserine-protein interactions.
2011,
Pubmed
Mohler,
Ankyrin-B coordinates the Na/K ATPase, Na/Ca exchanger, and InsP3 receptor in a cardiac T-tubule/SR microdomain.
2005,
Pubmed
Moorman,
Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Morrison,
Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Mounsey,
Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases.
1999,
Pubmed
,
Xenbase
Nam,
Dysadherin: a new player in cancer progression.
2007,
Pubmed
Palmer,
Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium.
1991,
Pubmed
Potter,
Role of N- and O-glycans in polarized biosynthetic sorting.
2006,
Pubmed
Shinoda,
Crystal structure of the sodium-potassium pump at 2.4 A resolution.
2009,
Pubmed
Sigal,
Generation of a fluorescently labeled endogenous protein library in living human cells.
2007,
Pubmed
Silverman,
Serine 68 phosphorylation of phospholemman: acute isoform-specific activation of cardiac Na/K ATPase.
2005,
Pubmed
Song,
Serine 68 of phospholemman is critical in modulation of contractility, [Ca2+]i transients, and Na+/Ca2+ exchange in adult rat cardiac myocytes.
2005,
Pubmed
Song,
Constitutive overexpression of phosphomimetic phospholemman S68E mutant results in arrhythmias, early mortality, and heart failure: potential involvement of Na+/Ca2+ exchanger.
2012,
Pubmed
Stoos,
Characterization of a mouse cortical collecting duct cell line.
1991,
Pubmed
Sweadner,
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression.
2000,
Pubmed
Wang,
Cytoplasmic tail of phospholemman interacts with the intracellular loop of the cardiac Na+/Ca2+ exchanger.
2006,
Pubmed
Wang,
Regulation of in vivo cardiac contractility by phospholemman: role of Na+/Ca2+ exchange.
2011,
Pubmed
Wilchek,
p-Diazobenzoyl biocytin--a new biotinylating reagent for the labeling of tyrosines and histidines in proteins.
1986,
Pubmed
Zhang,
Phospholemman inhibition of the cardiac Na+/Ca2+ exchanger. Role of phosphorylation.
2006,
Pubmed
Zhang,
Phospholemman modulates Na+/Ca2+ exchange in adult rat cardiac myocytes.
2003,
Pubmed
Zhang,
Phospholemman regulates cardiac Na+/Ca2+ exchanger by interacting with the exchanger's proximal linker domain.
2009,
Pubmed
Zhu,
Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis.
2001,
Pubmed
