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Cytotechnology
1998 Mar 01;262:153-64. doi: 10.1023/A:1007934216507.
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Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme.
Furukawa K
,
Ohsuye K
.
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In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3µ-1S), producing a C-terminal α-amidating enzyme (799BglIIα-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 °C) led to the following phenomena: [1] inhibited cell growth, [2] enhanced cellular productivity of the recombinant protein, [3] maintained high cell viability, [4] suppressed medium consumption, and [5] suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglIIα-AE.
Bloemkolk,
Effect of temperature on hybridoma cell cycle and MAb production.
1992, Pubmed
Bloemkolk,
Effect of temperature on hybridoma cell cycle and MAb production.
1992,
Pubmed
Borth,
Growth and production kinetics of human x mouse and mouse hybridoma cells at reduced temperature and serum content.
1992,
Pubmed
Borys,
Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by chinese hamster ovary cells in a pH-dependent manner.
1994,
Pubmed
Borys,
Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese hamster ovary (CHO) cells.
1993,
Pubmed
Bradbury,
Mechanism of C-terminal amide formation by pituitary enzymes.
1982,
Pubmed
Eipper,
Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.
1983,
Pubmed
Giard,
Examination of parameters affecting human interferon production with microcarrier-grown fibroblast cells.
1980,
Pubmed
Giard,
Effect of temperature on the production of human fibroblast interferon.
1982,
Pubmed
Goldblum,
Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress.
1990,
Pubmed
Goochee,
Environmental effects on protein glycosylation.
1990,
Pubmed
Hahn,
Growth-associated glycosylation of transferrin secreted by HepG2 cells.
1992,
Pubmed
Hayter,
Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human interferon-gamma.
1992,
Pubmed
Hosoi,
Optimization of cell culture conditions for G-CSF (granulocyte-colony stimulating factor) production by genetically engineered Namalwa KJM-1 cells.
1991,
Pubmed
Kato,
Two enzymes concerned in peptide hormone alpha-amidation are synthesized from a single mRNA.
1990,
Pubmed
Kojima,
Enhanced production of interferon by temperature shift-down from 37 C to 25 C in rabbit cell cultures stimulated with Newcastle disease virus.
1974,
Pubmed
Lin,
Production of tPA in recombinant CHO cells under oxygen-limited conditions.
1993,
Pubmed
McQueen,
Flow effects on the viability and lysis of suspended mammalian cells.
1987,
Pubmed
Merten,
Batch production and growth kinetics of hybridomas.
1988,
Pubmed
Mizuno,
Cloning and sequence of cDNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis.
1987,
Pubmed
,
Xenbase
Mizuno,
Peptide C-terminal alpha-amidating enzyme purified to homogeneity from Xenopus laevis skin.
1986,
Pubmed
,
Xenbase
Mochizuki,
Enhanced production of human monoclonal antibodies by the use of fructose in serum-free hybridoma culture media.
1993,
Pubmed
Ohsuye,
Cloning of cDNA encoding a new peptide C-terminal alpha-amidating enzyme having a putative membrane-spanning domain from Xenopus laevis skin.
1988,
Pubmed
,
Xenbase
Ozturk,
Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor.
1991,
Pubmed
Patel,
Different culture methods lead to differences in glycosylation of a murine IgG monoclonal antibody.
1992,
Pubmed
Reuveny,
Factors affecting cell growth and monoclonal antibody production in stirred reactors.
1986,
Pubmed
Sureshkumar,
The influence of temperature on a mouse-mouse hybridoma growth and monoclonal antibody production.
1991,
Pubmed
Tachibana,
Modified antigen-binding of human antibodies with glycosylation variations of the light chains produced in sugar-limited human hybridoma cultures.
1996,
Pubmed
Takagi,
Comparison of the optimal culture conditions for cell growth and tissue plasminogen activator production by human embryo lung cells on microcarriers.
1994,
Pubmed
Vilcek,
Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature.
1973,
Pubmed
Weidemann,
Low temperature cultivation--a step towards process optimisation.
1994,
Pubmed