Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2011 Dec 09;28649:42414-42425. doi: 10.1074/jbc.M111.271064.
Show Gene links
Show Anatomy links
Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.
Zhang Y
,
Yang X
,
Gui B
,
Xie G
,
Zhang D
,
Shang Y
,
Liang J
.
???displayArticle.abstract???
Polycomb group proteins play essential roles in transcriptional regulation of multiple gene families involved in various pathophysiological processes. It is believed that Polycomb Repressive Complex 2 (PRC2) is targeted to chromatin by the EED subunit to methylate histone H3 lysine 27 (H3K27), leading to a repressive chromatin state that inhibits gene expression. Here we report that the chromodomain-containing protein CDYL specifically recognizes di- and tri-methylated H3K27 (H3K27me2 and H3K27me3) and directly interacts with EZH2, the catalytic subunit of PRC2. We show that CDYL dramatically enhances the methyltransferase activity of PRC2 toward oligonucleosome substrates in vitro. Genome-wide analysis of CDYL targets by ChIP sequencing revealed that CDYL and PRC2 share a number of genomic targets. CDYL is required for chromatin targeting and maximal enzymatic activity of PRC2 at their common target sites. Our experiments indicate that CDYL functions as a molecular bridge between PRC2 and the repressive chromatin mark H3K27me3, forming a positive feedback loop to facilitate the establishment and propagation of H3K27me3 modifications along the chromatin.
Bailey,
Fitting a mixture model by expectation maximization to discover motifs in biopolymers.
1994, Pubmed
Bailey,
Fitting a mixture model by expectation maximization to discover motifs in biopolymers.
1994,
Pubmed
Berger,
The complex language of chromatin regulation during transcription.
2007,
Pubmed
Bracken,
Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
2006,
Pubmed
Campos,
Histones: annotating chromatin.
2009,
Pubmed
Cao,
Role of histone H3 lysine 27 methylation in Polycomb-group silencing.
2002,
Pubmed
Cao,
Role of hPHF1 in H3K27 methylation and Hox gene silencing.
2008,
Pubmed
Caron,
Cdyl: a new transcriptional co-repressor.
2003,
Pubmed
Dodd,
Theoretical analysis of epigenetic cell memory by nucleosome modification.
2007,
Pubmed
Dorus,
The CDY-related gene family: coordinated evolution in copy number, expression profile and protein sequence.
2003,
Pubmed
Franz,
Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin association.
2009,
Pubmed
Goldberg,
Epigenetics: a landscape takes shape.
2007,
Pubmed
Iwase,
The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases.
2007,
Pubmed
Ji,
CEAS: cis-regulatory element annotation system.
2006,
Pubmed
Joseph,
Integrative model of genomic factors for determining binding site selection by estrogen receptor-α.
2010,
Pubmed
Kaufman,
Chromatin as a potential carrier of heritable information.
2010,
Pubmed
Kim,
Tudor, MBT and chromo domains gauge the degree of lysine methylation.
2006,
Pubmed
Kleer,
EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells.
2003,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Ku,
Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains.
2008,
Pubmed
Kuppuswamy,
Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex.
2008,
Pubmed
Kuzmichev,
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein.
2002,
Pubmed
Lee,
Control of developmental regulators by Polycomb in human embryonic stem cells.
2006,
Pubmed
Li,
Functional consequences of new exon acquisition in mammalian chromodomain Y-like (CDYL) genes.
2007,
Pubmed
Li,
ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis.
2009,
Pubmed
Loyola,
Histone deposition and chromatin assembly by RSF.
2003,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Margueron,
Role of the polycomb protein EED in the propagation of repressive histone marks.
2009,
Pubmed
Margueron,
The Polycomb complex PRC2 and its mark in life.
2011,
Pubmed
Moazed,
Mechanisms for the inheritance of chromatin states.
2011,
Pubmed
Mori,
A common silencer element in the SCG10 and type II Na+ channel genes binds a factor present in nonneuronal cells but not in neuronal cells.
1992,
Pubmed
Mortazavi,
Comparative genomics modeling of the NRSF/REST repressor network: from single conserved sites to genome-wide repertoire.
2006,
Pubmed
Mulligan,
CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation.
2008,
Pubmed
Müller,
Histone methyltransferase activity of a Drosophila Polycomb group repressor complex.
2002,
Pubmed
Sarma,
Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo.
2008,
Pubmed
Schoeftner,
Recruitment of PRC1 function at the initiation of X inactivation independent of PRC2 and silencing.
2006,
Pubmed
Shang,
Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.
2000,
Pubmed
Shi,
Coordinated histone modifications mediated by a CtBP co-repressor complex.
2003,
Pubmed
Shi,
Histone demethylase JMJD2B coordinates H3K4/H3K9 methylation and promotes hormonally responsive breast carcinogenesis.
2011,
Pubmed
Shi,
ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression.
2006,
Pubmed
Shi,
Integration of estrogen and Wnt signaling circuits by the polycomb group protein EZH2 in breast cancer cells.
2007,
Pubmed
Sims,
Methods to identify and functionally analyze factors that specifically recognize histone lysine methylation.
2006,
Pubmed
,
Xenbase
Sing,
A vertebrate Polycomb response element governs segmentation of the posterior hindbrain.
2009,
Pubmed
Tan,
Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.
2007,
Pubmed
Taverna,
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
2007,
Pubmed
Tolhuis,
Genome-wide profiling of PRC1 and PRC2 Polycomb chromatin binding in Drosophila melanogaster.
2006,
Pubmed
Vermeulen,
Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.
2010,
Pubmed
Wang,
Role of histone H2A ubiquitination in Polycomb silencing.
2004,
Pubmed
Wang,
LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer.
2009,
Pubmed
Wu,
Hypomethylation-linked activation of PAX2 mediates tamoxifen-stimulated endometrial carcinogenesis.
2005,
Pubmed
Xu,
Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2).
2010,
Pubmed
Yin,
Molecular mechanisms involved in the growth stimulation of breast cancer cells by leptin.
2004,
Pubmed
Zhang,
Differential gene regulation by the SRC family of coactivators.
2004,
Pubmed
Zhang,
Mechanism of Polycomb group gene silencing.
2004,
Pubmed
Zhang,
Model-based analysis of ChIP-Seq (MACS).
2008,
Pubmed