XB-ART-4485
Biosci Biotechnol Biochem
2003 Oct 01;6710:2291-3.
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Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na+ transport.
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Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.
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