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J Biol Chem
2011 Nov 04;28644:38397-38407. doi: 10.1074/jbc.M111.221713.
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Homeostasis of extracellular ATP in human erythrocytes.
Montalbetti N, Leal Denis MF, Pignataro OP, Kobatake E, Lazarowski ER, Schwarzbaum PJ.
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We explored the intra- and extracellular processes governing the kinetics of extracellular ATP (ATPe) in human erythrocytes stimulated with agents that increase cAMP. Using the luciferin-luciferase reaction in off-line luminometry we found both direct adenylyl cyclase activation by forskolin and indirect activation through β-adrenergic stimulation with isoproterenol-enhanced [ATP]e in a concentration-dependent manner. A mixture (3V) containing a combination of these agents and the phosphodiesterase inhibitor papaverine activated ATP release, leading to a 3-fold increase in [ATP]e, and caused increases in cAMP concentration (3-fold for forskolin + papaverine, and 10-fold for 3V). The pannexin 1 inhibitor carbenoxolone and a pannexin 1 blocking peptide ((10)Panx1) decreased [ATP]e by 75-84%. The residual efflux of ATP resulted from unavoidable mechanical perturbations stimulating a novel, carbenoxolone-insensitive pathway. In real-time luminometry experiments using soluble luciferase, addition of 3V led to an acute increase in [ATP]e to a constant value of ∼1 pmol × (10(6) cells)(-1). A similar treatment using a surface attached luciferase (proA-luc) triggered a rapid accumulation of surface ATP levels to a peak concentration of 2.4 pmol × (10(6) cells)(-1), followed by a slower exponential decay (t(½) = 3.7 min) to a constant value of 1.3 pmol × (10(6) cells)(-1). Both for soluble luciferase and proA-luc, ATP efflux was fully blocked by carbenoxolone, pointing to a 3V-induced mechanism of ATP release mediated by pannexin 1. Ecto-ATPase activity was extremely low (∼28 fmol × (10(6) cells min)(-1)), but nevertheless physiologically relevant considering the high density of erythrocytes in human blood.
Bao,
Pannexin membrane channels are mechanosensitive conduits for ATP.
2004, Pubmed,
Xenbase
Bao,
Pannexin membrane channels are mechanosensitive conduits for ATP.
2004,
Pubmed
,
Xenbase Beigi,
Detection of local ATP release from activated platelets using cell surface-attached firefly luciferase.
1999,
Pubmed Bencic,
Ecto-ATPase activity of vertebrate blood cells.
1997,
Pubmed Burnstock,
Dual control of vascular tone and remodelling by ATP released from nerves and endothelial cells.
2008,
Pubmed Chara,
Kinetics of extracellular ATP from goldfish hepatocytes: a lesson from mathematical modeling.
2009,
Pubmed Coade,
Metabolism of adenine nucleotides in human blood.
1989,
Pubmed Del Punta,
Nitric oxide inhibits Leydig cell steroidogenesis.
1996,
Pubmed Ellsworth,
Erythrocytes: oxygen sensors and modulators of vascular tone.
2009,
Pubmed Erlinge,
P2 receptors in cardiovascular regulation and disease.
2008,
Pubmed EYLAR,
The contribution of sialic acid to the surface charge of the erythrocyte.
1962,
Pubmed Gheorghiu,
Modeling of basolateral ATP release induced by hypotonic treatment in A6 cells.
2004,
Pubmed
,
Xenbase Gorman,
Measurement of adenine nucleotides in plasma.
2003,
Pubmed Gorman,
Human plasma ATP concentration.
2007,
Pubmed Hanson,
Insulin inhibits low oxygen-induced ATP release from human erythrocytes: implication for vascular control.
2009,
Pubmed Hategan,
Topographical pattern dynamics in passive adhesion of cell membranes.
2004,
Pubmed Jensen,
The dual roles of red blood cells in tissue oxygen delivery: oxygen carriers and regulators of local blood flow.
2009,
Pubmed Joseph,
Colocalization of ATP release sites and ecto-ATPase activity at the extracellular surface of human astrocytes.
2003,
Pubmed Koziak,
Analysis of CD39/ATP diphosphohydrolase (ATPDase) expression in endothelial cells, platelets and leukocytes.
1999,
Pubmed Kunapuli,
P2 receptor subtypes in the cardiovascular system.
1998,
Pubmed Kwant,
The erythrocyte ghost is a perfect osmometer.
1970,
Pubmed Lazarowski,
Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules.
2003,
Pubmed Lecklin,
The adrenergic volume changes of immature and mature rainbow trout (Oncorhynchus mykiss) erythrocytes.
2000,
Pubmed Locovei,
Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex.
2007,
Pubmed
,
Xenbase Locovei,
Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium.
2006,
Pubmed
,
Xenbase Locovei,
Pannexin 1 in erythrocytes: function without a gap.
2006,
Pubmed
,
Xenbase LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Lüthje,
Catabolism of Ap4A and Ap3A in whole blood. The dinucleotides are long-lived signal molecules in the blood ending up as intracellular ATP in the erythrocytes.
1988,
Pubmed Misiti,
Amyloid peptide inhibits ATP release from human erythrocytes.
2008,
Pubmed Mondillo,
Dual role of histamine in modulation of Leydig cell steroidogenesis via HRH1 and HRH2 receptor subtypes.
2005,
Pubmed Mondillo,
Involvement of nitric oxide synthase in the mechanism of histamine-induced inhibition of Leydig cell steroidogenesis via histamine receptor subtypes in Sprague-Dawley rats.
2009,
Pubmed Olearczyk,
Nitric oxide inhibits ATP release from erythrocytes.
2004,
Pubmed Olearczyk,
NO inhibits signal transduction pathway for ATP release from erythrocytes via its action on heterotrimeric G protein Gi.
2004,
Pubmed Olearczyk,
Heterotrimeric G protein Gi is involved in a signal transduction pathway for ATP release from erythrocytes.
2004,
Pubmed Olearczyk,
Receptor-mediated activation of the heterotrimeric G-protein Gs results in ATP release from erythrocytes.
2001,
Pubmed Pafundo,
Kinetics of ATP release and cell volume regulation of hyposmotically challenged goldfish hepatocytes.
2008,
Pubmed Pafundo,
A volume regulatory response can be triggered by nucleosides in human erythrocytes, a perfect osmometer no longer.
2010,
Pubmed Pelegrin,
Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor.
2006,
Pubmed Pirotton,
Endothelial P2-purinoceptors: subtypes and signal transduction.
1996,
Pubmed Qiu,
A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP.
2009,
Pubmed
,
Xenbase Remaley,
Expression of human glycophorin A in wild type and glycosylation-deficient Chinese hamster ovary cells. Role of N- and O-linked glycosylation in cell surface expression.
1991,
Pubmed Robson,
The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance.
2006,
Pubmed Schwarzbaum,
Functional role of ecto-ATPase activity in goldfish hepatocytes.
1998,
Pubmed Schwarzbaum,
An unexpected effect of ATP on the ratio between activity and phosphoenzyme level of Na+/K(+)-ATPase in steady state.
1995,
Pubmed Schwiebert,
Extracellular ATP as a signaling molecule for epithelial cells.
2003,
Pubmed Sévigny,
Purification of the blood vessel ATP diphosphohydrolase, identification and localisation by immunological techniques.
1997,
Pubmed Sprague,
Red not dead: signaling in and from erythrocytes.
2007,
Pubmed Sprague,
Prostacyclin analogs stimulate receptor-mediated cAMP synthesis and ATP release from rabbit and human erythrocytes.
2008,
Pubmed Sprague,
Participation of cAMP in a signal-transduction pathway relating erythrocyte deformation to ATP release.
2001,
Pubmed Sprague,
Deformation-induced ATP release from red blood cells requires CFTR activity.
1998,
Pubmed Sridharan,
Pannexin 1 is the conduit for low oxygen tension-induced ATP release from human erythrocytes.
2010,
Pubmed Steiner,
Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate.
1969,
Pubmed Strehler,
Bioluminescence assay: principles and practice.
1968,
Pubmed Vázquez,
Determination of hemoglobin through its peroxidase activity on chlorpromazine.
1991,
Pubmed Wang,
ADP acting on P2Y13 receptors is a negative feedback pathway for ATP release from human red blood cells.
2005,
Pubmed Wang,
Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters.
2007,
Pubmed
,
Xenbase Zhang,
Genetically fused protein A-luciferase for immunological blotting analyses.
2000,
Pubmed
