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Nishibori Y
,
Katayama K
,
Parikka M
,
Oddsson A
,
Nukui M
,
Hultenby K
,
Wernerson A
,
He B
,
Ebarasi L
,
Raschperger E
,
Norlin J
,
Uhlén M
,
Patrakka J
,
Betsholtz C
,
Tryggvason K
.
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Unbiased transcriptome profiling and functional genomics approaches identified glucocorticoid-induced transcript 1 (GLCCI1) as being a transcript highly specific for the glomerulus, but its role in glomerular development and disease is unknown. Here, we report that mouse glomeruli express far greater amounts of Glcci1 protein compared with the rest of the kidney. RT-PCR and Western blotting demonstrated that mouse glomerular Glcci1 is approximately 60 kD and localizes to the cytoplasm of podocytes in mature glomeruli. In the fetal kidney, intense Glcci1 expression occurs at the capillary-loop stage of glomerular development. Using gene knockdown in zebrafish with morpholinos, morphants lacking Glcci1 function had collapsed glomeruli with foot-process effacement. Permeability studies of the glomerular filtration barrier in these zebrafish morphants demonstrated a disruption of the selective glomerular permeability filter. Taken together, these data suggest that Glcci1 promotes the normal development and maintenance of podocyte structure and function.
Agaton,
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
2003, Pubmed
Agaton,
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
2003,
Pubmed
Chapman,
Isolation of differentially expressed sequence tags from steroid-responsive cells using mRNA differential display.
1995,
Pubmed
Chapman,
Transcriptional control of steroid-regulated apoptosis in murine thymoma cells.
1996,
Pubmed
Drummond,
Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function.
1998,
Pubmed
Ebarasi,
A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier.
2009,
Pubmed
,
Xenbase
FARQUHAR,
Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall.
1961,
Pubmed
Galloway,
Combinatorial regulation of novel erythroid gene expression in zebrafish.
2008,
Pubmed
He,
Podocin-green fluorescence protein allows visualization and functional analysis of podocytes.
2011,
Pubmed
He,
Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis.
2007,
Pubmed
He,
The glomerular transcriptome and a predicted protein-protein interaction network.
2008,
Pubmed
Holzman,
Nephrin localizes to the slit pore of the glomerular epithelial cell.
1999,
Pubmed
Ishikawa,
Mouse Nkd1, a Wnt antagonist, exhibits oscillatory gene expression in the PSM under the control of Notch signaling.
2004,
Pubmed
Kramer-Zucker,
Organization of the pronephric filtration apparatus in zebrafish requires Nephrin, Podocin and the FERM domain protein Mosaic eyes.
2005,
Pubmed
,
Xenbase
Mangos,
Fishing for new glomerular disease-related genes.
2011,
Pubmed
Miazek,
Two genes, three messengers: hybrid transcript between a gene expressed at specific stages of T-cell and sperm maturation and an unrelated adjacent gene.
2003,
Pubmed
Munton,
Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
2007,
Pubmed
Nagata,
Cell cycle regulation and differentiation in the human podocyte lineage.
1998,
Pubmed
Nilsson,
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.
2005,
Pubmed
Patrakka,
Expression and subcellular distribution of novel glomerulus-associated proteins dendrin, ehd3, sh2d4a, plekhh2, and 2310066E14Rik.
2007,
Pubmed
Roselli,
Podocin localizes in the kidney to the slit diaphragm area.
2002,
Pubmed
Sun,
Glomerular transcriptome changes associated with lipopolysaccharide-induced proteinuria.
2009,
Pubmed
Takemoto,
A new method for large scale isolation of kidney glomeruli from mice.
2002,
Pubmed
Takemoto,
Large-scale identification of genes implicated in kidney glomerulus development and function.
2006,
Pubmed
,
Xenbase
Tryggvason,
Hereditary proteinuria syndromes and mechanisms of proteinuria.
2006,
Pubmed
William,
Identification of oscillatory genes in somitogenesis from functional genomic analysis of a human mesenchymal stem cell model.
2007,
Pubmed
Zhou,
Proximo-distal specialization of epithelial transport processes within the Xenopus pronephric kidney tubules.
2004,
Pubmed
,
Xenbase