XB-ART-44419
Curr Protoc Pharmacol
2003 Jul 01;Chapter 11:Unit11.6. doi: 10.1002/0471141755.ph1106s21.
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Electrophysiological analysis of ATP-sensitive potassium channels in mammalian cells and Xenopus oocytes.
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This unit describes general methodologies for the characterization of ATP-sensitive K+ channels and the study of ligand-channel interactions in native tissues and clonal cell lines by electrophysiological techniques. Detailed protocols on how to establish patch-clamp single-channel and whole-cell current recording are presented. Two-electrode voltage clamp techniques for studying ATP-sensitive K+ channels expressed in Xenopus oocytes are also included.
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