Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Invert Neurosci
2003 Nov 01;51:29-35. doi: 10.1007/s10158-003-0025-1.
Show Gene links
Show Anatomy links
Action of nereistoxin on recombinant neuronal nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes.
Raymond Delpech V
,
Ihara M
,
Coddou C
,
Matsuda K
,
Sattelle DB
.
???displayArticle.abstract???
Nereistoxin (NTX), a natural neurotoxin from the salivary glands of the marine annelid worm Lumbriconereis heteropoda, is highly toxic to insects. Its synthetic analogue, Cartap, was the first commercial insecticide based on a natural product. We have used voltage-clamp electrophysiology to compare the actions of NTX on recombinant nicotinic acetylcholine receptors (nicotinic AChRs) expressed in Xenopus laevis oocytes following nuclear injection of cDNAs. The recombinant nicotinic AChRs investigated were chicken alpha7, chicken alpha4beta2 and the Drosophila melanogaster/chicken hybrid receptors SAD/beta2 and ALS/beta2. No agonist action of NTX (0.1-100 microM) was observed on chicken alpha7, chicken alpha4beta2 and the Drosophila/chicken hybrid nicotinic AChRs. Currents elicited by ACh were reduced in amplitude by NTX in a dose-dependent manner. The toxin was slightly more potent on recombinant Drosophila/vertebrate hybrid receptors than on vertebrate homomeric (alpha7) or heteromeric (alpha4beta2) nicotinic AChRs. Block by NTX of the chicken alpha7, chicken alpha4beta2 and the SAD/beta2 and ALS/beta2 Drosophila/chicken hybrid receptors is in all cases non-competitive. Thus, the site of action on nicotinic AChRs of NTX, to which the insecticide Cartap is metabolised in insects, differs from that of the major nicotinic AChR-active insecticide, imidacloprid.
Barnes,
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
1994, Pubmed
Barnes,
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
1994,
Pubmed
Bertrand,
Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate beta 2 subunit and Drosophila alpha subunits.
1994,
Pubmed
,
Xenbase
Blake,
Drosophila nervous system muscarinic acetylcholine receptor: transient functional expression and localization by immunocytochemistry.
1993,
Pubmed
,
Xenbase
Buckingham,
Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Eldefrawi,
Nereistoxin interaction with the acetylcholine receptor-ionic channel complex.
1980,
Pubmed
Grauso,
Novel putative nicotinic acetylcholine receptor subunit genes, Dalpha5, Dalpha6 and Dalpha7, in Drosophila melanogaster identify a new and highly conserved target of adenosine deaminase acting on RNA-mediated A-to-I pre-mRNA editing.
2002,
Pubmed
Gundelfinger,
How complex is the nicotinic receptor system of insects?
1992,
Pubmed
Lansdell,
Temperature-sensitive expression of Drosophila neuronal nicotinic acetylcholine receptors.
1997,
Pubmed
,
Xenbase
Littleton,
Ion channels and synaptic organization: analysis of the Drosophila genome.
2000,
Pubmed
Lupu-Meiri,
Extracellular calcium participates in responses to acetylcholine in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Matsuda,
Effects of the alpha subunit on imidacloprid sensitivity of recombinant nicotinic acetylcholine receptors.
1998,
Pubmed
,
Xenbase
Sattelle,
Functional genomics of ionotropic acetylcholine receptors in Caenorhabditis elegans and Drosophila melanogaster.
2002,
Pubmed
,
Xenbase
Sattelle,
Cholinergic nerve terminals in the central nervous system of insects.
1990,
Pubmed
Sawruk,
Heterogeneity of Drosophila nicotinic acetylcholine receptors: SAD, a novel developmentally regulated alpha-subunit.
1990,
Pubmed
,
Xenbase
Schulz,
D alpha3, a new functional alpha subunit of nicotinic acetylcholine receptors from Drosophila.
1998,
Pubmed
,
Xenbase
Swick,
Promoter-cDNA-directed heterologous protein expression in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Usherwood,
L-Glutamate at insect excitatory nerve-muscle synapses.
1968,
Pubmed
Xie,
Nereistoxin: a naturally occurring toxin with redox effects on neuronal nicotinic acetylcholine receptors in chick retina.
1993,
Pubmed
Xie,
Evidence that a nereistoxin metabolite, and not nereistoxin itself, reduces neuronal nicotinic receptors: studies in the whole chick ciliary ganglion, on isolated neurons and immunoprecipitated receptors.
1996,
Pubmed
