XB-ART-44137
Int J Antimicrob Agents
2011 Dec 01;386:510-5. doi: 10.1016/j.ijantimicag.2011.07.012.
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Isolation and characterisation of a new antimicrobial peptide from the skin of Xenopus laevis.
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A new antimicrobial peptide (AMP) named PGLa-H has been isolated from the skin of the African clawed frog (Xenopus laevis) using gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as KIAKVALKAL by Edman degradation, with a molecular weight of 1053.727 Da as analysed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). No similar AMP was found by BLAST search. Purified PGLa-H demonstrated antimicrobial ability against the reference bacteria Escherichia coli ATCC 25922 [minimum inhibitory concentration (MIC)=23.6 μg/mL], Staphylococcus aureus ATCC 25923 (MIC=8.7 μg/mL) and Bacillus subtilis (MIC=14.4μg/mL) and was active against multidrug-resistant meticillin-resistant S. aureus (MRSA) (MIC=67.8 μg/mL). The antimicrobial mechanism for this new peptide was further investigated by transmission electron microscopy. PGLa-H killed cells by destroying the cell membrane.
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Species referenced: Xenopus laevis
Genes referenced: pgla