XB-ART-43693
Methods Mol Biol
2011 Jan 01;725:281-93. doi: 10.1007/978-1-61779-046-1_18.
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Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs.
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Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
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Species referenced: Xenopus laevis
Genes referenced: piwil1