XB-ART-43598
J Nanosci Nanotechnol
2011 May 01;115:4596-600. doi: 10.1166/jnn.2011.3650.
Show Gene links
Show Anatomy links
Acetabularia rhodopsin I is a light-stimulated proton pump.
???displayArticle.abstract???
We cloned an intronless, nuclear-encoded opsin gene from an EST library of Acetabularia acetabulum. Acetabularia rhodopsin I (ARI) encodes a protein of 246 amino acids with molecular weight of 27 kDa. ARI was reconstituted in the Xenopus oocyte expression system to characterize its electrophysiological properties utilizing the two-electrode voltage-clamping technique. Oocytes where ARI cRNA was injected displayed outward directed currents in response to light. The maximum action spectrum of ARI was detected at 520 nm green light. Light-stimulated ARI current amplitude was altered by the protons, but not by the other ions in recording solutions, suggesting that the algal rhodopsin is a light-stimulated proton pump. Typical proton-mediated outward current elicited by 520 nm light was characterized with two phases of non-inactivating outward current following initial transient current. Taken together, we here reported cloning of a novel Acetabularia opsin gene which was characterized to be a proton-pump stimulated by light.
???displayArticle.pubmedLink??? 21780504
???displayArticle.link??? J Nanosci Nanotechnol
Species referenced: Xenopus
Genes referenced: rho