Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
PolyA-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma brucei.
Utter CJ
,
Garcia SA
,
Milone J
,
Bellofatto V
.
???displayArticle.abstract???
Deadenylation is often the rate-limiting event in regulating the turnover of cellular mRNAs in eukaryotes. Removal of the poly(A) tail initiates mRNA degradation by one of several decay pathways, including deadenylation-dependent decapping, followed by 5' to 3' exonuclease decay or 3' to 5' exosome-mediated decay. In trypanosomatids, mRNA degradation is important in controlling the expression of differentially expressed genes. Genomic annotation studies have revealed several potential deadenylases. Poly(A)-specific RNase (PARN) is a key deadenylase involved in regulating gene expression in mammals, Xenopus oocytes, and higher plants. Trypanosomatids possess three different PARN genes, PARN-1, -2, and -3, each of which is expressed at the mRNA level in two life-cycle stages of the human parasite Trypanosoma brucei. Here we show that T. brucei PARN-1 is an active deadenylase. To determine the role of PARN-1 on mRNA stability in vivo, we overexpressed this protein and analyzed perturbations in mRNA steady-state levels as well as mRNA half-life. Interestingly, a subset of mRNAs was affected, including a family of mRNAs that encode stage-specific coat proteins. These data suggest that PARN-1 functions in stage-specific protein production.
Balatsos,
Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC).
2006, Pubmed
Balatsos,
Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC).
2006,
Pubmed
Banerjee,
Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins.
2009,
Pubmed
Belostotsky,
Kill the messenger: mRNA decay and plant development.
2009,
Pubmed
Berberof,
The 3'-terminal region of the mRNAs for VSG and procyclin can confer stage specificity to gene expression in Trypanosoma brucei.
1995,
Pubmed
Berriman,
The genome of the African trypanosome Trypanosoma brucei.
2005,
Pubmed
Blattner,
The 3'-untranslated regions from the Trypanosoma brucei phosphoglycerate kinase-encoding genes mediate developmental regulation.
1995,
Pubmed
Brewer,
An A + U-rich element RNA-binding factor regulates c-myc mRNA stability in vitro.
1991,
Pubmed
Chen,
AU-rich elements: characterization and importance in mRNA degradation.
1995,
Pubmed
Chiba,
AtPARN is an essential poly(A) ribonuclease in Arabidopsis.
2004,
Pubmed
Chou,
Tethering KSRP, a decay-promoting AU-rich element-binding protein, to mRNAs elicits mRNA decay.
2006,
Pubmed
Clayton,
Life without transcriptional control? From fly to man and back again.
2002,
Pubmed
Das,
The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei.
2009,
Pubmed
Daugeron,
The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation.
2001,
Pubmed
Dehlin,
Cap-dependent deadenylation of mRNA.
2000,
Pubmed
,
Xenbase
Dlakić,
Functionally unrelated signalling proteins contain a fold similar to Mg2+-dependent endonucleases.
2000,
Pubmed
Estévez,
The exosome of Trypanosoma brucei.
2001,
Pubmed
Ford,
ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.
1999,
Pubmed
Ford,
An in vitro system using HeLa cytoplasmic extracts that reproduces regulated mRNA stability.
1999,
Pubmed
Fritz,
Messenger RNA decay in mammalian cells: the exonuclease perspective.
2004,
Pubmed
Furger,
Elements in the 3' untranslated region of procyclin mRNA regulate expression in insect forms of Trypanosoma brucei by modulating RNA stability and translation.
1997,
Pubmed
Gao,
Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro.
2000,
Pubmed
Garneau,
The highways and byways of mRNA decay.
2007,
Pubmed
Goldstrohm,
Regulated deadenylation in vitro.
2008,
Pubmed
Haile,
A role for the exosome in the in vivo degradation of unstable mRNAs.
2003,
Pubmed
Hirumi,
Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers.
1989,
Pubmed
Hotz,
Role of 3'-untranslated regions in the regulation of hexose transporter mRNAs in Trypanosoma brucei.
1995,
Pubmed
Hotz,
Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3'-untranslated region of a surface protein mRNA affects RNA abundance and translation.
1997,
Pubmed
Ibrahim,
Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei.
2009,
Pubmed
Jensen,
Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei.
2009,
Pubmed
Kim,
Opposing polymerase-deadenylase activities regulate cytoplasmic polyadenylation.
2006,
Pubmed
,
Xenbase
Kramer,
The RNA helicase DHH1 is central to the correct expression of many developmentally regulated mRNAs in trypanosomes.
2010,
Pubmed
Körner,
The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Lai,
Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease.
2003,
Pubmed
Lai,
Evidence that tristetraprolin binds to AU-rich elements and promotes the deadenylation and destabilization of tumor necrosis factor alpha mRNA.
1999,
Pubmed
Lejeune,
Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities.
2003,
Pubmed
Li,
Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation.
2006,
Pubmed
Martînez,
The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation.
2001,
Pubmed
Mauxion,
The BTG2 protein is a general activator of mRNA deadenylation.
2008,
Pubmed
Meyer,
Messenger RNA turnover in eukaryotes: pathways and enzymes.
2004,
Pubmed
Milone,
Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts.
2002,
Pubmed
Milone,
Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p.
2004,
Pubmed
Nilsson,
A multifunctional RNA recognition motif in poly(A)-specific ribonuclease with cap and poly(A) binding properties.
2007,
Pubmed
Nolan,
Characterization of a novel alanine-rich protein located in surface microdomains in Trypanosoma brucei.
2000,
Pubmed
Panigrahi,
Isolation and compositional analysis of trypanosomatid editosomes.
2007,
Pubmed
Parker,
The enzymes and control of eukaryotic mRNA turnover.
2004,
Pubmed
Peng,
RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein.
1998,
Pubmed
Reverdatto,
mRNA deadenylation by PARN is essential for embryogenesis in higher plants.
2004,
Pubmed
,
Xenbase
Schwede,
The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.
2009,
Pubmed
Schwede,
A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells.
2008,
Pubmed
Tadauchi,
Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF.
2001,
Pubmed
Tetaud,
Kinetoplastid glucose transporters.
1997,
Pubmed
Urwyler,
A family of stage-specific alanine-rich proteins on the surface of epimastigote forms of Trypanosoma brucei.
2007,
Pubmed
Vlasova,
Posttranscriptional regulation of gene networks by GU-rich elements and CELF proteins.
2008,
Pubmed
Wiederhold,
Cytoplasmic deadenylation: regulation of mRNA fate.
2010,
Pubmed
Wirtz,
A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei.
1999,
Pubmed
Yamashita,
Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover.
2005,
Pubmed
Zuo,
Exoribonuclease superfamilies: structural analysis and phylogenetic distribution.
2001,
Pubmed