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Effects of histone acetylation by Piccolo NuA4 on the structure of a nucleosome and the interactions between two nucleosomes.
Lee JY
,
Wei S
,
Lee TH
.
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We characterized the effect of histone acetylation on the structure of a nucleosome and the interactions between two nucleosomes. In this study, nucleosomes reconstituted with the Selex "Widom 601" sequence were acetylated with the Piccolo NuA4 complex, which acetylates mainly H4 N-terminal tail lysine residues and some H2A/H3 N-terminal tail lysine residues. Upon the acetylation, we observed directional unwrapping of nucleosomal DNA that accompanies topology change of the DNA. Interactions between two nucleosomes in solution were also monitored to discover multiple transient dinucleosomal states that can be categorized to short-lived and long-lived (∼1 s) states. The formation of dinucleosomes is strongly Mg(2+)-dependent, and unacetylated nucleosomes favor the formation of long-lived dinucleosomes 4-fold as much as the acetylated ones. These results suggest that the acetylation of histones by Piccolo NuA4 disturbs not only the structure of a nucleosome but also the interactions between two nucleosomes. Lastly, we suggest a structural model for a stable dinucleosomal state where the two nucleosomes are separated by ∼2 nm face-to-face and rotated by 34° with respect to each other.
ALLFREY,
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
1964, Pubmed
ALLFREY,
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
1964,
Pubmed
Anderson,
Effects of histone acetylation on the equilibrium accessibility of nucleosomal DNA target sites.
2001,
Pubmed
Arya,
A tale of tails: how histone tails mediate chromatin compaction in different salt and linker histone environments.
2009,
Pubmed
Ausio,
Histone hyperacetylation: its effects on nucleosome conformation and stability.
1986,
Pubmed
Banères,
The N tails of histones H3 and H4 adopt a highly structured conformation in the nucleosome.
1997,
Pubmed
Bauer,
Nucleosome structural changes due to acetylation.
1994,
Pubmed
,
Xenbase
Berndsen,
Assays for mechanistic investigations of protein/histone acetyltransferases.
2005,
Pubmed
Berndsen,
Nucleosome recognition by the Piccolo NuA4 histone acetyltransferase complex.
2007,
Pubmed
,
Xenbase
Bertin,
H3 and H4 histone tails play a central role in the interactions of recombinant NCPs.
2007,
Pubmed
Boudreault,
Yeast enhancer of polycomb defines global Esa1-dependent acetylation of chromatin.
2003,
Pubmed
Bruno,
Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities.
2003,
Pubmed
,
Xenbase
Choi,
Histone acetylation: truth of consequences?
2009,
Pubmed
Choy,
DNA methylation increases nucleosome compaction and rigidity.
2010,
Pubmed
Claudet,
Histone octamer instability under single molecule experiment conditions.
2005,
Pubmed
Cosgrove,
Regulated nucleosome mobility and the histone code.
2004,
Pubmed
Cosgrove,
How does the histone code work?
2005,
Pubmed
Dorigo,
Chromatin fiber folding: requirement for the histone H4 N-terminal tail.
2003,
Pubmed
,
Xenbase
Dorigo,
Nucleosome arrays reveal the two-start organization of the chromatin fiber.
2004,
Pubmed
,
Xenbase
Durrin,
Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.
1991,
Pubmed
Felsenfeld,
Controlling the double helix.
2003,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Fischle,
Histone and chromatin cross-talk.
2003,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Gansen,
Structural variability of nucleosomes detected by single-pair Förster resonance energy transfer: histone acetylation, sequence variation, and salt effects.
2009,
Pubmed
,
Xenbase
Gansen,
Nucleosome disassembly intermediates characterized by single-molecule FRET.
2009,
Pubmed
Gordon,
The core histone N-terminal tail domains function independently and additively during salt-dependent oligomerization of nucleosomal arrays.
2005,
Pubmed
,
Xenbase
Hansen,
Histone chaperones, histone acetylation, and the fluidity of the chromogenome.
2010,
Pubmed
Hansen,
Conformational dynamics of the chromatin fiber in solution: determinants, mechanisms, and functions.
2002,
Pubmed
Hebbes,
A direct link between core histone acetylation and transcriptionally active chromatin.
1988,
Pubmed
Kan,
The H4 tail domain participates in intra- and internucleosome interactions with protein and DNA during folding and oligomerization of nucleosome arrays.
2009,
Pubmed
Kimura,
Kinetics of core histones in living human cells: little exchange of H3 and H4 and some rapid exchange of H2B.
2001,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
The histone tails of the nucleosome.
1998,
Pubmed
Megee,
Histone H4 and the maintenance of genome integrity.
1995,
Pubmed
Norton,
Histone acetylation reduces nucleosome core particle linking number change.
1989,
Pubmed
Norton,
Nucleosome linking number change controlled by acetylation of histones H3 and H4.
1990,
Pubmed
Park,
Nucleosome assembly protein 1 exchanges histone H2A-H2B dimers and assists nucleosome sliding.
2005,
Pubmed
Pogo,
RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.
1966,
Pubmed
Poirier,
Dynamics and function of compact nucleosome arrays.
2009,
Pubmed
Robinson,
EM measurements define the dimensions of the "30-nm" chromatin fiber: evidence for a compact, interdigitated structure.
2006,
Pubmed
Robinson,
30 nm chromatin fibre decompaction requires both H4-K16 acetylation and linker histone eviction.
2008,
Pubmed
,
Xenbase
Schalch,
X-ray structure of a tetranucleosome and its implications for the chromatin fibre.
2005,
Pubmed
,
Xenbase
Selleck,
The Saccharomyces cerevisiae Piccolo NuA4 histone acetyltransferase complex requires the Enhancer of Polycomb A domain and chromodomain to acetylate nucleosomes.
2005,
Pubmed
Shahbazian,
Functions of site-specific histone acetylation and deacetylation.
2007,
Pubmed
Sinha,
Role of direct interactions between the histone H4 Tail and the H2A core in long range nucleosome contacts.
2010,
Pubmed
,
Xenbase
Sobel,
Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4.
1995,
Pubmed
Staynov,
The controversial 30 nm chromatin fibre.
2008,
Pubmed
Suka,
Sir2p and Sas2p opposingly regulate acetylation of yeast histone H4 lysine16 and spreading of heterochromatin.
2002,
Pubmed
Tan,
The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.
2005,
Pubmed
Tremethick,
Higher-order structures of chromatin: the elusive 30 nm fiber.
2007,
Pubmed
Tóth,
Association states of nucleosome assembly protein 1 and its complexes with histones.
2005,
Pubmed
Wang,
Acetylation increases the alpha-helical content of the histone tails of the nucleosome.
2000,
Pubmed
Zheng,
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
2003,
Pubmed
,
Xenbase
Zhou,
The nucleosome surface regulates chromatin compaction and couples it with transcriptional repression.
2007,
Pubmed
