XB-ART-43017
BMB Rep
2011 Mar 01;443:199-204. doi: 10.5483/BMBRep.2011.44.3.199.
Show Gene links
Show Anatomy links
EphrinB1 interacts with the transcriptional co-repressor Groucho/xTLE4.
???displayArticle.abstract???
Ephrin signaling is involved in various morphogenetic events, such as axon guidance, hindbrain segmentation, and angiogenesis. We conducted a yeast two-hybrid screen using the intracellular domain (ICD) of EphrinB1 to gain biochemical insightinto the function of the EphrinB1 ICD. We identified the transcriptional co-repressor xTLE1/Groucho as an EphrinB1 interacting protein. Whole-mount in situ hybridization of Xenopus embryos confirmed the co-localization of EphrinB1 and a Xenopus counterpart to TLE1, xTLE4, during various stages of development. The EphrinB1/xTLE4 interaction was confirmed by co-immunoprecipitation experiments. Further characterization of the interaction revealed that the carboxy-terminal PDZ binding motif of EphrinB1 and the SP domain of xTLE4 are required for binding. Additionally, phosphorylation of EphrinB1 by a constitutively activated fibroblast growth factor receptor resulted in loss of the interaction, suggesting that the interaction is modulated by tyrosine phosphorylation of the EphrinB1 ICD.
???displayArticle.pubmedLink??? 21429299
???displayArticle.link??? BMB Rep
Species referenced: Xenopus laevis
Genes referenced: efnb1 tle1 tle4