Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Cell
2004 Jan 01;161:99-113. doi: 10.1105/tpc.016865.
Show Gene links
Show Anatomy links
A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.
Stevens R
,
Grelon M
,
Vezon D
,
Oh J
,
Meyer P
,
Perennes C
,
Domenichini S
,
Bergounioux C
.
???displayArticle.abstract???
CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase alpha loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third of Arabidopsis 35S:CDC45 T1 RNA interference lines are partially to completely sterile, and the proportion of sterile plants is increased by using a dmc1 promoter. T1 plants have decreased levels of the CDC45 transcript and contain 21- to 23-bp RNA fragments specific to the CDC45 gene. T2 transgenic lines, in which small RNA fragments are still present, were used to analyze S-phase entry by 5-bromodeoxyuridine incorporation, which was not altered compared with that in the wild type. However, microarray data show that other cell cycle genes are upregulated or downregulated. T2 plants also have highly reduced fertility. The severity of the phenotype is correlated with the levels of the CDC45 transcript and small RNA fragments. Severe chromosome fragmentation arising during meiosis, which is not the result of a defect in the repair of SPO11-induced double strand breaks, leads to abnormal chromosome segregation and defective pollen and ovule development.
Alexander,
Differential staining of aborted and nonaborted pollen.
1969, Pubmed
Alexander,
Differential staining of aborted and nonaborted pollen.
1969,
Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Aparicio,
Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication.
1999,
Pubmed
Arata,
Cdk2-dependent and -independent pathways in E2F-mediated S phase induction.
2000,
Pubmed
Azumi,
Homolog interaction during meiotic prophase I in Arabidopsis requires the SOLO DANCERS gene encoding a novel cyclin-like protein.
2002,
Pubmed
Bergerat,
An atypical topoisomerase II from Archaea with implications for meiotic recombination.
1997,
Pubmed
Borde,
Direct coupling between meiotic DNA replication and recombination initiation.
2000,
Pubmed
Cha,
Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p.
2000,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Doutriaux,
Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana.
1998,
Pubmed
Edwards,
Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis.
1998,
Pubmed
Forsburg,
Only connect: linking meiotic DNA replication to chromosome dynamics.
2002,
Pubmed
Glab,
Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions.
1994,
Pubmed
Grelon,
AtSPO11-1 is necessary for efficient meiotic recombination in plants.
2001,
Pubmed
Grelon,
The Arabidopsis MEI1 gene encodes a protein with five BRCT domains that is involved in meiosis-specific DNA repair events independent of SPO11-induced DSBs.
2003,
Pubmed
Haber,
DNA recombination: the replication connection.
1999,
Pubmed
Hamilton,
A species of small antisense RNA in posttranscriptional gene silencing in plants.
1999,
Pubmed
Hardy,
Identification of Cdc45p, an essential factor required for DNA replication.
1997,
Pubmed
Hashimoto,
Xenopus Cut5 is essential for a CDK-dependent process in the initiation of DNA replication.
2003,
Pubmed
,
Xenbase
Helin,
Regulation of cell proliferation by the E2F transcription factors.
1998,
Pubmed
Himanen,
Auxin-mediated cell cycle activation during early lateral root initiation.
2002,
Pubmed
Hopwood,
Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication.
1996,
Pubmed
Hutvágner,
Detailed characterization of the posttranscriptional gene-silencing-related small RNA in a GUS gene-silenced tobacco.
2000,
Pubmed
Iino,
The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe.
1995,
Pubmed
Jasinski,
The CDK inhibitor NtKIS1a is involved in plant development, endoreduplication and restores normal development of cyclin D3; 1-overexpressing plants.
2002,
Pubmed
Keeney,
Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family.
1997,
Pubmed
Keeney,
Mechanism and control of meiotic recombination initiation.
2001,
Pubmed
Kelly,
Regulation of chromosome replication.
2000,
Pubmed
Klimyuk,
AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon-induced allelic variation and meiosis-associated expression.
1997,
Pubmed
Lindner,
Essential role of MCM proteins in premeiotic DNA replication.
2002,
Pubmed
Mariconti,
The E2F family of transcription factors from Arabidopsis thaliana. Novel and conserved components of the retinoblastoma/E2F pathway in plants.
2002,
Pubmed
Mimura,
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.
1998,
Pubmed
,
Xenbase
Mironov,
Cyclin-dependent kinases and cell division in plants- the nexus.
1999,
Pubmed
Moir,
Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies.
1982,
Pubmed
Murakami,
Regulation of premeiotic S phase and recombination-related double-strand DNA breaks during meiosis in fission yeast.
2001,
Pubmed
Nougarède,
Hierarchy of S-phase-promoting factors: yeast Dbf4-Cdc7 kinase requires prior S-phase cyclin-dependent kinase activation.
2000,
Pubmed
Ortega,
Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice.
2003,
Pubmed
Owens,
CDC45 is required in conjunction with CDC7/DBF4 to trigger the initiation of DNA replication.
1997,
Pubmed
Perennes,
Is arcA3 a possible mediator in the signal transduction pathway during agonist cell cycle arrest by salicylic acid and UV irradiation?
1999,
Pubmed
Reiser,
The Ovule and the Embryo Sac.
1993,
Pubmed
Ross,
A light microscopic atlas of meiosis in Arabidopsis thaliana.
1996,
Pubmed
Saha,
The human homolog of Saccharomyces cerevisiae CDC45.
1998,
Pubmed
Smith,
B-type cyclins CLB5 and CLB6 control the initiation of recombination and synaptonemal complex formation in yeast meiosis.
2001,
Pubmed
Stuart,
CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint.
1998,
Pubmed
Tercero,
DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.
2000,
Pubmed
Toone,
Getting started: regulating the initiation of DNA replication in yeast.
1997,
Pubmed
Tréhin,
Cloning of upstream sequences responsible for cell cycle regulation of the Nicotiana sylvestris CycB1;1 gene.
1997,
Pubmed
Vandepoele,
Genome-wide analysis of core cell cycle genes in Arabidopsis.
2002,
Pubmed
Wang,
Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology.
2000,
Pubmed
Waterhouse,
Gene silencing as an adaptive defence against viruses.
2001,
Pubmed
Wesley,
Construct design for efficient, effective and high-throughput gene silencing in plants.
2001,
Pubmed
Yang,
The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation.
1999,
Pubmed
Zamore,
RNA interference: listening to the sound of silence.
2001,
Pubmed
Zou,
Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase.
2000,
Pubmed
Zou,
Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin.
1998,
Pubmed
Zou,
CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication.
1997,
Pubmed
de Jager,
Retinoblastoma proteins in plants.
1999,
Pubmed
