Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleus
2010 Jan 01;16:487-91. doi: 10.4161/nucl.1.6.13514.
Show Gene links
Show Anatomy links
Cytosol-dependent membrane fusion in ER, nuclear envelope and nuclear pore assembly: biological implications.
Rafikova ER
,
Melikov K
,
Chernomordik LV
.
???displayArticle.abstract???
Endoplasmic reticulum and nuclear envelope rearrangements after mitosis are often studied in the reconstitution system based on Xenopus egg extract. In our recent work we partially replaced the membrane vesicles in the reconstitution mix with protein-free liposomes to explore the relative contributions of cytosolic and transmembrane proteins. Here we discuss our finding that cytosolic proteins mediate fusion between membranes lacking functional transmembrane proteins and the role of membrane fusion in endoplasmic reticulum and nuclear envelope reorganization. Cytosol-dependent liposome fusion has allowed us to restore, without adding transmembrane nucleoporins, functionality of nuclear pores, their spatial distribution and chromatin decondensation in nuclei formed at insufficient amounts of membrane material and characterized by only partial decondensation of chromatin and lack of nuclear transport. Both the mechanisms and the biological implications of the discovered coupling between spatial distribution of nuclear pores, chromatin decondensation and nuclear transport are discussed.
Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007, Pubmed,
Xenbase
Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007,
Pubmed
,
Xenbase
Baur,
NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts.
2007,
Pubmed
,
Xenbase
Chernomordik,
Mechanics of membrane fusion.
2008,
Pubmed
Chernomordik,
Protein-lipid interplay in fusion and fission of biological membranes.
2003,
Pubmed
Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed
Goldman,
Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome.
2004,
Pubmed
He,
Differential palmitoylation of the endosomal SNAREs syntaxin 7 and syntaxin 8.
2009,
Pubmed
Hetzer,
Border control at the nucleus: biogenesis and organization of the nuclear membrane and pore complexes.
2009,
Pubmed
Hu,
Membrane proteins of the endoplasmic reticulum induce high-curvature tubules.
2008,
Pubmed
Hu,
A class of dynamin-like GTPases involved in the generation of the tubular ER network.
2009,
Pubmed
Ishihara,
Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity.
2004,
Pubmed
Jahn,
SNAREs--engines for membrane fusion.
2006,
Pubmed
Langosch,
The role of transmembrane domains in membrane fusion.
2007,
Pubmed
Larijani,
Nuclear envelope formation: mind the gaps.
2009,
Pubmed
Lee,
Dynamic behavior of endoplasmic reticulum in living cells.
1988,
Pubmed
Leikina,
The 1-127 HA2 construct of influenza virus hemagglutinin induces cell-cell hemifusion.
2001,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lu,
Cisternal organization of the endoplasmic reticulum during mitosis.
2009,
Pubmed
Martens,
How synaptotagmin promotes membrane fusion.
2007,
Pubmed
Maul,
Formation and distribution of nuclear pore complexes in interphase.
1971,
Pubmed
McMahon,
Membrane curvature in synaptic vesicle fusion and beyond.
2010,
Pubmed
McNeil,
Requirement for annexin A1 in plasma membrane repair.
2006,
Pubmed
Melloy,
Distinct roles for key karyogamy proteins during yeast nuclear fusion.
2009,
Pubmed
Nakatogawa,
Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion.
2007,
Pubmed
Orso,
Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin.
2009,
Pubmed
Podbilewicz,
The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo.
2006,
Pubmed
Poteryaev,
Involvement of the actin cytoskeleton and homotypic membrane fusion in ER dynamics in Caenorhabditis elegans.
2005,
Pubmed
Puhka,
Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells.
2007,
Pubmed
Rafikova,
Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.
2009,
Pubmed
,
Xenbase
Ramos,
Transmembrane proteins are not required for early stages of nuclear envelope assembly.
2006,
Pubmed
Rasala,
Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.
2008,
Pubmed
,
Xenbase
Rotem,
Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly.
2009,
Pubmed
,
Xenbase
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Shen,
Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.
2009,
Pubmed
Skrahina,
Heterogeneity and timing of translocation and membrane-mediated assembly of different annexins.
2008,
Pubmed
Tomas,
Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope.
2003,
Pubmed
Verschure,
Condensed chromatin domains in the mammalian nucleus are accessible to large macromolecules.
2003,
Pubmed
Voeltz,
A class of membrane proteins shaping the tubular endoplasmic reticulum.
2006,
Pubmed
Vogel,
Targeting of SNAP-25 to membranes is mediated by its association with the target SNARE syntaxin.
2000,
Pubmed
Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase
Zimmerberg,
How proteins produce cellular membrane curvature.
2006,
Pubmed
