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Eukaryot Cell
2003 Dec 01;26:1220-33. doi: 10.1128/EC.2.6.1220-1233.2003.
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Molecular identification of a calcium-inhibited catalytic subunit of casein kinase type 2 from Paramecium tetraurelia.
Vetter D
,
Kissmehl R
,
Treptau T
,
Hauser K
,
Kellermann J
,
Plattner H
.
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We have previously described the occurrence in Paramecium of a casein kinase (CK) activity (EC 2.7.1.37) with some unusual properties, including inhibition by Ca(2+) (R. Kissmehl, T. Treptau, K. Hauser, and H. Plattner, FEBS Lett. 402:227-235, 1995). We now have cloned four genes, PtCK2alpha1 to PtCK2alpha4, all of which encode the catalytic alpha subunit of type 2 CK (CK2) with calculated molecular masses ranging from 38.9 to 39.4 kDa and pI values ranging from 8.8 to 9.0. They can be classified into two groups, which differ from each other by 28% on the nucleotide level and by 18% on the derived amino acid level. One of them, PtCK2alpha3, has been expressed in Escherichia coli and characterized in vitro. As we also have observed with the isolated CK, the recombinant protein preferentially phosphorylates casein but also phosphorylates some Paramecium-specific substrates, including the exocytosis-sensitive phosphoprotein pp63/parafusin. Characteristically, Ca(2+) inhibits the phosphorylation at elevated concentrations occurring during stimulation of a cell. Reconstitution with a recombinant form of the regulatory subunit from Xenopus laevis, XlCK2beta, confirms Ca(2+) sensitivity also under conditions of autophosphorylation. This is unusual for CK2 but correlates with the presence of two EF-hand calcium-binding motifs, one of which is located in the N-terminal segment essential for constitutive activity, as well as with an aberrant composition of normally basic domains recognizing acidic substrate domains. Immunogold localization reveals a considerable enrichment in the outermost cell cortex layers, excluding cilia. We discuss a potential role of this Ca(2+)-inhibited PtCK2alpha species in a late step of signal transduction.
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995, Pubmed
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995,
Pubmed
Bairoch,
The PROSITE database, its status in 1997.
1997,
Pubmed
Bers,
A practical guide to the preparation of Ca2+ buffers.
1994,
Pubmed
Carlson,
Isolation and characterization of protein kinases from Paramecium cilia.
1995,
Pubmed
Caron,
Does Paramecium primaurelia use a different genetic code in its macronucleus?
,
Pubmed
Cosmelli,
An inactive mutant of the alpha subunit of protein kinase CK2 that traps the regulatory CK2beta subunit.
1997,
Pubmed
,
Xenbase
Deng,
Site-directed mutagenesis of virtually any plasmid by eliminating a unique site.
1992,
Pubmed
Dessen,
Paramecium genome survey: a pilot project.
2001,
Pubmed
Dobrowolska,
CK2, a protein kinase of the next millennium.
1999,
Pubmed
Erxleben,
Microdomain Ca2+ activation during exocytosis in Paramecium cells. Superposition of local subplasmalemmal calcium store activation by local Ca2+ influx.
1997,
Pubmed
Fu,
Loss of the major isoform of phosphoglucomutase results in altered calcium homeostasis in Saccharomyces cerevisiae.
2000,
Pubmed
Glover,
On the physiological role of casein kinase II in Saccharomyces cerevisiae.
1998,
Pubmed
Gundersen,
A novel Ca2+-dependent protein kinase from Paramecium tetraurelia.
1987,
Pubmed
Hanks,
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
1995,
Pubmed
Hauser,
Molecular characterization of a sarco(endo)plasmic reticulum Ca2+-ATPase gene from Paramecium tetraurelia and localization of its gene product to sub-plasmalemmal calcium stores.
1998,
Pubmed
Hauser,
Green fluorescent protein-tagged sarco(endo)plasmic reticulum Ca2+-ATPase overexpression in Paramecium cells: isoforms, subcellular localization, biogenesis of cortical calcium stores and functional aspects.
2000,
Pubmed
Hauser,
Identification of isoforms of the exocytosis-sensitive phosphoprotein PP63/parafusin in Paramecium tetraurelia and demonstration of phosphoglucomutase activity.
1997,
Pubmed
Hinrichsen,
The regulation of calcium in Paramecium.
1995,
Pubmed
Hoekstra,
Budding and fission yeast casein kinase I isoforms have dual-specificity protein kinase activity.
1994,
Pubmed
Höhne-Zell,
A cortical phosphoprotein ('PP63') sensitive to exocytosis triggering in Paramecium cells. Immunolocalization and quenched-flow correlation of time course of dephosphorylation with membrane fusion.
1992,
Pubmed
Jedlicki,
The cDNAs coding for the alpha- and beta-subunits of Xenopus laevis casein kinase II.
1992,
Pubmed
,
Xenbase
Kawasaki,
Calcium-binding proteins 1: EF-hands.
1995,
Pubmed
Keryer,
Protein phosphorylation and dynamics of cytoskeletal structures associated with basal bodies in Paramecium.
1987,
Pubmed
Kim,
Ca2+-dependent protein kinases of Paramecium--cloning provides evidence of a multigene family.
1998,
Pubmed
Kissmehl,
Occurrence of a para-nitrophenyl phosphate-phosphatase with calcineurin-like characteristics in Paramecium tetraurelia.
1997,
Pubmed
Kissmehl,
Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein.
1998,
Pubmed
Kissmehl,
A novel, calcium-inhibitable casein kinase in Paramecium cells.
1997,
Pubmed
Kissmehl,
NSF regulates membrane traffic along multiple pathways in Paramecium.
2002,
Pubmed
Kissmehl,
Protein phosphatase and kinase activities possibly involved in exocytosis regulation in Paramecium tetraurelia.
1996,
Pubmed
Klauke,
Polyamine triggering of exocytosis in Paramecium involves an extracellular Ca(2+)/(polyvalent cation)-sensing receptor, subplasmalemmal Ca-store mobilization and store-operated Ca(2+)-influx via unspecific cation channels.
2000,
Pubmed
Klauke,
Imaging of Ca2+ transients induced in Paramecium cells by a polyamine secretagogue.
1997,
Pubmed
Kozak,
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
1984,
Pubmed
Kussmann,
Comparison of in vivo and in vitro phosphorylation of the exocytosis-sensitive protein PP63/parafusin by differential MALDI mass spectrometric peptide mapping.
1999,
Pubmed
Linder,
Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium.
1999,
Pubmed
Momayezi,
Exocytosis induction in Paramecium tetraurelia cells by exogenous phosphoprotein phosphatase in vivo and in vitro: possible involvement of calcineurin in exocytotic membrane fusion.
1987,
Pubmed
Momayezi,
Quantitative immunogold localization of protein phosphatase 2B (calcineurin) in Paramecium cells.
2000,
Pubmed
Momayezi,
Calmodulin in Paramecium tetraurelia: localization from the in vivo to the ultrastructural level.
1986,
Pubmed
Müller,
Crystal structure analysis of the exocytosis-sensitive phosphoprotein, pp63/parafusin (phosphoglucomutase), from Paramecium reveals significant conformational variability.
2002,
Pubmed
Padmanabha,
Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae.
1990,
Pubmed
Pinna,
Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation.
1997,
Pubmed
Plattner,
Electron microscopic methods in cellular and molecular biology.
1983,
Pubmed
Plattner,
Calcium in ciliated protozoa: sources, regulation, and calcium-regulated cell functions.
2001,
Pubmed
Plattner,
Stimulus-secretion coupling in Paramecium cells.
1991,
Pubmed
Prajer,
Dynamics of calcium regulation in Paramecium and possible morphogenetic implication.
1997,
Pubmed
Preer,
Deviation from the universal code shown by the gene for surface protein 51A in Paramecium.
,
Pubmed
Pulgar,
The recombinant alpha isoform of protein kinase CK1 from Xenopus laevis can phosphorylate tyrosine in synthetic substrates.
1996,
Pubmed
,
Xenbase
Ruiz,
Basal body duplication in Paramecium requires gamma-tubulin.
1999,
Pubmed
Russell,
Extremely short 20-33 nucleotide introns are the standard length in Paramecium tetraurelia.
1994,
Pubmed
Sarno,
Unique activation mechanism of protein kinase CK2. The N-terminal segment is essential for constitutive activity of the catalytic subunit but not of the holoenzyme.
2002,
Pubmed
Sarno,
Biochemical evidence that the N-terminal segments of the alpha subunit and the beta subunit play interchangeable roles in the activation of protein kinase CK2.
1998,
Pubmed
Satir,
The control of ciliary beat frequency.
1993,
Pubmed
Son,
A second member of the novel Ca(2+)-dependent protein kinase family from Paramecium tetraurelia. Purification and characterization.
1993,
Pubmed
Sperling,
Cortical morphogenesis in Paramecium: a transcellular wave of protein phosphorylation involved in ciliary rootlet disassembly.
1991,
Pubmed
Sperling,
Random sequencing of Paramecium somatic DNA.
2002,
Pubmed
Thalhofer,
Identification of two different phosphofructokinase-phosphorylating protein kinases from Ascaris suum muscle.
1988,
Pubmed
Van Houten,
Chemosensory transduction in eukaryotic microorganisms: trends for neuroscience?
1994,
Pubmed
Vayssié,
Molecular genetics of regulated secretion in paramecium.
2000,
Pubmed
Walczak,
Identification of a family of casein kinases in Paramecium: biochemical characterization and cellular localization.
1993,
Pubmed
Zieseniss,
Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains.
1985,
Pubmed