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Relevance of NAC-2, an Na+-coupled citrate transporter, to life span, body size and fat content in Caenorhabditis elegans.
Fei YJ
,
Liu JC
,
Inoue K
,
Zhuang L
,
Miyake K
,
Miyauchi S
,
Ganapathy V
.
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We have cloned and functionally characterized an Na+-coupled citrate transporter from Caenorhabditis elegans (ceNAC-2). This transporter shows significant sequence homology to Drosophila Indy and the mammalian Na+-coupled citrate transporter NaCT (now known as NaC2). When heterologously expressed in a mammalian cell line or in Xenopus oocytes, the cloned ceNAC-2 mediates the Na+-coupled transport of various intermediates of the citric acid cycle. However, it transports the tricarboxylate citrate more efficiently than dicarboxylates such as succinate, a feature different from that of ceNAC-1 (formerly known as ceNaDC1) and ceNAC-3 (formerly known as ceNaDC2). The transport process is electrogenic, as evidenced from the substrate-induced inward currents in oocytes expressing the transporter under voltage-clamp conditions. Expression studies using a reporter-gene fusion method in transgenic C. elegans show that the gene is expressed in the intestinal tract, the organ responsible for not only the digestion and absorption of nutrients but also for the storage of energy in this organism. Functional knockdown of the transporter by RNAi (RNA interference) not only leads to a significant increase in life span, but also causes a significant decrease in body size and fat content. The substrates of ceNAC-2 play a critical role in metabolic energy production and in the biosynthesis of cholesterol and fatty acids. The present studies suggest that the knockdown of these metabolic functions by RNAi is linked to an extension of life span and a decrease in fat content and body size.
Ashrafi,
Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes.
2003,
Pubmed
Chalfie,
Green fluorescent protein as a marker for gene expression.
1994,
Pubmed
Chen,
Characterization of a rat Na+-dicarboxylate cotransporter.
1998,
Pubmed
,
Xenbase
Chen,
Molecular and functional analysis of SDCT2, a novel rat sodium-dependent dicarboxylate transporter.
1999,
Pubmed
,
Xenbase
Fei,
Structural and functional characteristics of two sodium-coupled dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis elegans and their relevance to life span.
2003,
Pubmed
Fei,
Expression cloning of a mammalian proton-coupled oligopeptide transporter.
1994,
Pubmed
,
Xenbase
Fei,
A novel H(+)-coupled oligopeptide transporter (OPT3) from Caenorhabditis elegans with a predominant function as a H(+) channel and an exclusive expression in neurons.
2000,
Pubmed
,
Xenbase
Fei,
Two oligopeptide transporters from Caenorhabditis elegans: molecular cloning and functional expression.
1998,
Pubmed
,
Xenbase
Fire,
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
1998,
Pubmed
Greenspan,
Nile red: a selective fluorescent stain for intracellular lipid droplets.
1985,
Pubmed
Huang,
Transport of N-acetylaspartate by the Na(+)-dependent high-affinity dicarboxylate transporter NaDC3 and its relevance to the expression of the transporter in the brain.
2000,
Pubmed
Inoue,
Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates.
2002,
Pubmed
,
Xenbase
Inoue,
Structure, function, and expression pattern of a novel sodium-coupled citrate transporter (NaCT) cloned from mammalian brain.
2002,
Pubmed
Inoue,
Human Na+ -coupled citrate transporter: primary structure, genomic organization, and transport function.
2002,
Pubmed
Inoue,
Human sodium-coupled citrate transporter, the orthologue of Drosophila Indy, as a novel target for lithium action.
2003,
Pubmed
KREBS,
Chemical composition of blood plasma and serum.
1950,
Pubmed
Kekuda,
Primary structure and functional characteristics of a mammalian sodium-coupled high affinity dicarboxylate transporter.
1999,
Pubmed
Kenyon,
A C. elegans mutant that lives twice as long as wild type.
1993,
Pubmed
Knauf,
Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy.
2002,
Pubmed
,
Xenbase
Kramer,
The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen.
1990,
Pubmed
Lewis,
Basic culture methods.
1995,
Pubmed
Mackenzie,
The human intestinal H+/oligopeptide cotransporter hPEPT1 transports differently-charged dipeptides with identical electrogenic properties.
1996,
Pubmed
,
Xenbase
McCulloch,
Body size, insulin/IGF signaling and aging in the nematode Caenorhabditis elegans.
2003,
Pubmed
Mello,
DNA transformation.
1995,
Pubmed
Miller,
Immunofluorescence microscopy.
1995,
Pubmed
NORDMANN,
Organic acids in blood and urine.
1961,
Pubmed
Pajor,
Molecular cloning, chromosomal organization, and functional characterization of a sodium-dicarboxylate cotransporter from mouse kidney.
2000,
Pubmed
,
Xenbase
Pajor,
Sequence and functional characterization of a renal sodium/dicarboxylate cotransporter.
1995,
Pubmed
,
Xenbase
Pajor,
Functional differences between rabbit and human Na(+)-dicarboxylate cotransporters, NaDC-1 and hNaDC-1.
1996,
Pubmed
,
Xenbase
Rogina,
Extended life-span conferred by cotransporter gene mutations in Drosophila.
2000,
Pubmed
Sekine,
Cloning, functional characterization, and localization of a rat renal Na+-dicarboxylate transporter.
1998,
Pubmed
,
Xenbase
Timmons,
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.
2001,
Pubmed
Wang,
Structure, function, and genomic organization of human Na(+)-dependent high-affinity dicarboxylate transporter.
2000,
Pubmed
,
Xenbase
Wolkow,
Regulation of C. elegans life-span by insulinlike signaling in the nervous system.
2000,
Pubmed
