Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Mol Life Sci
2011 Sep 01;6817:2961-75. doi: 10.1007/s00018-010-0604-3.
Show Gene links
Show Anatomy links
Functional and structural determinants of reverse operation in the pH-dependent oligopeptide transporter PepT1.
Renna MD
,
Oyadeyi AS
,
Bossi E
,
Kottra G
,
Peres A
.
???displayArticle.abstract???
The functional and structural basis of reverse operation of PepT1 has been studied in Xenopus oocytes expressing the wild-type and mutated forms of this protein. Using brief pulses from a negative holding potential, wild-type and Arg282 mutants exhibit outward currents in the presence of Gly-Gln. The reversal potential of these currents is affected by both pH and substrate concentration, confirming coupled transport in the wild type and in the mutants as well. Long-lasting voltage and current-clamp experiments show that the outward currents are only temporary, and reflect accumulation and/or depletion effects near the membrane. The ability to operate in reverse mode was confirmed in all isoforms by intracellular injection of substrate. The role of Arg282 and Asp341 in the reverse transport was also investigated using charged substrates. Positive Lys-Gly (but not Gly-Lys) showed enhanced transport currents in the Arg282 mutants. In contrast, negative Gly-Asp and Asp-Gly elicited modest currents in all isoforms.
Abramson,
Structure and function of Na(+)-symporters with inverted repeats.
2009, Pubmed
Abramson,
Structure and function of Na(+)-symporters with inverted repeats.
2009,
Pubmed
Amasheh,
Transport of charged dipeptides by the intestinal H+/peptide symporter PepT1 expressed in Xenopus laevis oocytes.
1997,
Pubmed
,
Xenbase
Bossi,
Exogenous protein expression in Xenopus oocytes: basic procedures.
2007,
Pubmed
,
Xenbase
Chen,
Functional roles of histidine and tyrosine residues in the H(+)-peptide transporter PepT1.
2000,
Pubmed
,
Xenbase
Daniel,
From bacteria to man: archaic proton-dependent peptide transporters at work.
2006,
Pubmed
Fei,
Identification of the histidyl residue obligatory for the catalytic activity of the human H+/peptide cotransporters PEPT1 and PEPT2.
1997,
Pubmed
,
Xenbase
Fesce,
The relation between charge movement and transport-associated currents in the rat GABA cotransporter rGAT1.
2002,
Pubmed
,
Xenbase
HODGKIN,
The components of membrane conductance in the giant axon of Loligo.
1952,
Pubmed
Herrera-Ruiz,
Current perspectives on established and putative mammalian oligopeptide transporters.
2003,
Pubmed
Khoshbouei,
Amphetamine-induced dopamine efflux. A voltage-sensitive and intracellular Na+-dependent mechanism.
2003,
Pubmed
Knütter,
High-affinity interaction of sartans with H+/peptide transporters.
2009,
Pubmed
,
Xenbase
Kottra,
Bidirectional electrogenic transport of peptides by the proton-coupled carrier PEPT1 in Xenopus laevis oocytes: its asymmetry and symmetry.
2001,
Pubmed
,
Xenbase
Kottra,
Inhibition of intracellular dipeptide hydrolysis uncovers large outward transport currents of the peptide transporter PEPT1 in Xenopus oocytes.
2009,
Pubmed
,
Xenbase
Kottra,
PEPT1 as a paradigm for membrane carriers that mediate electrogenic bidirectional transport of anionic, cationic, and neutral substrates.
2002,
Pubmed
,
Xenbase
Krishnamurthy,
Unlocking the molecular secrets of sodium-coupled transporters.
2009,
Pubmed
Kulkarni,
Analysis of transmembrane segment 7 of the dipeptide transporter hPepT1 by cysteine-scanning mutagenesis.
2003,
Pubmed
Kulkarni,
A charge pair interaction between Arg282 in transmembrane segment 7 and Asp341 in transmembrane segment 8 of hPepT1.
2007,
Pubmed
McAlear,
Electrogenic Na/HCO3 cotransporter (NBCe1) variants expressed in Xenopus oocytes: functional comparison and roles of the amino and carboxy termini.
2006,
Pubmed
,
Xenbase
Meredith,
Molecular modeling of PepT1--towards a structure.
2006,
Pubmed
Meredith,
Site-directed mutation of arginine 282 to glutamate uncouples the movement of peptides and protons by the rabbit proton-peptide cotransporter PepT1.
2004,
Pubmed
,
Xenbase
Meredith,
Review. The mammalian proton-coupled peptide cotransporter PepT1: sitting on the transporter-channel fence?
2009,
Pubmed
,
Xenbase
Mertl,
Substrate-induced changes in the density of peptide transporter PEPT1 expressed in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Pieri,
Site-directed mutagenesis of Arginine282 suggests how protons and peptides are co-transported by rabbit PepT1.
2008,
Pubmed
,
Xenbase
Rauh,
A mutation of the epithelial sodium channel associated with atypical cystic fibrosis increases channel open probability and reduces Na+ self inhibition.
2010,
Pubmed
,
Xenbase
Richerson,
Dynamic equilibrium of neurotransmitter transporters: not just for reuptake anymore.
2003,
Pubmed
Richerson,
Role of the GABA transporter in epilepsy.
2004,
Pubmed
Rossi,
Glutamate release in severe brain ischaemia is mainly by reversed uptake.
2000,
Pubmed
Rühl,
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system.
2005,
Pubmed
Sala-Rabanal,
Molecular interactions between dipeptides, drugs and the human intestinal H+ -oligopeptide cotransporter hPEPT1.
2006,
Pubmed
,
Xenbase
Sangaletti,
Functional expression of the oligopeptide transporter PepT1 from the sea bass (Dicentrarchus labrax).
2009,
Pubmed
,
Xenbase
Seidel,
Amphetamines take two to tango: an oligomer-based counter-transport model of neurotransmitter transport explores the amphetamine action.
2005,
Pubmed
Terada,
Identification of the histidine residues involved in substrate recognition by a rat H+/peptide cotransporter, PEPT1.
1996,
Pubmed
,
Xenbase
Verri,
Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter.
2003,
Pubmed
,
Xenbase
Vig,
Human PEPT1 pharmacophore distinguishes between dipeptide transport and binding.
2006,
Pubmed
Yeung,
Molecular identification of a role for tyrosine 167 in the function of the human intestinal proton- coupled dipeptide transporter (hPepT1).
1998,
Pubmed
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
