Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Visualization of inositol 1,4,5-trisphosphate receptors on the nuclear envelope outer membrane by freeze-drying and rotary shadowing for electron microscopy.
Cárdenas C
,
Escobar M
,
García A
,
Osorio-Reich M
,
Härtel S
,
Foskett JK
,
Franzini-Armstrong C
.
???displayArticle.abstract???
The receptors for the second messenger InsP(3) comprise a family of closely related ion channels that release Ca(2+) from intracellular stores, most prominently the endoplasmic reticulum and its extension into the nuclear envelope. The precise sub-cellular localization of InsP(3)Rs and the spatial relationships among them are important for the initiation, spatial and temporal properties and propagation of local and global Ca(2+) signals, but the spatial organization of InsP(3)Rs in Ca(2+) stores is poorly characterized. Using nuclei isolated from insect Sf9 cells and freeze-dry rotary shadowing, we have addressed this by directly visualizing the cytoplasmic domain of InsP(3)R located on the cytoplasmic side of the nuclear envelope. Identification of approximately 15 nm structures as the cytoplasmic domain of InsP(3)R was indirectly supported by a marked increase in their frequency after transient transfections with cDNAs for rat types 1 and 3 InsP(3)R, and directly confirmed by gold labeling either with heparin or a specific anti-InsP(3)R antibody. Over-expression of InsP(3)R did not result in the formation of arrays or clusters with channels touching each other. Gold-labeling suggests that the channel amino terminus resides near the center of the cytoplasmic tetrameric quaternary structure. The combination of nuclear isolation with freeze-drying and rotary shadow techniques allows direct visualization of InsP(3)Rs in native nuclear envelopes and can be used to determine their spatial distribution and density.
Bailly,
Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation.
1999, Pubmed
Bailly,
Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation.
1999,
Pubmed
Berridge,
Inositol trisphosphate and calcium signalling.
1993,
Pubmed
Berridge,
Calcium signalling: dynamics, homeostasis and remodelling.
2003,
Pubmed
Block,
Structural evidence for direct interaction between the molecular components of the transverse tubule/sarcoplasmic reticulum junction in skeletal muscle.
1988,
Pubmed
Bosanac,
Structural insights into the regulatory mechanism of IP3 receptor.
2004,
Pubmed
Chalmers,
Agonist-evoked inositol trisphosphate receptor (IP3R) clustering is not dependent on changes in the structure of the endoplasmic reticulum.
2006,
Pubmed
Cárdenas,
Nuclear inositol 1,4,5-trisphosphate receptors regulate local Ca2+ transients and modulate cAMP response element binding protein phosphorylation.
2005,
Pubmed
Duncan,
Differential inositol 1,4,5-trisphosphate receptor signaling in a neuronal cell line.
2007,
Pubmed
Ferguson,
Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study.
1984,
Pubmed
Ferguson,
Ordered arrays of Ca2+-ATPase on the cytoplasmic surface of isolated sarcoplasmic reticulum.
1985,
Pubmed
Ferreri-Jacobia,
Translational mobility of the type 3 inositol 1,4,5-trisphosphate receptor Ca2+ release channel in endoplasmic reticulum membrane.
2005,
Pubmed
Foskett,
Inositol trisphosphate receptor Ca2+ release channels.
2007,
Pubmed
Franzini-Armstrong,
Comparative ultrastructure of Ca2+ release units in skeletal and cardiac muscle.
1998,
Pubmed
Ghosh,
Competitive, reversible, and potent antagonism of inositol 1,4,5-trisphosphate-activated calcium release by heparin.
1988,
Pubmed
Hamada,
Three-dimensional rearrangements within inositol 1,4,5-trisphosphate receptor by calcium.
2003,
Pubmed
Hamada,
Two-state conformational changes in inositol 1,4,5-trisphosphate receptor regulated by calcium.
2002,
Pubmed
Hofmann,
Wolfram syndrome: structural and functional analyses of mutant and wild-type wolframin, the WFS1 gene product.
2003,
Pubmed
Ionescu,
Graded recruitment and inactivation of single InsP3 receptor Ca2+-release channels: implications for quantal [corrected] Ca2+release.
2006,
Pubmed
Isobe,
Three-dimensional immunogold localization of alpha-actinin within the cytoskeletal networks of cultured cardiac muscle and nonmuscle cells.
1988,
Pubmed
Jarnik,
Toward a more complete 3-D structure of the nuclear pore complex.
1991,
Pubmed
,
Xenbase
Jiang,
Three-dimensional structure of the type 1 inositol 1,4,5-trisphosphate receptor at 24 A resolution.
2002,
Pubmed
Kada,
Recognition force microscopy/spectroscopy of ion channels: applications to the skeletal muscle Ca2+ release channel (RYR1).
2001,
Pubmed
Katayama,
Native structure and arrangement of inositol-1,4,5-trisphosphate receptor molecules in bovine cerebellar Purkinje cells as studied by quick-freeze deep-etch electron microscopy.
1996,
Pubmed
Kusnier,
Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line.
2006,
Pubmed
Loesser,
A simple method for freeze-drying of macromolecules and macromolecular complexes.
1990,
Pubmed
Mak,
Novel regulation of calcium inhibition of the inositol 1,4,5-trisphosphate receptor calcium-release channel.
2003,
Pubmed
,
Xenbase
Mak,
Spontaneous channel activity of the inositol 1,4,5-trisphosphate (InsP3) receptor (InsP3R). Application of allosteric modeling to calcium and InsP3 regulation of InsP3R single-channel gating.
2003,
Pubmed
,
Xenbase
Mak,
Single-channel inositol 1,4,5-trisphosphate receptor currents revealed by patch clamp of isolated Xenopus oocyte nuclei.
1994,
Pubmed
,
Xenbase
Malviya,
Stereospecific inositol 1,4,5-[32P]trisphosphate binding to isolated rat liver nuclei: evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus.
1990,
Pubmed
Marchenko,
Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurones.
2005,
Pubmed
Miyawaki,
Structure-function relationships of the mouse inositol 1,4,5-trisphosphate receptor.
1991,
Pubmed
Morita,
Visualization of inositol 1,4,5-trisphosphate receptor type III with green fluorescent protein in living cells.
2002,
Pubmed
Nakanishi,
Immunohistochemical localization of inositol 1,4,5-trisphosphate receptors in non-neural tissues, with special reference to epithelia, the reproductive system, and muscular tissues.
1996,
Pubmed
Newton,
Co-expression in vertebrate tissues and cell lines of multiple inositol 1,4,5-trisphosphate (InsP3) receptors with distinct affinities for InsP3.
1994,
Pubmed
Nosyreva,
The high-affinity calcium[bond]calmodulin-binding site does not play a role in the modulation of type 1 inositol 1,4,5-trisphosphate receptor function by calcium and calmodulin.
2002,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Paolini,
The relative position of RyR feet and DHPR tetrads in skeletal muscle.
2004,
Pubmed
Petersen,
The endoplasmic reticulum: one continuous or several separate Ca(2+) stores?
2001,
Pubmed
Pinto da Silva,
Label-fracture: a method for high resolution labeling of cell surfaces.
1984,
Pubmed
Rash,
Direct immunogold labeling of connexins and aquaporin-4 in freeze-fracture replicas of liver, brain, and spinal cord: factors limiting quantitative analysis.
1999,
Pubmed
Rash,
Freeze-fracture and immunogold analysis of aquaporin-4 (AQP4) square arrays, with models of AQP4 lattice assembly.
2004,
Pubmed
Serysheva,
Structure of the type 1 inositol 1,4,5-trisphosphate receptor revealed by electron cryomicroscopy.
2003,
Pubmed
Shuai,
The number and spatial distribution of IP3 receptors underlying calcium puffs in Xenopus oocytes.
2006,
Pubmed
,
Xenbase
Shuai,
A kinetic model of single and clustered IP3 receptors in the absence of Ca2+ feedback.
2007,
Pubmed
,
Xenbase
Smith,
Imaging the quantal substructure of single IP3R channel activity during Ca2+ puffs in intact mammalian cells.
2009,
Pubmed
Sugawara,
Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5-trisphosphate receptors in signal transduction through the B-cell antigen receptor.
1997,
Pubmed
Suhara,
Visualization of inositol 1,4,5-trisphosphate receptor by atomic force microscopy.
2006,
Pubmed
Supattapone,
Solubilization, purification, and characterization of an inositol trisphosphate receptor.
1988,
Pubmed
Takei,
Inositol 1,4,5-trisphosphate receptor causes formation of ER cisternal stacks in transfected fibroblasts and in cerebellar Purkinje cells.
1994,
Pubmed
Takekura,
Co-expression in CHO cells of two muscle proteins involved in excitation-contraction coupling.
1995,
Pubmed
Tateishi,
Cluster formation of inositol 1,4,5-trisphosphate receptor requires its transition to open state.
2005,
Pubmed
Taufiq-Ur-Rahman,
Clustering of InsP3 receptors by InsP3 retunes their regulation by InsP3 and Ca2+.
2009,
Pubmed
Taylor,
IP(3) receptors: the search for structure.
2004,
Pubmed
Tojyo,
The clustering of inositol 1,4,5-trisphosphate (IP(3)) receptors is triggered by IP(3) binding and facilitated by depletion of the Ca(2+) store.
2008,
Pubmed
Tu,
Functional characterization of mammalian inositol 1,4,5-trisphosphate receptor isoforms.
2005,
Pubmed
Vermassen,
Subcellular distribution of the inositol 1,4,5-trisphosphate receptors: functional relevance and molecular determinants.
2004,
Pubmed
Wilson,
Calcium-dependent clustering of inositol 1,4,5-trisphosphate receptors.
1998,
Pubmed
Wu,
Local InsP3-dependent perinuclear Ca2+ signaling in cardiac myocyte excitation-transcription coupling.
2006,
Pubmed
Yamamoto,
Stacks of flattened smooth endoplasmic reticulum highly enriched in inositol 1,4,5-trisphosphate (InsP3) receptor in mouse cerebellar Purkinje cells.
1991,
Pubmed
Yin,
Intrinsic lattice formation by the ryanodine receptor calcium-release channel.
2000,
Pubmed
Yin,
Ryanodine receptor arrays: not just a pretty pattern?
2008,
Pubmed
Zima,
IP3-dependent nuclear Ca2+ signalling in the mammalian heart.
2007,
Pubmed
da Fonseca,
Domain organization of the type 1 inositol 1,4,5-trisphosphate receptor as revealed by single-particle analysis.
2003,
Pubmed
