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J Biol Chem
2010 Dec 17;28551:39710-7. doi: 10.1074/jbc.M110.141457.
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Functional properties of the Arabidopsis peptide transporters AtPTR1 and AtPTR5.
Hammes UZ
,
Meier S
,
Dietrich D
,
Ward JM
,
Rentsch D
.
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The Arabidopsis di- and tripeptide transporters AtPTR1 and AtPTR5 were expressed in Xenopus laevis oocytes, and their selectivity and kinetic properties were determined by voltage clamping and by radioactive uptake. Dipeptide transport by AtPTR1 and AtPTR5 was found to be electrogenic and dependent on protons but not sodium. In the absence of dipeptides, both transporters showed proton-dependent leak currents that were inhibited by Phe-Ala (AtPTR5) and Phe-Ala, Trp-Ala, and Phe-Phe (AtPTR1). Phe-Ala was shown to reduce leak currents by binding to the substrate-binding site with a high apparent affinity. Inhibition of leak currents was only observed when the aromatic amino acids were present at the N-terminal position. AtPTR1 and AtPTR5 transport activity was voltage-dependent, and currents increased supralinearly with more negative membrane potentials and did not saturate. The voltage dependence of the apparent affinities differed between Ala-Ala, Ala-Lys, and Ala-Asp and was not conserved between the two transporters. The apparent affinity of AtPTR1 for these dipeptides was pH-dependent and decreased with decreasing proton concentration. In contrast to most proton-coupled transporters characterized so far, -I(max) increased at high pH, indicating that regulation of the transporter by pH overrides the importance of protons as co-substrate.
Baukrowitz,
Inward rectification in KATP channels: a pH switch in the pore.
1999, Pubmed
Baukrowitz,
Inward rectification in KATP channels: a pH switch in the pore.
1999,
Pubmed
Biegel,
The renal type H+/peptide symporter PEPT2: structure-affinity relationships.
2006,
Pubmed
Chen,
Functional roles of histidine and tyrosine residues in the H(+)-peptide transporter PepT1.
2000,
Pubmed
,
Xenbase
Chen,
Stoichiometry and kinetics of the high-affinity H+-coupled peptide transporter PepT2.
1999,
Pubmed
,
Xenbase
Chiang,
Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2.
2004,
Pubmed
,
Xenbase
Daniel,
The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology.
2004,
Pubmed
Daniel,
Molecular and integrative physiology of intestinal peptide transport.
2004,
Pubmed
Daniel,
Determinants of substrate affinity for the oligopeptide/H+ symporter in the renal brush border membrane.
1992,
Pubmed
Daniel,
From bacteria to man: archaic proton-dependent peptide transporters at work.
2006,
Pubmed
Detmers,
Peptides and ATP binding cassette peptide transporters.
2001,
Pubmed
Dietrich,
AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis.
2004,
Pubmed
,
Xenbase
Fang,
Kinetics and substrate specificity of membrane-reconstituted peptide transporter DtpT of Lactococcus lactis.
2000,
Pubmed
Fei,
Identification of the histidyl residue obligatory for the catalytic activity of the human H+/peptide cotransporters PEPT1 and PEPT2.
1997,
Pubmed
,
Xenbase
Jeong,
A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.
2004,
Pubmed
,
Xenbase
Komarova,
AtPTR1 and AtPTR5 transport dipeptides in planta.
2008,
Pubmed
,
Xenbase
Kottra,
PEPT1 as a paradigm for membrane carriers that mediate electrogenic bidirectional transport of anionic, cationic, and neutral substrates.
2002,
Pubmed
,
Xenbase
Mackenzie,
Mechanisms of the human intestinal H+-coupled oligopeptide transporter hPEPT1.
1996,
Pubmed
,
Xenbase
Meharg,
NO3- transport across the plasma membrane of Arabidopsis thaliana root hairs: kinetic control by pH and membrane voltage.
1995,
Pubmed
Miranda,
Peptide and amino acid transporters are differentially regulated during seed development and germination in faba bean.
2003,
Pubmed
Paungfoo-Lonhienne,
Nitrogen affects cluster root formation and expression of putative peptide transporters.
2009,
Pubmed
Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed
Rentsch,
Transporters for uptake and allocation of organic nitrogen compounds in plants.
2007,
Pubmed
Saier,
A functional-phylogenetic classification system for transmembrane solute transporters.
2000,
Pubmed
Segonzac,
Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter.
2007,
Pubmed
Sivitz,
Arabidopsis sucrose transporter AtSUC9. High-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype.
2007,
Pubmed
,
Xenbase
Song,
Antisense expression of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants.
1997,
Pubmed
Sugiura,
A nitrite transporter associated with nitrite uptake by higher plant chloroplasts.
2007,
Pubmed
Tsay,
Nitrate transporters and peptide transporters.
2007,
Pubmed
Ward,
Patch-clamping and other molecular approaches for the study of plasma membrane transporters demystified.
1997,
Pubmed
Waterworth,
Enigma variations for peptides and their transporters in higher plants.
2006,
Pubmed
West,
Cloning and functional characterisation of a peptide transporter expressed in the scutellum of barley grain during the early stages of germination.
1998,
Pubmed
,
Xenbase
Zhou,
Cloning and functional characterization of a Brassica napus transporter that is able to transport nitrate and histidine.
1998,
Pubmed
,
Xenbase
