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Pflugers Arch
2011 Jan 01;4611:65-75. doi: 10.1007/s00424-010-0887-0.
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Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat.
Poulsen AN
,
Jansen-Olesen I
,
Olesen J
,
Klaerke DA
.
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The big conductance calcium-activated K(+) channel (BK) is involved in regulating neuron and smooth muscle cell excitability. Functional diversity of BK is generated by alpha-subunit splice variation and co-expression with beta subunits. Here, we present six different splice combinations cloned from rat brain or cerebral vascular/meningeal tissues, of which at least three variants were previously uncharacterized (X1, X2(92), and X2(188)). An additional variant was identified by polymerase chain reaction but not cloned. Expression in Xenopus oocytes showed that the brain-specific X1 variant displays reduced current, faster activation, and less voltage sensitivity than the insert-less Zero variant. Other cloned variants Strex and Slo27,3 showed slower activation than Zero. The X1 variant contains sequence inserts in the S1-S2 extracellular loop (8 aa), between intracellular domains RCK1 and RCK2 (4 aa at SS1) and upstream of the calcium "bowl" (27 aa at SS4). Two other truncated variants, X2(92) and X2(188), lacking the intracellular C-terminal (stop downstream of S6), were cloned from cerebral vascular/meningeal tissue. They appear non-functional as no current expression was observed, but the X2(92) appeared to slow the activation of the Zero variant when co-expressed. Positive protein expression of X2(92) was observed in oocytes by immunoblotting and fluorescence using a yellow fluorescent protein-tagged construct. The functional characteristics of the X1 variant may be important for a subpopulation of BK channels in the brain, while the "silent" truncated variants X2(92) and X2(188) may play a role as modulators of other BK channel variants in a way similar to well known beta subunits.
Bravo-Zehnder,
Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.
2000, Pubmed
Bravo-Zehnder,
Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.
2000,
Pubmed
Brayden,
Regulation of arterial tone by activation of calcium-dependent potassium channels.
1992,
Pubmed
Brown,
A step-by-step guide to non-linear regression analysis of experimental data using a Microsoft Excel spreadsheet.
2001,
Pubmed
Dworetzky,
Cloning and expression of a human large-conductance calcium-activated potassium channel.
1994,
Pubmed
,
Xenbase
Fettiplace,
Mechanisms of hair cell tuning.
1999,
Pubmed
Fodor,
Convergent evolution of alternative splices at domain boundaries of the BK channel.
2009,
Pubmed
Geu-Flores,
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products.
2007,
Pubmed
Giraldez,
Generation of functional fluorescent BK channels by random insertion of GFP variants.
2005,
Pubmed
Grunnet,
Apamin interacts with all subtypes of cloned small-conductance Ca2+-activated K+ channels.
2001,
Pubmed
,
Xenbase
Gu,
BK potassium channels facilitate high-frequency firing and cause early spike frequency adaptation in rat CA1 hippocampal pyramidal cells.
2007,
Pubmed
Ha,
Functional characteristics of two BKCa channel variants differentially expressed in rat brain tissues.
2000,
Pubmed
,
Xenbase
Jiang,
Crystal structure and mechanism of a calcium-gated potassium channel.
2002,
Pubmed
Jones,
The role of Ca2+-activated K+ channel spliced variants in the tonotopic organization of the turtle cochlea.
1999,
Pubmed
,
Xenbase
Kim,
Alternatively spliced C-terminal domains regulate the surface expression of large conductance calcium-activated potassium channels.
2007,
Pubmed
Krause,
Xenopus laevis oocytes contain endogenous large conductance Ca2(+)-activated K+ channels.
1996,
Pubmed
,
Xenbase
Lee,
Modulation of the conductance-voltage relationship of the BK(Ca) channel by shortening the cytosolic loop connecting two RCK domains.
2009,
Pubmed
Li,
Characterization of voltage-and Ca2+-activated K+ channels in rat dorsal root ganglion neurons.
2007,
Pubmed
,
Xenbase
Limón,
Ca2+-activated K+-current density is correlated with soma size in rat vestibular-afferent neurons in culture.
2005,
Pubmed
Liu,
Locations of the beta1 transmembrane helices in the BK potassium channel.
2008,
Pubmed
Loane,
Co-assembly of N-type Ca2+ and BK channels underlies functional coupling in rat brain.
2007,
Pubmed
Marty,
Three types of calcium-dependent channel in rat lacrimal glands.
1984,
Pubmed
McCartney,
A cysteine-rich motif confers hypoxia sensitivity to mammalian large conductance voltage- and Ca-activated K (BK) channel alpha-subunits.
2005,
Pubmed
Naruse,
Stress-Axis Regulated Exon (STREX) in the C terminus of BK(Ca) channels is responsible for the stretch sensitivity.
2009,
Pubmed
Nour-Eldin,
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
2006,
Pubmed
Orio,
New disguises for an old channel: MaxiK channel beta-subunits.
2002,
Pubmed
Parker,
Ca2+ transients associated with openings of inositol trisphosphate-gated channels in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Petrik,
Regulation of STREX exon large conductance, calcium-activated potassium channels by the beta4 accessory subunit.
2007,
Pubmed
Pietrzykowski,
Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol.
2008,
Pubmed
Piskorowski,
Calcium activation of BK(Ca) potassium channels lacking the calcium bowl and RCK domains.
2002,
Pubmed
,
Xenbase
Poulsen,
The KCNE1 beta-subunit exerts a transient effect on the KCNQ1 K+ channel.
2007,
Pubmed
,
Xenbase
Poulsen,
Differential expression of BK channel isoforms and beta-subunits in rat neuro-vascular tissues.
2009,
Pubmed
,
Xenbase
Quirk,
Identification of a novel tetramerization domain in large conductance K(ca) channels.
2001,
Pubmed
,
Xenbase
Ramanathan,
beta subunits modulate alternatively spliced, large conductance, calcium-activated potassium channels of avian hair cells.
2000,
Pubmed
Rosenblatt,
Distribution of Ca2+-activated K+ channel isoforms along the tonotopic gradient of the chicken's cochlea.
1997,
Pubmed
Saito,
A cysteine-rich domain defined by a novel exon in a slo variant in rat adrenal chromaffin cells and PC12 cells.
1997,
Pubmed
,
Xenbase
Schmalhofer,
Role of the C-terminus of the high-conductance calcium-activated potassium channel in channel structure and function.
2005,
Pubmed
Shipston,
Alternative splicing of potassium channels: a dynamic switch of cellular excitability.
2001,
Pubmed
Shoudai,
Effects of various K+ channel blockers on spontaneous glycine release at rat spinal neurons.
2007,
Pubmed
Tian,
Distinct stoichiometry of BKCa channel tetramer phosphorylation specifies channel activation and inhibition by cAMP-dependent protein kinase.
2004,
Pubmed
Tian,
Palmitoylation gates phosphorylation-dependent regulation of BK potassium channels.
2008,
Pubmed
Torres,
A marriage of convenience: beta-subunits and voltage-dependent K+ channels.
2007,
Pubmed
Traub,
Fast rhythmic bursting can be induced in layer 2/3 cortical neurons by enhancing persistent Na+ conductance or by blocking BK channels.
2003,
Pubmed
Tseng-Crank,
Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain.
1994,
Pubmed
,
Xenbase
Wu,
An improved western blotting technique effectively reduces background.
2002,
Pubmed
Xie,
Control of alternative splicing of potassium channels by stress hormones.
1998,
Pubmed
,
Xenbase
Zou,
The beta 1 subunit of L-type voltage-gated Ca2+ channels independently binds to and inhibits the gating of large-conductance Ca2+-activated K+ channels.
2008,
Pubmed