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GEMC1 is a novel TopBP1-interacting protein involved in chromosomal DNA replication.
Piergiovanni G
,
Costanzo V
.
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Chromosomal DNA must be precisely replicated in each cell cycle in order to ensure maintenance of genome stability. Most of the factors controlling this process have been identified in lower eukaryotes. Several factors involved in DNA replication are also important for the cellular response to stress conditions. However, the regulation of DNA replication in multi-cellular organisms is still poorly understood. Using the Xenopus laevis egg cell-free system, we have recently identified a novel vertebrate protein named GEMC1 required for DNA replication. xGEMC1 is a Cyclin dependent kinase (CDK) target required forCdc45 loading onto chromatin and it interacts with the checkpoint and replication factor TopBP1, which promotes its binding to chromatin during prereplication complex formation. Here we discuss our recent findings and propose possible roles for GEMC1. Interestingly, recent studies have identified other proteins with analogous functions, showing a higher level of complexity in metazoan replication control compared to lower eukaryotes.
Aguilera,
Genome instability: a mechanistic view of its causes and consequences.
2008, Pubmed
Aguilera,
Genome instability: a mechanistic view of its causes and consequences.
2008,
Pubmed
Balestrini,
GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication.
2010,
Pubmed
,
Xenbase
Blow,
A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts.
1990,
Pubmed
,
Xenbase
Chowdhury,
The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation.
2010,
Pubmed
,
Xenbase
Diffley,
Regulation of early events in chromosome replication.
2004,
Pubmed
,
Xenbase
Errico,
Differences in the DNA replication of unicellular eukaryotes and metazoans: known unknowns.
2010,
Pubmed
Garcia,
Identification and functional analysis of TopBP1 and its homologs.
2005,
Pubmed
Garner,
Studying the DNA damage response using in vitro model systems.
2009,
Pubmed
,
Xenbase
Kamimura,
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.
2001,
Pubmed
Kamimura,
Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication.
1998,
Pubmed
Kumagai,
TopBP1 activates the ATR-ATRIP complex.
2006,
Pubmed
,
Xenbase
Kumagai,
Treslin collaborates with TopBP1 in triggering the initiation of DNA replication.
2010,
Pubmed
,
Xenbase
Labib,
How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?
2010,
Pubmed
Manke,
BRCT repeats as phosphopeptide-binding modules involved in protein targeting.
2003,
Pubmed
Matsuno,
The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase alpha in the initiation of DNA replication.
2006,
Pubmed
,
Xenbase
Muramatsu,
CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast.
2010,
Pubmed
Negrini,
Genomic instability--an evolving hallmark of cancer.
2010,
Pubmed
Sangrithi,
Initiation of DNA replication requires the RECQL4 protein mutated in Rothmund-Thomson syndrome.
2005,
Pubmed
,
Xenbase
Sansam,
A vertebrate gene, ticrr, is an essential checkpoint and replication regulator.
2010,
Pubmed
Saxena,
A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition.
2004,
Pubmed
,
Xenbase
Shechter,
ATR and ATM regulate the timing of DNA replication origin firing.
2004,
Pubmed
,
Xenbase
Swords,
Cdc7 kinase - a new target for drug development.
2010,
Pubmed
Tanaka,
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.
2007,
Pubmed
Tanaka,
The role of CDK in the initiation step of DNA replication in eukaryotes.
2007,
Pubmed
Xu,
MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.
2009,
Pubmed
,
Xenbase
Zegerman,
Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.
2007,
Pubmed
Zegerman,
DNA replication as a target of the DNA damage checkpoint.
2009,
Pubmed
