Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2004 Mar 01;153:1244-53. doi: 10.1091/mbc.e03-08-0600.
Show Gene links
Show Anatomy links
A cell-specific transgenic approach in Xenopus reveals the importance of a functional p24 system for a secretory cell.
Bouw G
,
Van Huizen R
,
Jansen EJ
,
Martens GJ
.
???displayArticle.abstract???
The p24alpha, -beta, -gamma, and -delta proteins are major multimeric constituents of cycling endoplasmic reticulum-Golgi transport vesicles and are thought to be involved in protein transport through the early secretory pathway. In this study, we targeted transgene overexpression of p24delta2 specifically to the Xenopus intermediate pituitarymelanotrope cell that is involved in background adaptation of the animal and produces high levels of its major secretory cargo proopiomelanocortin (POMC). The transgene product effectively displaced the endogenous p24 proteins, resulting in a melanotrope cell p24 system that consisted predominantly of the transgene p24delta2 protein. Despite the severely distorted p24 machinery, the subcellular structures as well as the level of POMC synthesis were normal in these cells. However, the number and pigment content of skin melanophores were reduced, impairing the ability of the transgenic animal to fully adapt to a black background. This physiological effect was likely caused by the affected profile of POMC-derived peptides observed in the transgenic melanotrope cells. Together, our results suggest that in the early secretory pathway an intact p24 system is essential for efficient secretory cargo transport or for supplying cargo carriers with the correct protein machinery to allow proper secretory protein processing.
Barlowe,
Traffic COPs of the early secretory pathway.
2000, Pubmed
Barlowe,
Traffic COPs of the early secretory pathway.
2000,
Pubmed
Barlowe,
COPII and selective export from the endoplasmic reticulum.
1998,
Pubmed
Belden,
Deletion of yeast p24 genes activates the unfolded protein response.
2001,
Pubmed
Berghs,
The secretory granule and pro-opiomelanocortin processing in Xenopus melanotrope cells during background adaptation.
1997,
Pubmed
,
Xenbase
Bicknell,
Characterization of a serine protease that cleaves pro-gamma-melanotropin at the adrenal to stimulate growth.
2001,
Pubmed
Blum,
Intracellular localization and in vivo trafficking of p24A and p23.
1999,
Pubmed
Braks,
7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway.
1994,
Pubmed
,
Xenbase
Bremser,
Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors.
1999,
Pubmed
Ciufo,
Identification of a lumenal sequence specifying the assembly of Emp24p into p24 complexes in the yeast secretory pathway.
2000,
Pubmed
Cuppen,
A FERM domain governs apical confinement of PTP-BL in epithelial cells.
1999,
Pubmed
Denzel,
The p24 family member p23 is required for early embryonic development.
2000,
Pubmed
Dominguez,
gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer.
1998,
Pubmed
Elrod-Erickson,
Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations.
1996,
Pubmed
Emery,
Coupled transport of p24 family members.
2000,
Pubmed
Fiedler,
Bimodal interaction of coatomer with the p24 family of putative cargo receptors.
1996,
Pubmed
Fransen,
Immuno-electronmicroscopical localization of a microvillus membrane disaccharidase in the human small-intestinal epithelium with monoclonal antibodies.
1985,
Pubmed
Füllekrug,
Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family members.
1999,
Pubmed
Hadley,
Biological actions of melanocyte-stimulating hormone.
1981,
Pubmed
Holthuis,
Molecular probing of the secretory pathway in peptide hormone-producing cells.
1995,
Pubmed
,
Xenbase
Holthuis,
Translocon-associated protein TRAP delta and a novel TRAP-like protein are coordinately expressed with pro-opiomelanocortin in Xenopus intermediate pituitary.
1995,
Pubmed
,
Xenbase
Jansen,
Transgene-driven protein expression specific to the intermediate pituitary melanotrope cells of Xenopus laevis.
2002,
Pubmed
,
Xenbase
Jenks,
Dual action of GABAA receptors on the secretory process of melanotrophs of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Jenne,
Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway.
2002,
Pubmed
Kaiser,
Thinking about p24 proteins and how transport vesicles select their cargo.
2000,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Kuiper,
Localization of p24 putative cargo receptors in the early secretory pathway depends on the biosynthetic activity of the cell.
2001,
Pubmed
,
Xenbase
Kuiper,
Differential induction of two p24delta putative cargo receptors upon activation of a prohormone-producing cell.
2000,
Pubmed
,
Xenbase
Lavoie,
Roles for alpha(2)p24 and COPI in endoplasmic reticulum cargo exit site formation.
1999,
Pubmed
Martens,
N alpha-acetylation is linked to alpha-MSH release from pars intermedia of the amphibian pituitary gland.
1981,
Pubmed
,
Xenbase
Martens,
Biosynthesis of two structurally different pro-opiomelanocortins in the pars intermedia of the amphibian pituitary gland.
1982,
Pubmed
,
Xenbase
Martens,
Biosynthesis of a gamma 3-melanotropin-like peptide in the pars intermedia of the amphibian pituitary gland.
1982,
Pubmed
,
Xenbase
Martens,
Expression of two proopiomelanocortin genes in the pituitary gland of Xenopus laevis: complete structures of the two preprohormones.
1986,
Pubmed
,
Xenbase
Marzioch,
Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.
1999,
Pubmed
Mironov,
ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains.
2003,
Pubmed
Muñiz,
The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles.
2000,
Pubmed
Nickel,
p23, a major COPI-vesicle membrane protein, constitutively cycles through the early secretory pathway.
1997,
Pubmed
Rojo,
Involvement of the transmembrane protein p23 in biosynthetic protein transport.
1997,
Pubmed
Rojo,
The transmembrane protein p23 contributes to the organization of the Golgi apparatus.
2000,
Pubmed
Roubos,
Background adaptation by Xenopus laevis: a model for studying neuronal information processing in the pituitary pars intermedia.
1997,
Pubmed
,
Xenbase
Rötter,
Cell-type-specific and selectively induced expression of members of the p24 family of putative cargo receptors.
2002,
Pubmed
,
Xenbase
Schimmöller,
The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi.
1995,
Pubmed
Schweizer,
Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus.
1988,
Pubmed
Sohn,
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding.
1996,
Pubmed
Sparrow,
A simplified method of generating transgenic Xenopus.
2000,
Pubmed
,
Xenbase
Springer,
The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae.
2000,
Pubmed
Stamnes,
An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.
1995,
Pubmed
Tuinhof,
Involvement of retinohypothalamic input, suprachiasmatic nucleus, magnocellular nucleus and locus coeruleus in control of melanotrope cells of Xenopus laevis: a retrograde and anterograde tracing study.
1994,
Pubmed
,
Xenbase
Van Horssen,
Manipulation of disulfide bonds differentially affects the intracellular transport, sorting, and processing of neuroendocrine secretory proteins.
1998,
Pubmed
,
Xenbase
Weatherhead,
Effects of change of background colour on the ultrastructure of the 'MSH cells' of the pars intermedia of Xenopus laevis.
1971,
Pubmed
,
Xenbase
Wen,
p24 proteins and quality control of LIN-12 and GLP-1 trafficking in Caenorhabditis elegans.
1999,
Pubmed
de Rijk,
Morphology of the pars intermedia and the melanophore-stimulating cells in Xenopus laevis in relation to background adaptation.
1990,
Pubmed
,
Xenbase
