Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Exp Biol
2010 May 01;213Pt 10:1782-7. doi: 10.1242/jeb.040568.
Show Gene links
Show Anatomy links
Stretch-activated cation channel from larval bullfrog skin.
Hillyard SD
,
Willumsen NJ
,
Marrero MB
.
???displayArticle.abstract???
Cell-attached patches from isolated epithelial cells from larval bullfrog skin revealed a cation channel that was activated by applying suction (-1 kPa to -4.5 kPa) to the pipette. Activation was characterized by an initial large current spike that rapidly attenuated to a stable value and showed a variable pattern of opening and closing with continuing suction. Current-voltage plots demonstrated linear or inward rectification and single channel conductances of 44-56 pS with NaCl or KCl Ringer's solution as the pipette solution, and a reversal potential (-V(p)) of 20-40 mV. The conductance was markedly reduced with N-methyl-D-glucamide (NMDG)-Cl Ringer's solution in the pipette. Neither amiloride nor ATP, which are known to stimulate an apical cation channel in Ussing chamber preparations of larval frog skin, produced channel activation nor did these compounds affect the response to suction. Stretch activation was not affected by varying the pipette concentrations of Ca(2+) between 0 mmol l(-1) and 4 mmol l(-1) or by varying pH between 6.8 and 8.0. However, conductance was reduced with 4 mmol l(-1) Ca(2+). Western blot analysis of membrane homogenates from larval bullfrog and larval toad skin identified proteins that were immunoreactive with mammalian TRPC1 and TRPC5 (TRPC, canonical transient receptor potential channel) antibodies while homogenates of skin from newly metamorphosed bullfrogs were positive for TRPC1 and TRPC3/6/7 antibodies. The electrophysiological response of larval bullfrog skin resembles that of a stretch-activated cation channel characterized in Xenopus oocytes and proposed to be TRPC1. These results indicate this channel persists in all life stages of anurans and that TRP isoforms may be important for sensory functions of their skin.
Alvarado,
Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana.
1970, Pubmed
Alvarado,
Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana.
1970,
Pubmed
Banes-Berceli,
Effect of simvastatin on high glucose- and angiotensin II-induced activation of the JAK/STAT pathway in mesangial cells.
2006,
Pubmed
Bobanovic,
Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus.
1999,
Pubmed
,
Xenbase
Bryan-Sisneros,
Electrophysiological, mechanosensitive responses of Xenopus laevis oocytes to direct, isotonic increase in intracellular volume.
2003,
Pubmed
,
Xenbase
Cox,
Electrical and transport characteristics of skin of larval Rana catesbeiana.
1979,
Pubmed
Cox,
Calcium channel blockers inhibit amiloride-stimulated short-circuit current in frog tadpole skin.
1992,
Pubmed
Cox,
Low-affinity mixed acetylcholine-responsive receptors at the apical membrane of frog tadpole skin.
1993,
Pubmed
Cox,
Apical regulation of nonselective cation channels by ATP in larval bullfrog skin.
1997,
Pubmed
Dietrich,
Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1.
2007,
Pubmed
Gomis,
Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels.
2008,
Pubmed
Gottlieb,
Revisiting TRPC1 and TRPC6 mechanosensitivity.
2008,
Pubmed
Hamill,
Rapid adaptation of single mechanosensitive channels in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Hillyard,
Basolateral Cl- channels in the larval bullfrog skin epithelium.
2002,
Pubmed
Hillyard,
A fluctuation analysis study of the development of amiloride-sensitive Na+ transport in the skin of larval bullfrogs (Rana catesbeiana).
1982,
Pubmed
Hillyard,
Effect of amiloride on the poorly selective cation channel of larval bullfrog skin.
1989,
Pubmed
Jensik,
Cloning and characterization of a functional P2X receptor from larval bullfrog skin.
2001,
Pubmed
,
Xenbase
Lane,
Amiloride block of the mechanosensitive cation channel in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Liu,
Molecular analysis of a store-operated and 2-acetyl-sn-glycerol-sensitive non-selective cation channel. Heteromeric assembly of TRPC1-TRPC3.
2005,
Pubmed
Lumpkin,
Mechanisms of sensory transduction in the skin.
2007,
Pubmed
Maroto,
TRPC1 forms the stretch-activated cation channel in vertebrate cells.
2005,
Pubmed
,
Xenbase
McBride,
Pressure-clamp: a method for rapid step perturbation of mechanosensitive channels.
1992,
Pubmed
,
Xenbase
Nilius,
Calcium-impermeable monovalent cation channels: a TRP connection?
2003,
Pubmed
Roberts,
Conducted impulses in the skin of young tadpoles.
1969,
Pubmed
Robinson,
Morphology of ventral epidermis of Rana catesbeiana during metamorphosis.
1987,
Pubmed
Strübing,
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
2001,
Pubmed
Taglietti,
A study of stretch-activated channels in the membrane of frog oocytes: interactions with Ca2+ ions.
1988,
Pubmed
Takada,
Corticoid-induced differentiation of amiloride-blockable active Na+ transport across larval bullfrog skin in vitro.
1995,
Pubmed
Takada,
Larval bullfrog skin expresses ENaC despite having no amiloride-blockable transepithelial Na+ transport.
2006,
Pubmed
Van Driessche,
Ca2+-sensitive, spontaneously fluctuating, cation channels in the apical membrane of the adult frog skin epithelium.
1985,
Pubmed
Wilkinson,
Effects of mechano-gated cation channel blockers on Xenopus oocyte growth and development.
1998,
Pubmed
,
Xenbase
