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Eur Biophys J
2004 Aug 01;335:412-20. doi: 10.1007/s00249-003-0375-y.
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Modeling of basolateral ATP release induced by hypotonic treatment in A6 cells.
Gheorghiu M
,
Van Driessche W
.
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ATP is released from the basolateral membrane of A6 epithelia in response to hypotonic treatment. This study addresses the problem of ATP diffusion through the permeable supports used to culture the cells. A theoretical analysis of a recently introduced experimental protocol is presented and a model of ATP diffusion through the compartments of the measuring system is proposed. The model provides the ATP profiles near the cell layer and in the measurement chamber. Comparison of results from computer simulations and experimental data showed that the permeable support introduces a marked delay for ATP diffusion, supporting the correlation of apparently time-separated events: the mobilization of Ca(2+) from internal stores and release of ATP from the cell. The model is consistent with experimental data obtained with the luciferin-luciferase pulse protocol and provides an indirect proof of related processes like the closure and opening of the lateral interspace that occur after imposing the hyposmotic shock. The influence of the pore structure of the permeable support in modulating the measured release rates revealed by computer simulation is experimentally validated for two types of Anopore filters.
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1997,
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Gomi,
Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin.
2001,
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Gordjani,
Capacitative Ca2+ entry (CCE) induced by luminal and basolateral ATP in polarised MDCK-C7 cells is restricted to the basolateral membrane.
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Grygorczyk,
CFTR-independent ATP release from epithelial cells triggered by mechanical stimuli.
1997,
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Hamada,
Stretch activates Jun N-terminal kinase/stress-activated protein kinase in vascular smooth muscle cells through mechanisms involving autocrine ATP stimulation of purinoceptors.
1998,
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Hazama,
Swelling-induced, CFTR-independent ATP release from a human epithelial cell line: lack of correlation with volume-sensitive cl(-) channels.
1999,
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Helman,
Substrate-dependent expression of Na+ transport and shunt conductance in A6 epithelia.
1997,
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,
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Hisadome,
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Homolya,
Cell to cell communication in response to mechanical stress via bilateral release of ATP and UTP in polarized epithelia.
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Jans,
Mg(2+)-sensitive non-capacitative basolateral Ca(2+) entry secondary to cell swelling in the polarized renal A6 epithelium.
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Jans,
Hypotonic treatment evokes biphasic ATP release across the basolateral membrane of cultured renal epithelia (A6).
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,
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Kimura,
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP.
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Knight,
ATP is released from guinea pig ureter epithelium on distension.
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Lange,
Regulation of cell volume via microvillar ion channels.
2000,
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Leipziger,
Control of epithelial transport via luminal P2 receptors.
2003,
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Mahoney,
Time course of the initial [Ca2+]i response to extracellular ATP in smooth muscle depends on [Ca2+]e and ATP concentration.
1998,
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Mitchell,
Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space.
2001,
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Ostrom,
Cellular release of and response to ATP as key determinants of the set-point of signal transduction pathways.
2000,
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Roman,
Hepatocellular ATP-binding cassette protein expression enhances ATP release and autocrine regulation of cell volume.
1997,
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Roman,
Evidence for multidrug resistance-1 P-glycoprotein-dependent regulation of cellular ATP permeability.
2001,
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Romanello,
Mechanically induced ATP release from human osteoblastic cells.
2001,
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Schwiebert,
Extracellular nucleotide signaling along the renal epithelium.
2001,
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Stout,
Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels.
2002,
Pubmed
Taylor,
Bioluminescence detection of ATP release mechanisms in epithelia.
1998,
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Van Driessche,
Transepithelial capacitance decrease reveals closure of lateral interspace in A6 epithelia.
1999,
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Van der Wijk,
Osmotic cell swelling-induced ATP release mediates the activation of extracellular signal-regulated protein kinase (Erk)-1/2 but not the activation of osmo-sensitive anion channels.
1999,
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Wang,
Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation.
1996,
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Woda,
Effects of luminal flow and nucleotides on [Ca(2+)](i) in rabbit cortical collecting duct.
2002,
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de Graaf,
In vivo (31)P-NMR diffusion spectroscopy of ATP and phosphocreatine in rat skeletal muscle.
2000,
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