Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Protoc
2010 May 01;55:841-8. doi: 10.1038/nprot.2010.10.
Show Gene links
Show Anatomy links
In vivo single-cell excitability probing of neuronal ensembles in the intact and awake developing Xenopus brain.
Dunfield D
,
Haas K
.
???displayArticle.abstract???
Sensory experience can elicit long-lasting plasticity of both single neurons and ensemble neural circuit response properties during embryonic development. To investigate their relationship, one must image functional responses of large neuronal populations simultaneously with single-cell resolution. In this protocol, we describe a noninvasive approach to assay functional plasticity of individual neurons and neuronal populations in vivo using targeted infusion of calcium-sensitive dyes, two-photon microscopy and synchronized visual stimuli presentations. This technique allows visualization of approximately 200 neurons while probing visual responses in the optic tectum of awake, immobilized Xenopus laevis tadpoles. The protocol includes visual training paradigms that elicit long-lasting potentiation or depression of functional responses, allowing investigations of population and single-neuron plasticity induced by natural sensory stimuli in the awake, intact, developing brain. Setup time for this protocol, including dye injection and chamber preparation, is approximately 2 h. Excitability probing experiments can then be performed for at least 3 h.
Aizenman,
Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo.
2003, Pubmed,
Xenbase
Aizenman,
Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo.
2003,
Pubmed
,
Xenbase
Aizenman,
Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines.
2002,
Pubmed
,
Xenbase
Bhatt,
Cyclic AMP-induced repair of zebrafish spinal circuits.
2004,
Pubmed
Brustein,
"In vivo" monitoring of neuronal network activity in zebrafish by two-photon Ca(2+) imaging.
2003,
Pubmed
Chen,
Heterosynaptic LTP in early development.
2001,
Pubmed
Cline,
The regulation of dendritic arbor development and plasticity by glutamatergic synaptic input: a review of the synaptotrophic hypothesis.
2008,
Pubmed
Dunfield,
Metaplasticity governs natural experience-driven plasticity of nascent embryonic brain circuits.
2009,
Pubmed
,
Xenbase
Engert,
Moving visual stimuli rapidly induce direction sensitivity of developing tectal neurons.
2002,
Pubmed
,
Xenbase
Espinosa,
Uncoupling dendrite growth and patterning: single-cell knockout analysis of NMDA receptor 2B.
2009,
Pubmed
Fetcho,
Monitoring activity in neuronal populations with single-cell resolution in a behaving vertebrate.
1998,
Pubmed
Gaze,
The evolution of the retinotectal map during development in Xenopus.
1974,
Pubmed
,
Xenbase
Gordon,
Experience-dependent plasticity of binocular responses in the primary visual cortex of the mouse.
1996,
Pubmed
Greenberg,
Population imaging of ongoing neuronal activity in the visual cortex of awake rats.
2008,
Pubmed
Haas,
AMPA receptors regulate experience-dependent dendritic arbor growth in vivo.
2006,
Pubmed
,
Xenbase
Johenning,
Nuclear calcium signals during L-LTP induction do not predict the degree of synaptic potentiation.
2007,
Pubmed
Junek,
Activity correlation imaging: visualizing function and structure of neuronal populations.
2009,
Pubmed
,
Xenbase
Kerr,
Imaging input and output of neocortical networks in vivo.
2005,
Pubmed
Mu,
Spike timing-dependent LTP/LTD mediates visual experience-dependent plasticity in a developing retinotectal system.
2006,
Pubmed
,
Xenbase
Niell,
Functional imaging reveals rapid development of visual response properties in the zebrafish tectum.
2005,
Pubmed
Ramdya,
Reverse correlation of rapid calcium signals in the zebrafish optic tectum in vivo.
2006,
Pubmed
Ramdya,
Emergence of binocular functional properties in a monocular neural circuit.
2008,
Pubmed
Ruthazer,
Control of axon branch dynamics by correlated activity in vivo.
2003,
Pubmed
,
Xenbase
SPERRY,
CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS.
1963,
Pubmed
Sakaguchi,
Map formation in the developing Xenopus retinotectal system: an examination of ganglion cell terminal arborizations.
1985,
Pubmed
,
Xenbase
Sasaki,
Fast and accurate detection of action potentials from somatic calcium fluctuations.
2008,
Pubmed
Sin,
Dendrite growth increased by visual activity requires NMDA receptor and Rho GTPases.
2002,
Pubmed
,
Xenbase
Smetters,
Detecting action potentials in neuronal populations with calcium imaging.
1999,
Pubmed
Sretavan,
Prenatal development of individual retinogeniculate axons during the period of segregation.
,
Pubmed
Stocca,
Differential dendritic Ca2+ signalling in young and mature hippocampal granule cells.
2008,
Pubmed
Stosiek,
In vivo two-photon calcium imaging of neuronal networks.
2003,
Pubmed
Sumbre,
Entrained rhythmic activities of neuronal ensembles as perceptual memory of time interval.
2008,
Pubmed
Takahashi,
Watching neuronal circuit dynamics through functional multineuron calcium imaging (fMCI).
2007,
Pubmed
Tao,
Emergence of input specificity of ltp during development of retinotectal connections in vivo.
2001,
Pubmed
,
Xenbase
Tao,
Activity-dependent matching of excitatory and inhibitory inputs during refinement of visual receptive fields.
2005,
Pubmed
,
Xenbase
Thévenaz,
A pyramid approach to subpixel registration based on intensity.
1998,
Pubmed
Vislay-Meltzer,
Spatiotemporal specificity of neuronal activity directs the modification of receptive fields in the developing retinotectal system.
2006,
Pubmed
,
Xenbase
WIESEL,
SINGLE-CELL RESPONSES IN STRIATE CORTEX OF KITTENS DEPRIVED OF VISION IN ONE EYE.
1963,
Pubmed
Wong,
Calcium imaging and multielectrode recordings of global patterns of activity in the developing nervous system.
1998,
Pubmed
Yaksi,
Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging.
2006,
Pubmed
Zhang,
Visual input induces long-term potentiation of developing retinotectal synapses.
2000,
Pubmed
,
Xenbase
Zhang,
A critical window for cooperation and competition among developing retinotectal synapses.
1998,
Pubmed
,
Xenbase
Zhou,
Reversal and stabilization of synaptic modifications in a developing visual system.
2003,
Pubmed
,
Xenbase
