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J Biol Chem
2010 May 28;28522:16739-47. doi: 10.1074/jbc.M109.053850.
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Ser123 is essential for the water channel activity of McPIP2;1 from Mesembryanthemum crystallinum.
Amezcua-Romero JC
,
Pantoja O
,
Vera-Estrella R
.
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The increased expression of McPIP2;1 (MipC), a root-specific aquaporin (AQP) from Mesembryanthemum crystallinum, under salt stress has suggested a role for this AQP in the salt tolerance of the plant. However, whether McPIP2;1 transports water or another solute and how its activity is regulated are so far unknown. Therefore, wild type (wt) or mutated McPIP2;1 protein was expressed in Xenopus laevis oocytes. Then, the osmotic water permeability (P(f)) of the oocytes membrane was assessed by hypotonic challenges. Selectivity of McPIP2;1 to water was determined by radiolabeled glycerol or urea uptake assays. Moreover, swelling and in vitro phosphorylation assays revealed that both water permeation and phosphorylation status of McPIP2;1 were significantly increased by the phosphorylation agonists okadaic acid (OA), phorbol myristate acetate (PMA), and 8-Br-cAMP, and markedly decreased by the inhibitory peptides PKI 14-22 and PKC 20-28, inhibitors of protein kinases A (PKA) and C (PKC), respectively. Substitution of Ser(123) or both, Ser(123) and Ser(282), abolished the water channel activity of McPIP2;1 while substitution of Ser(282) only partially inhibited it (51.9% inhibition). Despite lacking Ser(123) and/or Ser(282), the McPIP2;1 mutant forms were still phosphorylated in vitro, which suggests that phosphorylation may have a dual role on this AQP. Our results indicate that McPIP2;1 water permeability depends completely on Ser(123) and is positively regulated by PKA- and PKC-mediated phosphorylation. Regulation of the phosphorylation status of McPIP2;1 may contribute to control water transport through root cells when the plant is subjected to high salinity conditions.
Aroca,
The role of aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots.
2005, Pubmed
Aroca,
The role of aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots.
2005,
Pubmed
Beitz,
Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia, and protons.
2006,
Pubmed
,
Xenbase
Biela,
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
1999,
Pubmed
,
Xenbase
Calamita,
Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli.
1995,
Pubmed
,
Xenbase
Chaumont,
Regulation of plant aquaporin activity.
2005,
Pubmed
Daniels,
Phosphorylation of aquaporin PvTIP3;1 defined by mass spectrometry and molecular modeling.
2005,
Pubmed
Danielson,
Unexpected complexity of the aquaporin gene family in the moss Physcomitrella patens.
2008,
Pubmed
Eichholtz,
A myristoylated pseudosubstrate peptide, a novel protein kinase C inhibitor.
1993,
Pubmed
Fetter,
Interactions between plasma membrane aquaporins modulate their water channel activity.
2004,
Pubmed
,
Xenbase
Fu,
Structure of a glycerol-conducting channel and the basis for its selectivity.
2000,
Pubmed
Gerbeau,
Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes.
1999,
Pubmed
,
Xenbase
Gonen,
The structure of aquaporins.
2006,
Pubmed
,
Xenbase
Guenther,
Phosphorylation of soybean nodulin 26 on serine 262 enhances water permeability and is regulated developmentally and by osmotic signals.
2003,
Pubmed
,
Xenbase
Guex,
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
1997,
Pubmed
Gustavsson,
A novel plant major intrinsic protein in Physcomitrella patens most similar to bacterial glycerol channels.
2005,
Pubmed
,
Xenbase
Hachez,
Modulating the expression of aquaporin genes in planta: A key to understand their physiological functions?
2006,
Pubmed
Hanba,
Overexpression of the barley aquaporin HvPIP2;1 increases internal CO(2) conductance and CO(2) assimilation in the leaves of transgenic rice plants.
2004,
Pubmed
Harris,
Pseudosubstrate inhibition of cyclic AMP-dependent protein kinase in intact pancreatic islets: effects on cyclic AMP-dependent and glucose-dependent insulin secretion.
1997,
Pubmed
Haystead,
Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism.
1989,
Pubmed
Hei,
Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs.
1991,
Pubmed
Holm,
NH3 and NH4+ permeability in aquaporin-expressing Xenopus oocytes.
2005,
Pubmed
,
Xenbase
Horsefield,
High-resolution x-ray structure of human aquaporin 5.
2008,
Pubmed
Javot,
The role of aquaporins in root water uptake.
2002,
Pubmed
Johanson,
The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants.
2001,
Pubmed
Johansson,
Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.
1998,
Pubmed
,
Xenbase
Johansson,
The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential.
1996,
Pubmed
Johnson,
Tonoplast-bound protein kinase phosphorylates tonoplast intrinsic protein.
1992,
Pubmed
Kaldenhoff,
Functional aquaporin diversity in plants.
2006,
Pubmed
Kamiya,
NIP1;1, an aquaporin homolog, determines the arsenite sensitivity of Arabidopsis thaliana.
2009,
Pubmed
,
Xenbase
Katsuhara,
Barley plasma membrane intrinsic proteins (PIP Aquaporins) as water and CO2 transporters.
2008,
Pubmed
King,
From structure to disease: the evolving tale of aquaporin biology.
2004,
Pubmed
Kirch,
Expression of water channel proteins in Mesembryanthemum crystallinum.
2000,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Ma,
A silicon transporter in rice.
2006,
Pubmed
Maurel,
Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
1995,
Pubmed
,
Xenbase
Maurel,
Plant aquaporins: membrane channels with multiple integrated functions.
2008,
Pubmed
Melo,
Statistical potentials for fold assessment.
2002,
Pubmed
Miao,
Topology and phosphorylation of soybean nodulin-26, an intrinsic protein of the peribacteroid membrane.
1992,
Pubmed
Pieper,
MODBASE: a database of annotated comparative protein structure models and associated resources.
2006,
Pubmed
Prak,
Multiple phosphorylations in the C-terminal tail of plant plasma membrane aquaporins: role in subcellular trafficking of AtPIP2;1 in response to salt stress.
2008,
Pubmed
Sui,
Structural basis of water-specific transport through the AQP1 water channel.
,
Pubmed
Takano,
The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation.
2006,
Pubmed
,
Xenbase
Törnroth-Horsefield,
Structural mechanism of plant aquaporin gating.
2006,
Pubmed
Uehlein,
The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions.
2003,
Pubmed
,
Xenbase
Van Wilder,
Maize plasma membrane aquaporins belonging to the PIP1 and PIP2 subgroups are in vivo phosphorylated.
2008,
Pubmed
,
Xenbase
Vera-Estrella,
Novel regulation of aquaporins during osmotic stress.
2004,
Pubmed
,
Xenbase
Wallace,
Distinct transport selectivity of two structural subclasses of the nodulin-like intrinsic protein family of plant aquaglyceroporin channels.
2005,
Pubmed
,
Xenbase
Weaver,
Calcium-dependent phosphorylation of symbiosome membrane proteins from nitrogen-fixing soybean nodules : evidence for phosphorylation of nodulin-26.
1991,
Pubmed
Weaver,
Determination of the site of phosphorylation of nodulin 26 by the calcium-dependent protein kinase from soybean nodules.
1992,
Pubmed