Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2010 May 15;588Pt 10:1683-93. doi: 10.1113/jphysiol.2009.183418.
Show Gene links
Show Anatomy links
Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.
Martinez-Pinna J
,
Gurung IS
,
Mahaut-Smith MP
,
Morales A
.
???displayArticle.abstract???
Lysophosphatidic acid (LPA) G-protein-coupled receptors (GPCRs) play important roles in a variety of physiological and pathophysiological processes, including cell proliferation, angiogenesis, central nervous system development and carcinogenesis. Whilst many ion channels and transporters are recognized to be controlled by a change in cell membrane potential, little is known about the voltage dependence of other proteins involved in cell signalling. Here, we show that the InsP(3)-mediated Ca(2+) response stimulated by the endogenous LPA GPCR in Xenopus oocytes is potentiated by membrane depolarization. Depolarization was able to repetitively stimulate transient [Ca(2+)](i) increases after the initial agonist-evoked response. In addition, the initial rate and amplitude of the LPA-dependent Ca(2+) response were significantly modulated by the steady holding potential over the physiological range, such that the response to LPA was potentiated at depolarized potentials and inhibited at hyperpolarized potentials. Enhancement of LPA receptor-evoked Ca(2+) mobilization by membrane depolarization was observed over a wide range of agonist concentrations. Importantly, the amplitude of the depolarization-evoked intracellular Ca(2+) increase displayed an inverse relationship with agonist concentration such that the greatest effect of voltage was observed at near-threshold levels of agonist. Voltage-dependent Ca(2+) release was not induced by direct elevation of InsP(3) or by activation of heterotrimeric G-proteins in the absence of agonist, indicating that the LPA GPCR itself represents the primary site of action of membrane voltage. This novel modulation of LPA signalling by membrane potential may have important consequences for control of Ca(2+) signals both in excitable and non-excitable tissues.
An,
Recombinant human G protein-coupled lysophosphatidic acid receptors mediate intracellular calcium mobilization.
1998, Pubmed,
Xenbase
An,
Recombinant human G protein-coupled lysophosphatidic acid receptors mediate intracellular calcium mobilization.
1998,
Pubmed
,
Xenbase
Baker,
Direct quantitative analysis of lysophosphatidic acid molecular species by stable isotope dilution electrospray ionization liquid chromatography-mass spectrometry.
2001,
Pubmed
Ben-Chaim,
The M2 muscarinic G-protein-coupled receptor is voltage-sensitive.
2003,
Pubmed
,
Xenbase
Ben-Chaim,
Movement of 'gating charge' is coupled to ligand binding in a G-protein-coupled receptor.
2006,
Pubmed
,
Xenbase
Berridge,
Inositol trisphosphate and calcium signalling.
1993,
Pubmed
Bezanilla,
The voltage sensor in voltage-dependent ion channels.
2000,
Pubmed
Billups,
Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.
2006,
Pubmed
Chorna-Ornan,
A common mechanism underlies vertebrate calcium signaling and Drosophila phototransduction.
2001,
Pubmed
,
Xenbase
Chun,
International Union of Pharmacology. XXXIV. Lysophospholipid receptor nomenclature.
2002,
Pubmed
Clapham,
Calcium signaling.
1995,
Pubmed
Drummond,
Reporting ethical matters in the Journal of Physiology: standards and advice.
2009,
Pubmed
Fernhout,
Lysophosphatidic acid induces inward currents in Xenopus laevis oocytes; evidence for an extracellular site of action.
1992,
Pubmed
,
Xenbase
Fiske,
Voltage-sensitive ion channels and cancer.
2006,
Pubmed
Fukushima,
Lysophospholipid receptors.
2001,
Pubmed
Ganitkevich VYa,
Membrane potential modulates inositol 1,4,5-trisphosphate-mediated Ca2+ transients in guinea-pig coronary myocytes.
1993,
Pubmed
Gurung,
Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors.
2008,
Pubmed
Ishii,
Lysophospholipid receptors: signaling and biology.
2004,
Pubmed
Ivorra,
Membrane currents in immature oocytes of the Rana perezi frog.
1997,
Pubmed
,
Xenbase
Kimura,
Two novel Xenopus homologs of mammalian LP(A1)/EDG-2 function as lysophosphatidic acid receptors in Xenopus oocytes and mammalian cells.
2001,
Pubmed
,
Xenbase
Kingsbury,
Non-proliferative effects of lysophosphatidic acid enhance cortical growth and folding.
2003,
Pubmed
Kiselyov,
Functional interaction between InsP3 receptors and store-operated Htrp3 channels.
1998,
Pubmed
Kusano,
Acetylcholine receptors in the oocyte membrane.
,
Pubmed
,
Xenbase
Liliom,
Xenopus oocytes express multiple receptors for LPA-like lipid mediators.
1996,
Pubmed
,
Xenbase
Liu,
Membrane depolarization causes a direct activation of G protein-coupled receptors leading to local Ca2+ release in smooth muscle.
2009,
Pubmed
Lloyd,
Lysophosphatidic acid signaling controls cortical actin assembly and cytoarchitecture in Xenopus embryos.
2005,
Pubmed
,
Xenbase
Mahaut-Smith,
Properties of the demarcation membrane system in living rat megakaryocytes.
2003,
Pubmed
Mahaut-Smith,
Depolarization-evoked Ca2+ release in a non-excitable cell, the rat megakaryocyte.
1999,
Pubmed
Mahaut-Smith,
A role for membrane potential in regulating GPCRs?
2008,
Pubmed
Martinez-Pinna,
Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte.
2004,
Pubmed
Martinez-Pinna,
Direct voltage control of signaling via P2Y1 and other Galphaq-coupled receptors.
2005,
Pubmed
Marty,
The initiation of calcium release following muscarinic stimulation in rat lacrimal glands.
1989,
Pubmed
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Miledi,
Latencies of membrane currents evoked in Xenopus oocytes by receptor activation, inositol trisphosphate and calcium.
1989,
Pubmed
,
Xenbase
Mills,
The emerging role of lysophosphatidic acid in cancer.
2003,
Pubmed
Moolenaar,
The ins and outs of lysophosphatidic acid signaling.
2004,
Pubmed
Moon,
G-protein activation, intracellular Ca2+ mobilization and phosphorylation studies of membrane currents induced by AlF4- in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Noguchi,
Lysophosphatidic acid (LPA) and its receptors.
2009,
Pubmed
Noh,
Different signaling pathway between sphingosine-1-phosphate and lysophosphatidic acid in Xenopus oocytes: functional coupling of the sphingosine-1-phosphate receptor to PLC-xbeta in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Ohana,
The metabotropic glutamate G-protein-coupled receptors mGluR3 and mGluR1a are voltage-sensitive.
2006,
Pubmed
,
Xenbase
Parekh,
Store-operated calcium channels.
2005,
Pubmed
Parker,
Nonlinearity and facilitation in phosphoinositide signaling studied by the use of caged inositol trisphosphate in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Pierce,
Seven-transmembrane receptors.
2002,
Pubmed
Putney,
A model for receptor-regulated calcium entry.
1986,
Pubmed
Sahlholm,
Voltage-dependence of the human dopamine D2 receptor.
2008,
Pubmed
,
Xenbase
Schlief,
H2O2-induced chloride currents are indicative of an endogenous Na(+)-Ca2+ exchange mechanism in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Sternweis,
Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
1982,
Pubmed
Tigyi,
Lysophosphatidates bound to serum albumin activate membrane currents in Xenopus oocytes and neurite retraction in PC12 pheochromocytoma cells.
1992,
Pubmed
,
Xenbase
Zhang,
On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.
2000,
Pubmed
,
Xenbase