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XB-ART-41083
Methods 2010 May 01;511:101-5. doi: 10.1016/j.ymeth.2010.01.031.
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Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytes.

Pratt CA , Mowry KL .


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Understanding mechanisms of post-transcriptional control of gene expression has come under much scrutiny in recent years. A key question in this field is how the translation of specific mRNAs is activated or repressed both spatially and temporally in a given cell. In oocytes of the frog Xenopus laevis a number of mRNAs are localized early in oogenesis and subsequently translated at later stages. We have developed a highly active cell-free translation system from oocytes in the early stages of oogenesis that is applicable to the study of translation and translational control of both endogenous and exogenous mRNAs.

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References [+] :
Acker, Reconstitution of yeast translation initiation. 2007, Pubmed