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XB-ART-40799
Methods 2010 May 01;511:27-36. doi: 10.1016/j.ymeth.2009.12.009.
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Raman spectroscopy of Xenopus laevis oocytes.

Rusciano G , Pesce G , Salemme M , Selvaggi L , Vaccaro C , Sasso A .


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This work reports on the application of Raman spectroscopy for the analysis of Xenopus laevis oocytes (stage-I). A two-color home-made microscope has been used for this investigation. In particular, a 785nm Raman probe has been used to acquire the spontaneous Raman scattering from the oocyte cytoplasm, while a 532nm probe has been employed to detect carotenoids through Resonant Raman Scattering. Finally, the distribution of beta-carotene along a diameter of a single oocyte has been investigated.

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