Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Signal Behav
2007 Jul 01;24:255-7. doi: 10.4161/psb.2.4.3868.
Show Gene links
Show Anatomy links
The BnALMT1 Protein That is an Aluminum-Activated Malate Transporter is Localized in the Plasma Membrane.
Ligaba A
,
Katsuhara M
,
Sakamoto W
,
Matsumoto H
.
???displayArticle.abstract???
We have previously reported that Al-induces citrate and malate efflux from P-sufficient and P-deficient plants of rape (Brassica napus L.) and that P-deficiency alone could not induce this response. Further investigation showed that the transcript of two genes designated BnALMT1 and BnALMT2 is accumulated in roots by Al-treatment. Transgenic tobacco cells (Nicotiana tabacum) and Xenopus laevis oocytes expressing the BnALMT1 and BnALMT2 proteins released more malate than control cells in the presence of Al, indicating that the BnALMT genes encode an Al-activated malate transporter. The transgenic tobacco cells exposed to toxic level of Al grew better than control cells indicating that the genes can enhance Al-resistance of plant cells. In this study we showed the subcellular localization of BnALMT1 fused to the green fluoresce protein (GFP). The BnALMT1:: GFP construct was transiently expressed in protoplasts prepared from Arabidopsis leaves using the polyethylene glycol (PEG) method. The result showed that the BnALMT1 protein is localized in the plasma membrane. This provides further evidence that the BnALMT proteins facilitate the transport of malate across the plasma membrane (PM).
Delhaize,
Aluminum Tolerance in Wheat (Triticum aestivum L.) (II. Aluminum-Stimulated Excretion of Malic Acid from Root Apices).
1993, Pubmed
Delhaize,
Aluminum Tolerance in Wheat (Triticum aestivum L.) (II. Aluminum-Stimulated Excretion of Malic Acid from Root Apices).
1993,
Pubmed
Delhaize,
Engineering high-level aluminum tolerance in barley with the ALMT1 gene.
2004,
Pubmed
Henn,
Mosaicism in Turner's syndrome.
1998,
Pubmed
Hoekenga,
AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis.
2006,
Pubmed
,
Xenbase
Jian Zheng S,
High aluminum resistance in buckwheat. I. Al-induced specific secretion of oxalic acid from root tips.
1998,
Pubmed
Ligaba,
The role of phosphorus in aluminium-induced citrate and malate exudation from rape (Brassica napus).
2004,
Pubmed
Ligaba,
The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells.
2006,
Pubmed
,
Xenbase
Lipton,
Citrate, Malate, and Succinate Concentration in Exudates from P-Sufficient and P-Stressed Medicago sativa L. Seedlings.
1987,
Pubmed
Magalhaes,
Comparative mapping of a major aluminum tolerance gene in sorghum and other species in the poaceae.
2004,
Pubmed
Miyasaka,
Mechanism of aluminum tolerance in snapbeans : root exudation of citric Acid.
1991,
Pubmed
Raman,
Molecular characterization and mapping of ALMT1, the aluminium-tolerance gene of bread wheat (Triticum aestivum L.).
2005,
Pubmed
Ryan,
Aluminum activates an anion channel in the apical cells of wheat roots.
1997,
Pubmed
Sakamoto,
Coordinated regulation and complex formation of yellow variegated1 and yellow variegated2, chloroplastic FtsH metalloproteases involved in the repair cycle of photosystem II in Arabidopsis thylakoid membranes.
2003,
Pubmed
Sasaki,
A wheat gene encoding an aluminum-activated malate transporter.
2004,
Pubmed
,
Xenbase
Yamaguchi,
Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1).
2005,
Pubmed
Zhang,
Malate-permeable channels and cation channels activated by aluminum in the apical cells of wheat roots.
2001,
Pubmed
